High Genetic Diversity of HIV-1 and Active Transmission Clusters among Male-to-Male Sexual Contacts (MMSCs) in Zhuhai, China
Monitoring genetic diversity and recent HIV infections (RHIs) is critical for understanding HIV epidemiology. Here, we report HIV-1 genetic diversity and RHIs in blood samples from 190 HIV-positive MMSCs in Zhuhai, China. MMSCs with newly reported HIV were enrolled from January 2020 to June 2022. A...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/9/1947 |
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| author | Yi Zhou Mingting Cui Zhongsi Hong Shaoli Huang Shuntai Zhou Hang Lyu Jiarun Li Yixiong Lin Huitao Huang Weiming Tang Caijun Sun Wenyan Huang |
| author_facet | Yi Zhou Mingting Cui Zhongsi Hong Shaoli Huang Shuntai Zhou Hang Lyu Jiarun Li Yixiong Lin Huitao Huang Weiming Tang Caijun Sun Wenyan Huang |
| author_sort | Yi Zhou |
| collection | DOAJ |
| description | Monitoring genetic diversity and recent HIV infections (RHIs) is critical for understanding HIV epidemiology. Here, we report HIV-1 genetic diversity and RHIs in blood samples from 190 HIV-positive MMSCs in Zhuhai, China. MMSCs with newly reported HIV were enrolled from January 2020 to June 2022. A nested PCR was performed to amplify the HIV polymerase gene fragments at HXB2 positions 2604–3606. We constructed genetic transmission network at both 0.5% and 1.5% distance thresholds using the Tamura-Nei93 model. RHIs were identified using a recent infection testing algorithm (RITA) combining limiting antigen avidity enzyme immunoassay (LAg-EIA) assay with clinical data. The results revealed that 19.5% (37/190) were RHIs and 48.4% (92/190) were CRF07_BC. Two clusters were identified at a 0.5% distance threshold. Among them, one was infected with CRF07_BC for the long term, and the other was infected with CRF55_01B recently. We identified a total of 15 clusters at a 1.5% distance threshold. Among them, nine were infected with CRF07_BC subtype, and RHIs were found in 38.8% (19/49) distributed in eight genetic clusters. We identified a large active transmission cluster (<i>n</i> = 10) infected with a genetic variant, CRF79_0107. The multivariable logistic regression model showed that clusters were more likely to be RHIs (adjusted OR: 3.64, 95% CI: 1.51~9.01). The RHI algorithm can help to identify recent or ongoing transmission clusters where the prevention tools are mostly needed. Prompt public health measures are needed to contain the further spread of active transmission clusters. |
| first_indexed | 2024-03-10T21:51:51Z |
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| id | doaj.art-c0b0828224d54d9a84c7ad2367e5becb |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T21:51:51Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c0b0828224d54d9a84c7ad2367e5becb2023-11-19T13:23:58ZengMDPI AGViruses1999-49152023-09-01159194710.3390/v15091947High Genetic Diversity of HIV-1 and Active Transmission Clusters among Male-to-Male Sexual Contacts (MMSCs) in Zhuhai, ChinaYi Zhou0Mingting Cui1Zhongsi Hong2Shaoli Huang3Shuntai Zhou4Hang Lyu5Jiarun Li6Yixiong Lin7Huitao Huang8Weiming Tang9Caijun Sun10Wenyan Huang11Faculty of Medicine, Macau University of Science and Technology, Macau SAR, ChinaSchool of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaDepartment of Infectious Diseases, Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519001, ChinaSchool of Engineering, The Hong Kong University of Science and Technology, Hong Kong 999077, ChinaLineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USADepartment of HIV Prevention, Zhuhai Center for Disease Control and Prevention, Zhuhai 519060, ChinaDepartment of HIV Prevention, Zhuhai Center for Disease Control and Prevention, Zhuhai 519060, ChinaDepartment of HIV Prevention, Zhuhai Center for Disease Control and Prevention, Zhuhai 519060, ChinaDepartment of HIV Prevention, Zhuhai Center for Disease Control and Prevention, Zhuhai 519060, ChinaDermatology Hospital of Southern Medical University, Guangzhou 510315, ChinaSchool of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaDepartment of HIV Prevention, Zhuhai Center for Disease Control and Prevention, Zhuhai 519060, ChinaMonitoring genetic diversity and recent HIV infections (RHIs) is critical for understanding HIV epidemiology. Here, we report HIV-1 genetic diversity and RHIs in blood samples from 190 HIV-positive MMSCs in Zhuhai, China. MMSCs with newly reported HIV were enrolled from January 2020 to June 2022. A nested PCR was performed to amplify the HIV polymerase gene fragments at HXB2 positions 2604–3606. We constructed genetic transmission network at both 0.5% and 1.5% distance thresholds using the Tamura-Nei93 model. RHIs were identified using a recent infection testing algorithm (RITA) combining limiting antigen avidity enzyme immunoassay (LAg-EIA) assay with clinical data. The results revealed that 19.5% (37/190) were RHIs and 48.4% (92/190) were CRF07_BC. Two clusters were identified at a 0.5% distance threshold. Among them, one was infected with CRF07_BC for the long term, and the other was infected with CRF55_01B recently. We identified a total of 15 clusters at a 1.5% distance threshold. Among them, nine were infected with CRF07_BC subtype, and RHIs were found in 38.8% (19/49) distributed in eight genetic clusters. We identified a large active transmission cluster (<i>n</i> = 10) infected with a genetic variant, CRF79_0107. The multivariable logistic regression model showed that clusters were more likely to be RHIs (adjusted OR: 3.64, 95% CI: 1.51~9.01). The RHI algorithm can help to identify recent or ongoing transmission clusters where the prevention tools are mostly needed. Prompt public health measures are needed to contain the further spread of active transmission clusters.https://www.mdpi.com/1999-4915/15/9/1947human immunodeficiency virusphylogenetic analysistransmission networkrecent HIV infectionrecent infection testing algorithms |
| spellingShingle | Yi Zhou Mingting Cui Zhongsi Hong Shaoli Huang Shuntai Zhou Hang Lyu Jiarun Li Yixiong Lin Huitao Huang Weiming Tang Caijun Sun Wenyan Huang High Genetic Diversity of HIV-1 and Active Transmission Clusters among Male-to-Male Sexual Contacts (MMSCs) in Zhuhai, China Viruses human immunodeficiency virus phylogenetic analysis transmission network recent HIV infection recent infection testing algorithms |
| title | High Genetic Diversity of HIV-1 and Active Transmission Clusters among Male-to-Male Sexual Contacts (MMSCs) in Zhuhai, China |
| title_full | High Genetic Diversity of HIV-1 and Active Transmission Clusters among Male-to-Male Sexual Contacts (MMSCs) in Zhuhai, China |
| title_fullStr | High Genetic Diversity of HIV-1 and Active Transmission Clusters among Male-to-Male Sexual Contacts (MMSCs) in Zhuhai, China |
| title_full_unstemmed | High Genetic Diversity of HIV-1 and Active Transmission Clusters among Male-to-Male Sexual Contacts (MMSCs) in Zhuhai, China |
| title_short | High Genetic Diversity of HIV-1 and Active Transmission Clusters among Male-to-Male Sexual Contacts (MMSCs) in Zhuhai, China |
| title_sort | high genetic diversity of hiv 1 and active transmission clusters among male to male sexual contacts mmscs in zhuhai china |
| topic | human immunodeficiency virus phylogenetic analysis transmission network recent HIV infection recent infection testing algorithms |
| url | https://www.mdpi.com/1999-4915/15/9/1947 |
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