Genome-wide identification and expression analysis of WRKY gene family in Capsicum annuum L.
The WRKY family of transcription factors is one of the most important families of plant transcriptional regulators with members regulating multiple biological processes, especially in regulating defense against biotic and abiotic stresses. However, little information is available about WRKYs in pepp...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00211/full |
| _version_ | 1811307781001576448 |
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| author | Weiping eDiao Weiping eDiao John C eSnyder Shubin eWang Jinbing eLiu Baogui ePan Guangjun eGuo Wei eGe |
| author_facet | Weiping eDiao Weiping eDiao John C eSnyder Shubin eWang Jinbing eLiu Baogui ePan Guangjun eGuo Wei eGe |
| author_sort | Weiping eDiao |
| collection | DOAJ |
| description | The WRKY family of transcription factors is one of the most important families of plant transcriptional regulators with members regulating multiple biological processes, especially in regulating defense against biotic and abiotic stresses. However, little information is available about WRKYs in pepper (Capsicum annuum L.). The recent release of completely assembled genome sequences of pepper allowed us to perform a genome-wide investigation for pepper WRKY proteins. In the present study, a total of 71 WRKY genes were identified in the pepper genome. According to structural features of their encoded proteins, the pepper WRKY genes (CaWRKY) were classified into three main groups, with the second group further divided into five subgroups. Genome mapping analysis revealed that CaWRKY were enriched on four chromosomes, especially on chromosome 1, and 15.5% of the family members were tandemly duplicated genes. A phylogenetic tree was constructed depending on WRKY domain’ sequences derived from pepper and Arabidopsis. The expression of 21 selected CaWRKY genes in response to seven different biotic and abiotic stresses (salt, heat shock, drought, Phytophtora capsici, SA, MeJA and ABA) was evaluated by quantitative RT-PCR; Some CaWRKYs were highly expressed and up-regulated by stress treatment. Our results will provide a platform for functional identification and molecular breeding studies of WRKY genes in pepper. |
| first_indexed | 2024-04-13T09:10:18Z |
| format | Article |
| id | doaj.art-c0b4642db8434508a3b80e343bd1e9c5 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T09:10:18Z |
| publishDate | 2016-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c0b4642db8434508a3b80e343bd1e9c52022-12-22T02:52:53ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-02-01710.3389/fpls.2016.00211172497Genome-wide identification and expression analysis of WRKY gene family in Capsicum annuum L.Weiping eDiao0Weiping eDiao1John C eSnyder2Shubin eWang3Jinbing eLiu4Baogui ePan5Guangjun eGuo6Wei eGe7Jiangsu Academy of Agricultural SciencesUniversity of KentuckyUniversity of KentuckyJiangsu Academy of Agricultural SciencesJiangsu Academy of Agricultural SciencesJiangsu Academy of Agricultural SciencesJiangsu Academy of Agricultural SciencesJiangsu Academy of Agricultural SciencesThe WRKY family of transcription factors is one of the most important families of plant transcriptional regulators with members regulating multiple biological processes, especially in regulating defense against biotic and abiotic stresses. However, little information is available about WRKYs in pepper (Capsicum annuum L.). The recent release of completely assembled genome sequences of pepper allowed us to perform a genome-wide investigation for pepper WRKY proteins. In the present study, a total of 71 WRKY genes were identified in the pepper genome. According to structural features of their encoded proteins, the pepper WRKY genes (CaWRKY) were classified into three main groups, with the second group further divided into five subgroups. Genome mapping analysis revealed that CaWRKY were enriched on four chromosomes, especially on chromosome 1, and 15.5% of the family members were tandemly duplicated genes. A phylogenetic tree was constructed depending on WRKY domain’ sequences derived from pepper and Arabidopsis. The expression of 21 selected CaWRKY genes in response to seven different biotic and abiotic stresses (salt, heat shock, drought, Phytophtora capsici, SA, MeJA and ABA) was evaluated by quantitative RT-PCR; Some CaWRKYs were highly expressed and up-regulated by stress treatment. Our results will provide a platform for functional identification and molecular breeding studies of WRKY genes in pepper.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00211/fullTranscription Factorspepperphylogenetic analysisWRKYExpression |
| spellingShingle | Weiping eDiao Weiping eDiao John C eSnyder Shubin eWang Jinbing eLiu Baogui ePan Guangjun eGuo Wei eGe Genome-wide identification and expression analysis of WRKY gene family in Capsicum annuum L. Frontiers in Plant Science Transcription Factors pepper phylogenetic analysis WRKY Expression |
| title | Genome-wide identification and expression analysis of WRKY gene family in Capsicum annuum L. |
| title_full | Genome-wide identification and expression analysis of WRKY gene family in Capsicum annuum L. |
| title_fullStr | Genome-wide identification and expression analysis of WRKY gene family in Capsicum annuum L. |
| title_full_unstemmed | Genome-wide identification and expression analysis of WRKY gene family in Capsicum annuum L. |
| title_short | Genome-wide identification and expression analysis of WRKY gene family in Capsicum annuum L. |
| title_sort | genome wide identification and expression analysis of wrky gene family in capsicum annuum l |
| topic | Transcription Factors pepper phylogenetic analysis WRKY Expression |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00211/full |
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