Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill.)
Micropropagation is considered a successful alternative for aloe (Aloe barbadensis Mill.) plant production. However, it has limited use due to the high production cost. Liquid media were compared to agar-gelled medium during all micropropagation stages of aloe to reduce the cost for gelling agent us...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Spanish |
| Published: |
Universidad Nacional de Colombia
2015-01-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | https://revistas.unal.edu.co/index.php/biotecnologia/article/view/50669 |
| _version_ | 1811217528992563200 |
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| author | Nilca Rosa Albany Jorge Alberto Vilchez Silvia León Alba Ruth Nava Leonardo Javier Martínez Miguel Angel Molina |
| author_facet | Nilca Rosa Albany Jorge Alberto Vilchez Silvia León Alba Ruth Nava Leonardo Javier Martínez Miguel Angel Molina |
| author_sort | Nilca Rosa Albany |
| collection | DOAJ |
| description | Micropropagation is considered a successful alternative for aloe (Aloe barbadensis Mill.) plant production. However, it has limited use due to the high production cost. Liquid media were compared to agar-gelled medium during all micropropagation stages of aloe to reduce the cost for gelling agent used. In the establishment stage, there was a higher percentage of contaminated explants in static liquid medium (25.55%) than those cultured in agar-gelled medium (11.11%), although all the explants were established independently of the culture medium used, higher height (3.81 cm) was observed in liquid medium than those growing in agar-gelled medium (3.03 cm). In the multiplication stage, explant height was higher in the recipients used for automated temporary immersion system (RITA®) (4.43 - 6.01 cm) than those cultured in agar-gelled medium (3.24 - 3.42 cm), there was no significant difference for number of shoots/explant. All shoots had roots at 30 days independently of used culture media (static liquid or agar-gelled media). Shoot height, number and root length had similar values in both culture media. The implementation of liquid media and automated temporary Immersion system RITA® may allow to reduce production costs of gelling agent used, it represents an approach for the commercial micropropagation of aloe |
| first_indexed | 2024-04-12T06:56:18Z |
| format | Article |
| id | doaj.art-c0aa9460b150491fab7480ff9046cf3e |
| institution | Directory Open Access Journal |
| issn | 0123-3475 1909-8758 |
| language | Spanish |
| last_indexed | 2024-04-12T06:56:18Z |
| publishDate | 2015-01-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Biotecnología |
| spelling | doaj.art-c0aa9460b150491fab7480ff9046cf3e2022-12-22T03:43:09ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582015-01-01171243110.15446/rev.colomb.biote.v17n1.5066940046Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill.)Nilca Rosa Albany0Jorge Alberto Vilchez1Silvia León2Alba Ruth Nava3Leonardo Javier Martínez4Miguel Angel Molina5Universidad del ZuliaUniversidad del ZuliaUniversidad del ZuliaUniversidad del ZuliaUniversidad del ZuliaUniversidad del ZuliaMicropropagation is considered a successful alternative for aloe (Aloe barbadensis Mill.) plant production. However, it has limited use due to the high production cost. Liquid media were compared to agar-gelled medium during all micropropagation stages of aloe to reduce the cost for gelling agent used. In the establishment stage, there was a higher percentage of contaminated explants in static liquid medium (25.55%) than those cultured in agar-gelled medium (11.11%), although all the explants were established independently of the culture medium used, higher height (3.81 cm) was observed in liquid medium than those growing in agar-gelled medium (3.03 cm). In the multiplication stage, explant height was higher in the recipients used for automated temporary immersion system (RITA®) (4.43 - 6.01 cm) than those cultured in agar-gelled medium (3.24 - 3.42 cm), there was no significant difference for number of shoots/explant. All shoots had roots at 30 days independently of used culture media (static liquid or agar-gelled media). Shoot height, number and root length had similar values in both culture media. The implementation of liquid media and automated temporary Immersion system RITA® may allow to reduce production costs of gelling agent used, it represents an approach for the commercial micropropagation of aloehttps://revistas.unal.edu.co/index.php/biotecnologia/article/view/50669Cultivo de tejidosagentes gelificantesRITA®sistemas de inmersión temporal. |
| spellingShingle | Nilca Rosa Albany Jorge Alberto Vilchez Silvia León Alba Ruth Nava Leonardo Javier Martínez Miguel Angel Molina Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill.) Revista Colombiana de Biotecnología Cultivo de tejidos agentes gelificantes RITA® sistemas de inmersión temporal. |
| title | Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill.) |
| title_full | Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill.) |
| title_fullStr | Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill.) |
| title_full_unstemmed | Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill.) |
| title_short | Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill.) |
| title_sort | liquid medium culture an approach for the commercial micropropagation of aloe aloe barbadensis mill |
| topic | Cultivo de tejidos agentes gelificantes RITA® sistemas de inmersión temporal. |
| url | https://revistas.unal.edu.co/index.php/biotecnologia/article/view/50669 |
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