The Application of PMA (Propidium Monoazide) to Different Target Sequence Lengths of Zebrafish eDNA: A New Approach Aimed Toward Improving Environmental DNA Ecology and Biological Surveillance

The Application of PMA (Propidium Monoazide) to Different Target Sequence Lengths of Zebrafish eDNA: A New Approach Aimed Toward Improving Environmental DNA Ecology and Biological Surveillance

Environmental DNA (eDNA) analysis has enabled more sensitive and efficient biological monitoring than traditional methods. However, since the target species is not directly observed, interpretation of results cannot preclude process Type I errors. Specifically, there may be a spatial or temporal gap...

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Bibliographic Details
Main Authors: Takaya Hirohara, Kenji Tsuri, Koichi Miyagawa, Robert T. R. Paine, Hiroki Yamanaka
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-06-01
Series:Frontiers in Ecology and Evolution
Subjects:
environmental DNA
decay rate
propidium monoazide
quantitative PCR
zebrafish
Online Access:https://www.frontiersin.org/articles/10.3389/fevo.2021.632973/full

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