The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway
Abstract Soybean protein‐derived peptides (SBP) are a rich source of various bioactive peptides with multiple health benefits. However, the prospective effects of SBP on human cells are still unclear. Therefore, this article investigated the effects of small molecular weight SBP on MG132‐induced apo...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-08-01
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| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.1776 |
| _version_ | 1797661210353598464 |
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| author | Guofu Yi He Li You Li Fen Zhao Zhiwei Ying Menglan Liu Jian Zhang Xinqi Liu |
| author_facet | Guofu Yi He Li You Li Fen Zhao Zhiwei Ying Menglan Liu Jian Zhang Xinqi Liu |
| author_sort | Guofu Yi |
| collection | DOAJ |
| description | Abstract Soybean protein‐derived peptides (SBP) are a rich source of various bioactive peptides with multiple health benefits. However, the prospective effects of SBP on human cells are still unclear. Therefore, this article investigated the effects of small molecular weight SBP on MG132‐induced apoptosis in RAW264.7 cells. SBP inhibited MG132‐induced apoptosis of RAW264.7 cells in a dose‐dependent manner by flow cytometry. To further study its molecular mechanisms, Western blot analysis demonstrated that SBP could activate the PI3K‐AKT pathway by increasing the phosphorylation of PI3K and AKT and inhibiting apoptosis pathway by downregulating the expressions of pro‐apoptotic proteins of Bim, Bax, Fas, and Fasl and promoting the expressions of anti‐apoptotic proteins of Bcl‐xL and Bcl‐2. These results indicated the protective effect of SBP on MG132‐induced apoptosis in RAW264.7 cells. |
| first_indexed | 2024-03-11T18:42:04Z |
| format | Article |
| id | doaj.art-c0a8ae77d91040f0a50ef562f3dbe71a |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-03-11T18:42:04Z |
| publishDate | 2020-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-c0a8ae77d91040f0a50ef562f3dbe71a2023-10-12T12:06:11ZengWileyFood Science & Nutrition2048-71772020-08-01884591460010.1002/fsn3.1776The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathwayGuofu Yi0He Li1You Li2Fen Zhao3Zhiwei Ying4Menglan Liu5Jian Zhang6Xinqi Liu7Beijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Engineering and Technology Research Center of Food Additives Beijing Technology and Business University (BTBU) Beijing ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Engineering and Technology Research Center of Food Additives Beijing Technology and Business University (BTBU) Beijing ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Engineering and Technology Research Center of Food Additives Beijing Technology and Business University (BTBU) Beijing ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Engineering and Technology Research Center of Food Additives Beijing Technology and Business University (BTBU) Beijing ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Engineering and Technology Research Center of Food Additives Beijing Technology and Business University (BTBU) Beijing ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Engineering and Technology Research Center of Food Additives Beijing Technology and Business University (BTBU) Beijing ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Engineering and Technology Research Center of Food Additives Beijing Technology and Business University (BTBU) Beijing ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Engineering and Technology Research Center of Food Additives Beijing Technology and Business University (BTBU) Beijing ChinaAbstract Soybean protein‐derived peptides (SBP) are a rich source of various bioactive peptides with multiple health benefits. However, the prospective effects of SBP on human cells are still unclear. Therefore, this article investigated the effects of small molecular weight SBP on MG132‐induced apoptosis in RAW264.7 cells. SBP inhibited MG132‐induced apoptosis of RAW264.7 cells in a dose‐dependent manner by flow cytometry. To further study its molecular mechanisms, Western blot analysis demonstrated that SBP could activate the PI3K‐AKT pathway by increasing the phosphorylation of PI3K and AKT and inhibiting apoptosis pathway by downregulating the expressions of pro‐apoptotic proteins of Bim, Bax, Fas, and Fasl and promoting the expressions of anti‐apoptotic proteins of Bcl‐xL and Bcl‐2. These results indicated the protective effect of SBP on MG132‐induced apoptosis in RAW264.7 cells.https://doi.org/10.1002/fsn3.1776apoptosis pathwayMG132PI3K‐AKT pathwaysoybean protein‐derived peptides |
| spellingShingle | Guofu Yi He Li You Li Fen Zhao Zhiwei Ying Menglan Liu Jian Zhang Xinqi Liu The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway Food Science & Nutrition apoptosis pathway MG132 PI3K‐AKT pathway soybean protein‐derived peptides |
| title | The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway |
| title_full | The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway |
| title_fullStr | The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway |
| title_full_unstemmed | The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway |
| title_short | The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway |
| title_sort | protective effect of soybean protein derived peptides on apoptosis via the activation of pi3k akt and inhibition on apoptosis pathway |
| topic | apoptosis pathway MG132 PI3K‐AKT pathway soybean protein‐derived peptides |
| url | https://doi.org/10.1002/fsn3.1776 |
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