Pathogenetic role of Staphylococcus aureus in purulent keratoconjunctivitis in cats
The research was carried out in the Department of Surgery and Obstetrics of Agricultural Animals of Dnipro State Agrarian and Economic University on clinically healthy outbred cats of different ages with purulent keratoconjunctivitis. Hematological, biochemical and immunological parameters were dete...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Oles Honchar Dnipro National University
2019-11-01
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| Series: | Regulatory Mechanisms in Biosystems |
| Subjects: | |
| Online Access: | https://medicine.dp.ua/index.php/med/article/view/574 |
| _version_ | 1811333097677914112 |
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| author | S. N. Maslikov D. D. Bely V. V. Samoiliuk V. V. Vakulik T. L. Spitsyna |
| author_facet | S. N. Maslikov D. D. Bely V. V. Samoiliuk V. V. Vakulik T. L. Spitsyna |
| author_sort | S. N. Maslikov |
| collection | DOAJ |
| description | The research was carried out in the Department of Surgery and Obstetrics of Agricultural Animals of Dnipro State Agrarian and Economic University on clinically healthy outbred cats of different ages with purulent keratoconjunctivitis. Hematological, biochemical and immunological parameters were determined in the animals, and microbiological and virological research was conducted on them. According to the data obtained, more than half of cases of ophthalmopathology in cats were conjunctivitis and keratoconjunctivitis, and they were more often registered in the cold season. The main causes of eye diseases in the cats were mechanical injuries, coccal and chlamydial infection, allergy and development of disease against the background of primary lesions of the ears and paranasal sinuses. Among the detected microorganisms, the vast majority (81.9%) were staphylococci, including S. albus, S. aureus and S. epidermidis. All types of microorganisms except O-forms of Bacillus sp. exhibited high and medium sensitivity to antibiotics. Immunoblotting revealed polypeptides that responded to specific S. aureus antigens in samples of conjunctiva, cornea, intraocular fluid, and blood of cats suffering from purulent (staphylococcal) keratoconjunctivitis. The highest antigen concentration was detected in the cornea and conjunctiva. In the absence of expressive shifts of the investigated hematological and biochemical parameters, the dynamics of immunological markers were shown by a significant increase in the content of immunoglobulins and circulating immune complexes, as well as by a distinct activation of the complement system by the classical route. The results showed a clear gradual activation of phagocytosis, namely: the number of phagocytic neutrophils increased, reaching its maximum value by the seventh day of development of purulent keratoconjunctivitis. The phagocytic index in the first three days of observation tended to decrease, and by the seventh day it had already significantly exceeded the initial value. Despite the ambiguous dynamics of the phagocytic index, in the course of the development of the inflammatory process in the blood of sick cats, we observed a clear increase in the index of complete phagocytosis. Thus, the development of purulent keratoconjunctivitis in cats occurs against the background of clear cellular and humoral responses to the infectious agent. |
| first_indexed | 2024-04-13T16:47:42Z |
| format | Article |
| id | doaj.art-c0a9dd98fda44e1b8fc60841a6b6eda6 |
| institution | Directory Open Access Journal |
| issn | 2519-8521 2520-2588 |
| language | English |
| last_indexed | 2024-04-13T16:47:42Z |
| publishDate | 2019-11-01 |
| publisher | Oles Honchar Dnipro National University |
| record_format | Article |
| series | Regulatory Mechanisms in Biosystems |
| spelling | doaj.art-c0a9dd98fda44e1b8fc60841a6b6eda62022-12-22T02:39:02ZengOles Honchar Dnipro National UniversityRegulatory Mechanisms in Biosystems2519-85212520-25882019-11-0110450751210.15421/021974574Pathogenetic role of Staphylococcus aureus in purulent keratoconjunctivitis in catsS. N. Maslikov0D. D. Bely1V. V. Samoiliuk2V. V. Vakulik3T. L. Spitsyna4Dnipro State Agrarian and Economic UniversityDnipro State Agrarian and Economic UniversityDnipro State Agrarian and Economic UniversityDnipro State Agrarian and Economic UniversityDnipro State Agrarian and Economic UniversityThe research was carried out in the Department of Surgery and Obstetrics of Agricultural Animals of Dnipro State Agrarian and Economic University on clinically healthy outbred cats of different ages with purulent keratoconjunctivitis. Hematological, biochemical and immunological parameters were determined in the animals, and microbiological and virological research was conducted on them. According to the data obtained, more than half of cases of ophthalmopathology in cats were conjunctivitis and keratoconjunctivitis, and they were more often registered in the cold season. The main causes of eye diseases in the cats were mechanical injuries, coccal and chlamydial infection, allergy and development of disease against the background of primary lesions of the ears and paranasal sinuses. Among the detected microorganisms, the vast majority (81.9%) were staphylococci, including S. albus, S. aureus and S. epidermidis. All types of microorganisms except O-forms of Bacillus sp. exhibited high and medium sensitivity to antibiotics. Immunoblotting revealed polypeptides that responded to specific S. aureus antigens in samples of conjunctiva, cornea, intraocular fluid, and blood of cats suffering from purulent (staphylococcal) keratoconjunctivitis. The highest antigen concentration was detected in the cornea and conjunctiva. In the absence of expressive shifts of the investigated hematological and biochemical parameters, the dynamics of immunological markers were shown by a significant increase in the content of immunoglobulins and circulating immune complexes, as well as by a distinct activation of the complement system by the classical route. The results showed a clear gradual activation of phagocytosis, namely: the number of phagocytic neutrophils increased, reaching its maximum value by the seventh day of development of purulent keratoconjunctivitis. The phagocytic index in the first three days of observation tended to decrease, and by the seventh day it had already significantly exceeded the initial value. Despite the ambiguous dynamics of the phagocytic index, in the course of the development of the inflammatory process in the blood of sick cats, we observed a clear increase in the index of complete phagocytosis. Thus, the development of purulent keratoconjunctivitis in cats occurs against the background of clear cellular and humoral responses to the infectious agent.https://medicine.dp.ua/index.php/med/article/view/574cats; ophthalmopathology; diagnostics; microorganisms; immunoblotting. |
| spellingShingle | S. N. Maslikov D. D. Bely V. V. Samoiliuk V. V. Vakulik T. L. Spitsyna Pathogenetic role of Staphylococcus aureus in purulent keratoconjunctivitis in cats Regulatory Mechanisms in Biosystems cats; ophthalmopathology; diagnostics; microorganisms; immunoblotting. |
| title | Pathogenetic role of Staphylococcus aureus in purulent keratoconjunctivitis in cats |
| title_full | Pathogenetic role of Staphylococcus aureus in purulent keratoconjunctivitis in cats |
| title_fullStr | Pathogenetic role of Staphylococcus aureus in purulent keratoconjunctivitis in cats |
| title_full_unstemmed | Pathogenetic role of Staphylococcus aureus in purulent keratoconjunctivitis in cats |
| title_short | Pathogenetic role of Staphylococcus aureus in purulent keratoconjunctivitis in cats |
| title_sort | pathogenetic role of staphylococcus aureus in purulent keratoconjunctivitis in cats |
| topic | cats; ophthalmopathology; diagnostics; microorganisms; immunoblotting. |
| url | https://medicine.dp.ua/index.php/med/article/view/574 |
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