An investigation of the assumed efficacy of methionine hydroxy analogue compared to DL-methionine by measuring growth performance, carcass traits, and GHR and IGF-I expression in broilers
This experiment was conducted to investigate the assumed efficacy of 65% on the product basis of methionine hydroxy analog (OH-Met, 88% aqueous solution of DL-2-hydroxy-4-(methylthio) butanoic acid) relative to DL-methionine (DL-Met) in broiler chickens. A total of 792-day-old male chickens were ran...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/1828051X.2023.2271056 |
| _version_ | 1797198457939689472 |
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| author | Engin Yenice Ali Anıl Çenesiz İsmail Yavaş Neşe Nuray Toprak İbrahim Çiftci Necmettin Ceylan |
| author_facet | Engin Yenice Ali Anıl Çenesiz İsmail Yavaş Neşe Nuray Toprak İbrahim Çiftci Necmettin Ceylan |
| author_sort | Engin Yenice |
| collection | DOAJ |
| description | This experiment was conducted to investigate the assumed efficacy of 65% on the product basis of methionine hydroxy analog (OH-Met, 88% aqueous solution of DL-2-hydroxy-4-(methylthio) butanoic acid) relative to DL-methionine (DL-Met) in broiler chickens. A total of 792-day-old male chickens were randomly allotted to 7 dietary treatments consisting of a methionine deficient-basal diet based on corn-soybean meal and 6 different diets obtained by supplying the basal diet with methionine at 3 levels (25, 100 and 125%; levels of addition relative to the required amount of additional Met to meet SID Met + Cys requirements) from either DL-Met or OH-Met at a ratio of 65:100 (DL-Met to OH-Met on a product basis) in the corresponding treatments. Regardless of the source, methionine supplementation enhanced (p < .05) growth performance, carcase and breast meat yield. Supplemental level and the source of Met had no significant effect on the proximate chemical composition of breast meat (p > .05). Comparison of effects of OH-Met and DL-Met supplemented at 100–65% weight ratio revealed no differences at any Met + Cys level and for performance criterion confirming the applied concept. Expression of growth hormone receptor (GHR) mRNA, which showed a positive correlation with body weight gain, carcase yield and breast percentage, in broiler liver significantly increased with 100% and 125% relative addition of DL-Met, while only 100% of OH-Met addition did (p < .05) when compared to the basal diet. The expression of the insulin-like growth factor-I (IGF-I) gene was not significantly affected by either Met source or supplementation level. In conclusion, our data indicated that DL-Met can be substituted with a 1.54 times higher amount of OH-Met in corn-soybean meal based broiler diets. |
| first_indexed | 2024-03-08T16:59:13Z |
| format | Article |
| id | doaj.art-c0a60b742e034e42b70357b6a48d33df |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-04-24T07:00:10Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-c0a60b742e034e42b70357b6a48d33df2024-04-22T08:52:29ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2023-12-012211083109410.1080/1828051X.2023.22710562271056An investigation of the assumed efficacy of methionine hydroxy analogue compared to DL-methionine by measuring growth performance, carcass traits, and GHR and IGF-I expression in broilersEngin Yenice0Ali Anıl Çenesiz1İsmail Yavaş2Neşe Nuray Toprak3İbrahim Çiftci4Necmettin Ceylan5Department of Animal Science, Faculty of Agriculture, Ankara UniversityDepartment of Animal Science, Faculty of Agriculture, Ankara UniversityDepartment of Animal Science, Faculty of Agriculture, Ankara UniversityDepartment of Animal Science, Faculty of Agriculture, Ankara UniversityDepartment of Animal Science, Faculty of Agriculture, Ankara UniversityDepartment of Animal Science, Faculty of Agriculture, Ankara UniversityThis experiment was conducted to investigate the assumed efficacy of 65% on the product basis of methionine hydroxy analog (OH-Met, 88% aqueous solution of DL-2-hydroxy-4-(methylthio) butanoic acid) relative to DL-methionine (DL-Met) in broiler chickens. A total of 792-day-old male chickens were randomly allotted to 7 dietary treatments consisting of a methionine deficient-basal diet based on corn-soybean meal and 6 different diets obtained by supplying the basal diet with methionine at 3 levels (25, 100 and 125%; levels of addition relative to the required amount of additional Met to meet SID Met + Cys requirements) from either DL-Met or OH-Met at a ratio of 65:100 (DL-Met to OH-Met on a product basis) in the corresponding treatments. Regardless of the source, methionine supplementation enhanced (p < .05) growth performance, carcase and breast meat yield. Supplemental level and the source of Met had no significant effect on the proximate chemical composition of breast meat (p > .05). Comparison of effects of OH-Met and DL-Met supplemented at 100–65% weight ratio revealed no differences at any Met + Cys level and for performance criterion confirming the applied concept. Expression of growth hormone receptor (GHR) mRNA, which showed a positive correlation with body weight gain, carcase yield and breast percentage, in broiler liver significantly increased with 100% and 125% relative addition of DL-Met, while only 100% of OH-Met addition did (p < .05) when compared to the basal diet. The expression of the insulin-like growth factor-I (IGF-I) gene was not significantly affected by either Met source or supplementation level. In conclusion, our data indicated that DL-Met can be substituted with a 1.54 times higher amount of OH-Met in corn-soybean meal based broiler diets.http://dx.doi.org/10.1080/1828051X.2023.2271056broilergene expressionefficacy of methionine hydroxy analog |
| spellingShingle | Engin Yenice Ali Anıl Çenesiz İsmail Yavaş Neşe Nuray Toprak İbrahim Çiftci Necmettin Ceylan An investigation of the assumed efficacy of methionine hydroxy analogue compared to DL-methionine by measuring growth performance, carcass traits, and GHR and IGF-I expression in broilers Italian Journal of Animal Science broiler gene expression efficacy of methionine hydroxy analog |
| title | An investigation of the assumed efficacy of methionine hydroxy analogue compared to DL-methionine by measuring growth performance, carcass traits, and GHR and IGF-I expression in broilers |
| title_full | An investigation of the assumed efficacy of methionine hydroxy analogue compared to DL-methionine by measuring growth performance, carcass traits, and GHR and IGF-I expression in broilers |
| title_fullStr | An investigation of the assumed efficacy of methionine hydroxy analogue compared to DL-methionine by measuring growth performance, carcass traits, and GHR and IGF-I expression in broilers |
| title_full_unstemmed | An investigation of the assumed efficacy of methionine hydroxy analogue compared to DL-methionine by measuring growth performance, carcass traits, and GHR and IGF-I expression in broilers |
| title_short | An investigation of the assumed efficacy of methionine hydroxy analogue compared to DL-methionine by measuring growth performance, carcass traits, and GHR and IGF-I expression in broilers |
| title_sort | investigation of the assumed efficacy of methionine hydroxy analogue compared to dl methionine by measuring growth performance carcass traits and ghr and igf i expression in broilers |
| topic | broiler gene expression efficacy of methionine hydroxy analog |
| url | http://dx.doi.org/10.1080/1828051X.2023.2271056 |
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