Characterization of N-Linked Glycosylation in a Monoclonal Antibody Produced in NS0 Cells Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Characterization of N-Linked Glycosylation in a Monoclonal Antibody Produced in NS0 Cells Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection

The N-linked glycosylation in recombinant monoclonal antibodies (mAb) occurs at Asn297 on the Fc region in the CH2 domain. Glycosylation heterogeneities have been well documented to affect biological activities such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxi...

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Bibliographic Details
Main Authors: Yang Wang, Melissa Hamm, Richard R. Rustandi
Format: Article
Language:English
Published: MDPI AG 2013-03-01
Series:Pharmaceuticals
Subjects:
CE-LIF
APTS
HILIC-HPLC
glycans
monoclonal antibody NS0
capillary electrophoresis
Online Access:http://www.mdpi.com/1424-8247/6/3/393

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