Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study
Intracranial bacterial infection remains a major cause of morbidity and mortality in neurosurgical cases. Metabolomic profiling of cerebrospinal fluid (CSF) holds great promise to gain insights into the pathogenesis of central neural system (CNS) bacterial infections. In this pilot study, we analyze...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/22/6871 |
| _version_ | 1797509177883492352 |
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| author | Yiwen Wang Yu Liu Ruoping Chen Liang Qiao |
| author_facet | Yiwen Wang Yu Liu Ruoping Chen Liang Qiao |
| author_sort | Yiwen Wang |
| collection | DOAJ |
| description | Intracranial bacterial infection remains a major cause of morbidity and mortality in neurosurgical cases. Metabolomic profiling of cerebrospinal fluid (CSF) holds great promise to gain insights into the pathogenesis of central neural system (CNS) bacterial infections. In this pilot study, we analyzed the metabolites in CSF of CNS infection patients and controls in a pseudo-targeted manner, aiming at elucidating the metabolic dysregulation in response to postoperative intracranial bacterial infection of pediatric cases. Untargeted analysis uncovered 597 metabolites, and screened out 206 differential metabolites in case of infection. Targeted verification and pathway analysis filtered out the glycolysis, amino acids metabolism and purine metabolism pathways as potential pathological pathways. These perturbed pathways are involved in the infection-induced oxidative stress and immune response. Characterization of the infection-induced metabolic changes can provide robust biomarkers of CNS bacterial infection for clinical diagnosis, novel pathways for pathological investigation, and new targets for treatment. |
| first_indexed | 2024-03-10T05:14:16Z |
| format | Article |
| id | doaj.art-c0b2d4d0b8c94ff8ae15232b345a772e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:14:16Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c0b2d4d0b8c94ff8ae15232b345a772e2023-11-23T00:35:00ZengMDPI AGMolecules1420-30492021-11-012622687110.3390/molecules26226871Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot StudyYiwen Wang0Yu Liu1Ruoping Chen2Liang Qiao3Department of Chemistry, Shanghai Stomatological Hospital, Fudan University, Shanghai 200000, ChinaDepartment of Neurosurgery, Shanghai Children’s Hospital, Shanghai Jiao Tong University, Shanghai 200062, ChinaDepartment of Pediatric Neurosurgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, ChinaDepartment of Chemistry, Shanghai Stomatological Hospital, Fudan University, Shanghai 200000, ChinaIntracranial bacterial infection remains a major cause of morbidity and mortality in neurosurgical cases. Metabolomic profiling of cerebrospinal fluid (CSF) holds great promise to gain insights into the pathogenesis of central neural system (CNS) bacterial infections. In this pilot study, we analyzed the metabolites in CSF of CNS infection patients and controls in a pseudo-targeted manner, aiming at elucidating the metabolic dysregulation in response to postoperative intracranial bacterial infection of pediatric cases. Untargeted analysis uncovered 597 metabolites, and screened out 206 differential metabolites in case of infection. Targeted verification and pathway analysis filtered out the glycolysis, amino acids metabolism and purine metabolism pathways as potential pathological pathways. These perturbed pathways are involved in the infection-induced oxidative stress and immune response. Characterization of the infection-induced metabolic changes can provide robust biomarkers of CNS bacterial infection for clinical diagnosis, novel pathways for pathological investigation, and new targets for treatment.https://www.mdpi.com/1420-3049/26/22/6871cerebrospinal fluidcentral neural system infectionmass spectrometrymetabolomicsmetabolic pathway |
| spellingShingle | Yiwen Wang Yu Liu Ruoping Chen Liang Qiao Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study Molecules cerebrospinal fluid central neural system infection mass spectrometry metabolomics metabolic pathway |
| title | Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study |
| title_full | Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study |
| title_fullStr | Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study |
| title_full_unstemmed | Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study |
| title_short | Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study |
| title_sort | metabolomic characterization of cerebrospinal fluid from intracranial bacterial infection pediatric patients a pilot study |
| topic | cerebrospinal fluid central neural system infection mass spectrometry metabolomics metabolic pathway |
| url | https://www.mdpi.com/1420-3049/26/22/6871 |
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