Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
BACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylatio...
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| Format: | Article |
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BMC
2015-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050&lng=en&tlng=en |
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| author | Fabiana Greyce Oliveira Almeida Priscila Ferreira de Aquino Afonso Duarte Leäo de Souza Antonia Queiroz Lima de Souza Sonia do Carmo Vinhote Thais Messias Mac-Cormick Marcelo Soares da Mota Silva Sidney Raimundo Silva Chalub Juliana de Saldanha da Gama Fischer Paulo Costa Carvalho Maria da Gloria da Costa Carvalho |
| author_facet | Fabiana Greyce Oliveira Almeida Priscila Ferreira de Aquino Afonso Duarte Leäo de Souza Antonia Queiroz Lima de Souza Sonia do Carmo Vinhote Thais Messias Mac-Cormick Marcelo Soares da Mota Silva Sidney Raimundo Silva Chalub Juliana de Saldanha da Gama Fischer Paulo Costa Carvalho Maria da Gloria da Costa Carvalho |
| author_sort | Fabiana Greyce Oliveira Almeida |
| collection | DOAJ |
| description | BACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world. RESULTS: Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2 genes, respectively. The margin biopsies were methylated in 3/7 (43 %), 2/7 (28 %), 7/7 (100 %), and 6/7 (86 %) for CDH1, CDKN2A, DAPK, and TIMP2, respectively. CONCLUSIONS: Our findings showed DAPK and TIMP2 to be methylated in most samples from both tumor tissues and adjacent non-neoplastic margins; thus presenting distinct methylation patterns. This emphasizes the importance of better understanding of the relation of these patterns with cancer in the context of different populations. |
| first_indexed | 2024-12-21T11:01:24Z |
| format | Article |
| id | doaj.art-c0b9052884d442a8a12dbd5fb47395d0 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 |
| language | English |
| last_indexed | 2024-12-21T11:01:24Z |
| publishDate | 2015-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-c0b9052884d442a8a12dbd5fb47395d02022-12-21T19:06:21ZengBMCBiological Research0716-97602015-01-014801610.1186/S40659-015-0042-7S0716-97602015000100050Colorectal cancer DNA methylation patterns from patients in Manaus, BrazilFabiana Greyce Oliveira Almeida0Priscila Ferreira de Aquino1Afonso Duarte Leäo de Souza2Antonia Queiroz Lima de Souza3Sonia do Carmo Vinhote4Thais Messias Mac-Cormick5Marcelo Soares da Mota Silva6Sidney Raimundo Silva Chalub7Juliana de Saldanha da Gama Fischer8Paulo Costa Carvalho9Maria da Gloria da Costa Carvalho10Federal University of AmazonasFIOCRUZFederal University of AmazonasFederal University of AmazonasAmazon State UniversityFederal University of Rio de JaneiroFederal University of Rio de JaneiroOncology Control Center Foundation of Amazon StateCarlos Chagas InstituteCarlos Chagas InstituteFederal University of Rio de JaneiroBACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world. RESULTS: Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2 genes, respectively. The margin biopsies were methylated in 3/7 (43 %), 2/7 (28 %), 7/7 (100 %), and 6/7 (86 %) for CDH1, CDKN2A, DAPK, and TIMP2, respectively. CONCLUSIONS: Our findings showed DAPK and TIMP2 to be methylated in most samples from both tumor tissues and adjacent non-neoplastic margins; thus presenting distinct methylation patterns. This emphasizes the importance of better understanding of the relation of these patterns with cancer in the context of different populations.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050&lng=en&tlng=enMethylationColorectal cancerAmazonEpigenetic |
| spellingShingle | Fabiana Greyce Oliveira Almeida Priscila Ferreira de Aquino Afonso Duarte Leäo de Souza Antonia Queiroz Lima de Souza Sonia do Carmo Vinhote Thais Messias Mac-Cormick Marcelo Soares da Mota Silva Sidney Raimundo Silva Chalub Juliana de Saldanha da Gama Fischer Paulo Costa Carvalho Maria da Gloria da Costa Carvalho Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil Biological Research Methylation Colorectal cancer Amazon Epigenetic |
| title | Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| title_full | Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| title_fullStr | Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| title_full_unstemmed | Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| title_short | Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| title_sort | colorectal cancer dna methylation patterns from patients in manaus brazil |
| topic | Methylation Colorectal cancer Amazon Epigenetic |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050&lng=en&tlng=en |
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