An optimized protocol to detect protein ubiquitination and activation by ubiquitination assay in vivo and CCK-8 assay

An optimized protocol to detect protein ubiquitination and activation by ubiquitination assay in vivo and CCK-8 assay

Summary: E3 ubiquitin ligases play a role in protein degradation, cellular localization, and activation, and their dysregulation is associated with human diseases. Here, we present a protocol to detect IGF2BP1 ubiquitination and activation by an E3 ubiquitin ligase FBXO45. We describe steps for prep...

Full description

Bibliographic Details
Main Authors: Xiao-Tong Lin, Jie Zhang, Chuan-Ming Xie
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell Culture
Cell-based Assays
Molecular Biology
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001570

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723001570

Similar Items