Extensive monitoring of the natural menstrual cycle using the serum biomarkers estradiol, luteinizing hormone and progesterone
Expected values for estradiol (E2), luteinizing hormone (LH), and progesterone determined in serum allow accurate assessment of menstrual cycle phase. Automated immunoassays demonstrate variable degrees of bias, emphasizing the need to establish method-specific reference values. We therefore establi...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-05-01
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| Series: | Practical Laboratory Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551721000111 |
| _version_ | 1819135678175248384 |
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| author | Ellen Anckaert Alexander Jank Julia Petzold Felix Rohsmann Rhonda Paris Martin Renggli Kathrin Schönfeld Johan Schiettecatte Monika Kriner |
| author_facet | Ellen Anckaert Alexander Jank Julia Petzold Felix Rohsmann Rhonda Paris Martin Renggli Kathrin Schönfeld Johan Schiettecatte Monika Kriner |
| author_sort | Ellen Anckaert |
| collection | DOAJ |
| description | Expected values for estradiol (E2), luteinizing hormone (LH), and progesterone determined in serum allow accurate assessment of menstrual cycle phase. Automated immunoassays demonstrate variable degrees of bias, emphasizing the need to establish method-specific reference values. We therefore established method-specific reference intervals for the Elecsys® LH assay and new generation Elecsys Estradiol III and Progesterone III assays (cobas e 801 analyzer) in 85 apparently healthy women aged 22–37 (US)/18–37 (EU) years over one natural menstrual cycle. Cycle length and day of ovulation were standardized; phases were defined by LH surge and/or progesterone/E2 levels. Median (5th–95th percentile) concentrations (follicular/ovulation/luteal) were E2: 198 pmol/L (114–332), 757 pmol/L (222–1959) and 412 pmol/L (222–854); LH: 7.14 IU/L (4.78–13.2), 22.6 IU/L (8.11–72.7) and 6.24 IU/L (2.73–13.1); progesterone: 0.212 nmol/L (0.159–0.616), 1.81 nmol/L (0.175–13.2) and 28.8 nmol/L (13.1–46.3). Sub-phase (early/intermediate/late) reference values were also determined for follicular and luteal phases. This multicenter study established reliable, method-specific E2, LH and progesterone reference values that could assist clinical decision-making in women with fertility disorders and monitoring of natural cycles in assisted reproductive treatment. |
| first_indexed | 2024-12-22T10:22:54Z |
| format | Article |
| id | doaj.art-c0a16501e2e44cafb4b16aedec924208 |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-12-22T10:22:54Z |
| publishDate | 2021-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-c0a16501e2e44cafb4b16aedec9242082022-12-21T18:29:35ZengElsevierPractical Laboratory Medicine2352-55172021-05-0125e00211Extensive monitoring of the natural menstrual cycle using the serum biomarkers estradiol, luteinizing hormone and progesteroneEllen Anckaert0Alexander Jank1Julia Petzold2Felix Rohsmann3Rhonda Paris4Martin Renggli5Kathrin Schönfeld6Johan Schiettecatte7Monika Kriner8Laboratory of Hormonology and Tumour Markers, University Hospital Brussels (UZ Brussel), Free University of Brussels, Laarbeeklaan 101, B-1090, Brussels, Belgium; Corresponding author.University Hospital Leipzig, Liebigstraße 20, 04103, Leipzig, GermanyUniversity Hospital Leipzig, Liebigstraße 20, 04103, Leipzig, GermanyPraxis Dr Felix Rohsmann, Sindelsdorfer Str. 12, 82392, Habach, GermanyRoche Diagnostics, 9115 Hague Rd, 46256, Indianapolis, IN, USATrina Bioreactives AG, Grabenstrasse 8, 8606, Nänikon, SwitzerlandRoche Diagnostics GmbH, Nonnenwald 2, 82377, Penzberg, GermanyLaboratory of Hormonology and Tumour Markers, University Hospital Brussels (UZ Brussel), Free University of Brussels, Laarbeeklaan 101, B-1090, Brussels, BelgiumTriga-S, Mühltal 5, 82392, Habach, GermanyExpected values for estradiol (E2), luteinizing hormone (LH), and progesterone determined in serum allow accurate assessment of menstrual cycle phase. Automated immunoassays demonstrate variable degrees of bias, emphasizing the need to establish method-specific reference values. We therefore established method-specific reference intervals for the Elecsys® LH assay and new generation Elecsys Estradiol III and Progesterone III assays (cobas e 801 analyzer) in 85 apparently healthy women aged 22–37 (US)/18–37 (EU) years over one natural menstrual cycle. Cycle length and day of ovulation were standardized; phases were defined by LH surge and/or progesterone/E2 levels. Median (5th–95th percentile) concentrations (follicular/ovulation/luteal) were E2: 198 pmol/L (114–332), 757 pmol/L (222–1959) and 412 pmol/L (222–854); LH: 7.14 IU/L (4.78–13.2), 22.6 IU/L (8.11–72.7) and 6.24 IU/L (2.73–13.1); progesterone: 0.212 nmol/L (0.159–0.616), 1.81 nmol/L (0.175–13.2) and 28.8 nmol/L (13.1–46.3). Sub-phase (early/intermediate/late) reference values were also determined for follicular and luteal phases. This multicenter study established reliable, method-specific E2, LH and progesterone reference values that could assist clinical decision-making in women with fertility disorders and monitoring of natural cycles in assisted reproductive treatment.http://www.sciencedirect.com/science/article/pii/S2352551721000111Luteinizing hormoneEstradiolProgesteroneNormo-ovulatoryNatural menstrual cycle |
| spellingShingle | Ellen Anckaert Alexander Jank Julia Petzold Felix Rohsmann Rhonda Paris Martin Renggli Kathrin Schönfeld Johan Schiettecatte Monika Kriner Extensive monitoring of the natural menstrual cycle using the serum biomarkers estradiol, luteinizing hormone and progesterone Practical Laboratory Medicine Luteinizing hormone Estradiol Progesterone Normo-ovulatory Natural menstrual cycle |
| title | Extensive monitoring of the natural menstrual cycle using the serum biomarkers estradiol, luteinizing hormone and progesterone |
| title_full | Extensive monitoring of the natural menstrual cycle using the serum biomarkers estradiol, luteinizing hormone and progesterone |
| title_fullStr | Extensive monitoring of the natural menstrual cycle using the serum biomarkers estradiol, luteinizing hormone and progesterone |
| title_full_unstemmed | Extensive monitoring of the natural menstrual cycle using the serum biomarkers estradiol, luteinizing hormone and progesterone |
| title_short | Extensive monitoring of the natural menstrual cycle using the serum biomarkers estradiol, luteinizing hormone and progesterone |
| title_sort | extensive monitoring of the natural menstrual cycle using the serum biomarkers estradiol luteinizing hormone and progesterone |
| topic | Luteinizing hormone Estradiol Progesterone Normo-ovulatory Natural menstrual cycle |
| url | http://www.sciencedirect.com/science/article/pii/S2352551721000111 |
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