Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep
Contagious ecthyma (Orf), an acute and highly contagious zoonosis, is prevalent worldwide. Orf is caused by Orf virus (ORFV), which mainly infects sheep/goats and humans. Therefore, effective and safe vaccination strategies for Orf prevention are needed. Although immunization with single-type Orf va...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1077938/full |
| _version_ | 1797866935145201664 |
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| author | Yan Wang Shihui Sun Kui Zhao Le Du Xinyue Wang Wenqi He Feng Gao Deguang Song Jiyu Guan |
| author_facet | Yan Wang Shihui Sun Kui Zhao Le Du Xinyue Wang Wenqi He Feng Gao Deguang Song Jiyu Guan |
| author_sort | Yan Wang |
| collection | DOAJ |
| description | Contagious ecthyma (Orf), an acute and highly contagious zoonosis, is prevalent worldwide. Orf is caused by Orf virus (ORFV), which mainly infects sheep/goats and humans. Therefore, effective and safe vaccination strategies for Orf prevention are needed. Although immunization with single-type Orf vaccines has been tested, heterologous prime-boost strategies still need to be studied. In the present study, ORFV B2L and F1L were selected as immunogens, based on which DNA, subunit and adenovirus vaccine candidates were generated. Of note, heterologous immunization strategies using DNA prime-protein boost and DNA prime-adenovirus boost in mice were performed, with single-type vaccines as controls. We have found that the DNA prime-protein boost strategy induces stronger humoral and cellular immune responses than DNA prime-adenovirus boost strategy in mice, which was confirmed by the changes in specific antibodies, lymphocyte proliferation and cytokine expression. Importantly, this observation was also confirmed when these heterologous immunization strategies were performed in sheep. In summary, by comparing the two immune strategies, we found that DNA prime-protein boost strategy can induce a better immune response, which provides a new attempt for exploring Orf immunization strategy. |
| first_indexed | 2024-04-09T23:32:12Z |
| format | Article |
| id | doaj.art-c0a399322b5e4a058458eeee21451492 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-09T23:32:12Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-c0a399322b5e4a058458eeee214514922023-03-21T04:25:25ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-03-011410.3389/fimmu.2023.10779381077938Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheepYan WangShihui SunKui ZhaoLe DuXinyue WangWenqi HeFeng GaoDeguang SongJiyu GuanContagious ecthyma (Orf), an acute and highly contagious zoonosis, is prevalent worldwide. Orf is caused by Orf virus (ORFV), which mainly infects sheep/goats and humans. Therefore, effective and safe vaccination strategies for Orf prevention are needed. Although immunization with single-type Orf vaccines has been tested, heterologous prime-boost strategies still need to be studied. In the present study, ORFV B2L and F1L were selected as immunogens, based on which DNA, subunit and adenovirus vaccine candidates were generated. Of note, heterologous immunization strategies using DNA prime-protein boost and DNA prime-adenovirus boost in mice were performed, with single-type vaccines as controls. We have found that the DNA prime-protein boost strategy induces stronger humoral and cellular immune responses than DNA prime-adenovirus boost strategy in mice, which was confirmed by the changes in specific antibodies, lymphocyte proliferation and cytokine expression. Importantly, this observation was also confirmed when these heterologous immunization strategies were performed in sheep. In summary, by comparing the two immune strategies, we found that DNA prime-protein boost strategy can induce a better immune response, which provides a new attempt for exploring Orf immunization strategy.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1077938/fullOrf virusDNA prime-protein boost vaccinationDNA prime-adenovirus boost vaccinationimmune responsesOrf immunization strategy |
| spellingShingle | Yan Wang Shihui Sun Kui Zhao Le Du Xinyue Wang Wenqi He Feng Gao Deguang Song Jiyu Guan Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep Frontiers in Immunology Orf virus DNA prime-protein boost vaccination DNA prime-adenovirus boost vaccination immune responses Orf immunization strategy |
| title | Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
| title_full | Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
| title_fullStr | Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
| title_full_unstemmed | Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
| title_short | Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
| title_sort | orf virus dna prime protein boost strategy is superior to adenovirus based vaccination in mice and sheep |
| topic | Orf virus DNA prime-protein boost vaccination DNA prime-adenovirus boost vaccination immune responses Orf immunization strategy |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1077938/full |
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