Enzyme-Assisted Extraction of Oil from Wet Microalgae Scenedesmus sp. G4
The enzyme-assisted aqueous extraction of oil from wet microalgae was employed to avoid the energy consumption of a dewatering process. In this paper, oil-rich microalgae Scenedesmus sp. G4 was hydrolyzed by enzyme mixtures for oil extraction. The results showed that the algae concentration had the...
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MDPI AG
2015-08-01
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| Series: | Energies |
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| Online Access: | http://www.mdpi.com/1996-1073/8/8/8165 |
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| author | Shuhao Huo Zhongming Wang Fengjie Cui Bin Zou Pengxiang Zhao Zhenhong Yuan |
| author_facet | Shuhao Huo Zhongming Wang Fengjie Cui Bin Zou Pengxiang Zhao Zhenhong Yuan |
| author_sort | Shuhao Huo |
| collection | DOAJ |
| description | The enzyme-assisted aqueous extraction of oil from wet microalgae was employed to avoid the energy consumption of a dewatering process. In this paper, oil-rich microalgae Scenedesmus sp. G4 was hydrolyzed by enzyme mixtures for oil extraction. The results showed that the algae concentration had the greatest influence on yield of extracted oil, and the temperature and the ratio of enzyme mixtures affected the results as follows: the maximum yield of oil extracted from Scenedesmus sp. G4 reached 86.1% under optimal conditions with an algae concentration at 2.5 g/L, temperature 30/50 °C, pH 3.5/4.5 and a cellulase:pectinase:hemicellulase ratio of 1:1:1 or 1:2:1 (w/w/w). The results also indicated that the enzyme mixtures had a significant impact on the integrity of microalgae cells and the crystallization index increased from 30.7% to around 36.0% after enzymatic hydrolysis treatment. The cell wall of Scenedesmus sp. G4 has a high content of cellulose Iα and low crystallization, which is beneficial to the oil extraction by the enzyme-assisted hydrolysis method. |
| first_indexed | 2024-04-11T12:52:10Z |
| format | Article |
| id | doaj.art-c0b712a5cb194214acfefb8e919470fb |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-04-11T12:52:10Z |
| publishDate | 2015-08-01 |
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| series | Energies |
| spelling | doaj.art-c0b712a5cb194214acfefb8e919470fb2022-12-22T04:23:11ZengMDPI AGEnergies1996-10732015-08-01888165817410.3390/en8088165en8088165Enzyme-Assisted Extraction of Oil from Wet Microalgae Scenedesmus sp. G4Shuhao Huo0Zhongming Wang1Fengjie Cui2Bin Zou3Pengxiang Zhao4Zhenhong Yuan5School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, ChinaKey Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, Guangdong, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, ChinaBeijing Biomass Energy Technology Center, State Grid Energy Conservation Service Ltd., Beijing 100053, ChinaKey Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, Guangdong, ChinaThe enzyme-assisted aqueous extraction of oil from wet microalgae was employed to avoid the energy consumption of a dewatering process. In this paper, oil-rich microalgae Scenedesmus sp. G4 was hydrolyzed by enzyme mixtures for oil extraction. The results showed that the algae concentration had the greatest influence on yield of extracted oil, and the temperature and the ratio of enzyme mixtures affected the results as follows: the maximum yield of oil extracted from Scenedesmus sp. G4 reached 86.1% under optimal conditions with an algae concentration at 2.5 g/L, temperature 30/50 °C, pH 3.5/4.5 and a cellulase:pectinase:hemicellulase ratio of 1:1:1 or 1:2:1 (w/w/w). The results also indicated that the enzyme mixtures had a significant impact on the integrity of microalgae cells and the crystallization index increased from 30.7% to around 36.0% after enzymatic hydrolysis treatment. The cell wall of Scenedesmus sp. G4 has a high content of cellulose Iα and low crystallization, which is beneficial to the oil extraction by the enzyme-assisted hydrolysis method.http://www.mdpi.com/1996-1073/8/8/8165Scenedesmus sp.wet microalgaeoil extractionenzyme-assisted hydrolysis |
| spellingShingle | Shuhao Huo Zhongming Wang Fengjie Cui Bin Zou Pengxiang Zhao Zhenhong Yuan Enzyme-Assisted Extraction of Oil from Wet Microalgae Scenedesmus sp. G4 Energies Scenedesmus sp. wet microalgae oil extraction enzyme-assisted hydrolysis |
| title | Enzyme-Assisted Extraction of Oil from Wet Microalgae Scenedesmus sp. G4 |
| title_full | Enzyme-Assisted Extraction of Oil from Wet Microalgae Scenedesmus sp. G4 |
| title_fullStr | Enzyme-Assisted Extraction of Oil from Wet Microalgae Scenedesmus sp. G4 |
| title_full_unstemmed | Enzyme-Assisted Extraction of Oil from Wet Microalgae Scenedesmus sp. G4 |
| title_short | Enzyme-Assisted Extraction of Oil from Wet Microalgae Scenedesmus sp. G4 |
| title_sort | enzyme assisted extraction of oil from wet microalgae scenedesmus sp g4 |
| topic | Scenedesmus sp. wet microalgae oil extraction enzyme-assisted hydrolysis |
| url | http://www.mdpi.com/1996-1073/8/8/8165 |
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