Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic Mineralization
Abstract Although deoxyribonucleic acid (DNA) is the genetic coding for the very essence of life, these macromolecules or components thereof are not necessarily lost after a cell dies. There appears to be a link between extracellular DNA and biomineralization. Here the authors demonstrate that extra...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-02-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202103693 |
| _version_ | 1819278423834492928 |
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| author | Min‐juan Shen Kai Jiao Chen‐yu Wang Hermann Ehrlich Mei‐chen Wan Dong‐xiao Hao Jing Li Qian‐qian Wan Lige Tonggu Jian‐fei Yan Kai‐yan Wang Yu‐xuan Ma Ji‐hua Chen Franklin R. Tay Li‐na Niu |
| author_facet | Min‐juan Shen Kai Jiao Chen‐yu Wang Hermann Ehrlich Mei‐chen Wan Dong‐xiao Hao Jing Li Qian‐qian Wan Lige Tonggu Jian‐fei Yan Kai‐yan Wang Yu‐xuan Ma Ji‐hua Chen Franklin R. Tay Li‐na Niu |
| author_sort | Min‐juan Shen |
| collection | DOAJ |
| description | Abstract Although deoxyribonucleic acid (DNA) is the genetic coding for the very essence of life, these macromolecules or components thereof are not necessarily lost after a cell dies. There appears to be a link between extracellular DNA and biomineralization. Here the authors demonstrate that extracellular DNA functions as an initiator of collagen intrafibrillar mineralization. This is confirmed with in vitro and in vivo biological mineralization models. Because of their polyanionic property, extracellular DNA molecules are capable of stabilizing supersaturated calcium phosphate solution and mineralizing 2D and 3D collagen matrices completely as early as 24 h. The effectiveness of extracellular DNA in biomineralization of collagen is attributed to the relatively stable formation of amorphous liquid droplets triggered by attraction of DNA to the collagen fibrils via hydrogen bonding. These findings suggest that extracellular DNA is biomimetically significant for fabricating inorganic–organic hybrid materials for tissue engineering. DNA‐induced collagen intrafibrillar mineralization provides a clue to the pathogenesis of ectopic mineralization in different body tissues. The use of DNase for targeting extracellular DNA at destined tissue sites provides a potential solution for treatment of diseases associated with ectopic mineralization. |
| first_indexed | 2024-12-24T00:11:47Z |
| format | Article |
| id | doaj.art-c0af4d0dbd5b4cbd9e24499abcdca9b0 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-12-24T00:11:47Z |
| publishDate | 2022-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-c0af4d0dbd5b4cbd9e24499abcdca9b02022-12-21T17:24:52ZengWileyAdvanced Science2198-38442022-02-0195n/an/a10.1002/advs.202103693Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic MineralizationMin‐juan Shen0Kai Jiao1Chen‐yu Wang2Hermann Ehrlich3Mei‐chen Wan4Dong‐xiao Hao5Jing Li6Qian‐qian Wan7Lige Tonggu8Jian‐fei Yan9Kai‐yan Wang10Yu‐xuan Ma11Ji‐hua Chen12Franklin R. Tay13Li‐na Niu14National Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaNational Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaNational Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaInstitute of Electronic and Sensor Materials TU Bergakademie Freiberg Freiberg 09599 GermanyNational Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaNational Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaNational Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaNational Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaSchool of Medicine University of Washington Seattle WA 98195 USANational Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaNational Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaNational Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaNational Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaThe Dental College of Georgia Augusta University Augusta GA 30912 USANational Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Stomatology, School of Stomatology The Fourth Military Medical University Xi'an Shaanxi 710032 P. R. ChinaAbstract Although deoxyribonucleic acid (DNA) is the genetic coding for the very essence of life, these macromolecules or components thereof are not necessarily lost after a cell dies. There appears to be a link between extracellular DNA and biomineralization. Here the authors demonstrate that extracellular DNA functions as an initiator of collagen intrafibrillar mineralization. This is confirmed with in vitro and in vivo biological mineralization models. Because of their polyanionic property, extracellular DNA molecules are capable of stabilizing supersaturated calcium phosphate solution and mineralizing 2D and 3D collagen matrices completely as early as 24 h. The effectiveness of extracellular DNA in biomineralization of collagen is attributed to the relatively stable formation of amorphous liquid droplets triggered by attraction of DNA to the collagen fibrils via hydrogen bonding. These findings suggest that extracellular DNA is biomimetically significant for fabricating inorganic–organic hybrid materials for tissue engineering. DNA‐induced collagen intrafibrillar mineralization provides a clue to the pathogenesis of ectopic mineralization in different body tissues. The use of DNase for targeting extracellular DNA at destined tissue sites provides a potential solution for treatment of diseases associated with ectopic mineralization.https://doi.org/10.1002/advs.202103693amorphous calcium phosphatebiomineralizationcollagenectopic calcificationextracellular nucleic acids |
| spellingShingle | Min‐juan Shen Kai Jiao Chen‐yu Wang Hermann Ehrlich Mei‐chen Wan Dong‐xiao Hao Jing Li Qian‐qian Wan Lige Tonggu Jian‐fei Yan Kai‐yan Wang Yu‐xuan Ma Ji‐hua Chen Franklin R. Tay Li‐na Niu Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic Mineralization Advanced Science amorphous calcium phosphate biomineralization collagen ectopic calcification extracellular nucleic acids |
| title | Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic Mineralization |
| title_full | Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic Mineralization |
| title_fullStr | Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic Mineralization |
| title_full_unstemmed | Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic Mineralization |
| title_short | Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic Mineralization |
| title_sort | extracellular dna a missing link in the pathogenesis of ectopic mineralization |
| topic | amorphous calcium phosphate biomineralization collagen ectopic calcification extracellular nucleic acids |
| url | https://doi.org/10.1002/advs.202103693 |
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