Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells
Abstract Background Titanium surfaces have been modified by various approaches with the aim of improving the stimulation of osseointegration. Laser beam (Yb-YAG) treatment is a controllable and flexible approach to modifying surfaces. It creates a complex surface topography with micro and nano-scale...
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| Format: | Article |
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BMC
2017-11-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | http://link.springer.com/article/10.1186/s13287-017-0717-9 |
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| author | Tatiana A. B. Bressel Jana Dara Freires de Queiroz Susana Margarida Gomes Moreira Jéssyca T. da Fonseca Edson A. Filho Antônio Carlos Guastaldi Silvia Regina Batistuzzo de Medeiros |
| author_facet | Tatiana A. B. Bressel Jana Dara Freires de Queiroz Susana Margarida Gomes Moreira Jéssyca T. da Fonseca Edson A. Filho Antônio Carlos Guastaldi Silvia Regina Batistuzzo de Medeiros |
| author_sort | Tatiana A. B. Bressel |
| collection | DOAJ |
| description | Abstract Background Titanium surfaces have been modified by various approaches with the aim of improving the stimulation of osseointegration. Laser beam (Yb-YAG) treatment is a controllable and flexible approach to modifying surfaces. It creates a complex surface topography with micro and nano-scaled patterns, and an oxide layer that can improve the osseointegration of implants, increasing their usefulness as bone implant materials. Methods Laser beam irradiation at various fluences (132, 210, or 235 J/cm2) was used to treat commercially pure titanium discs to create complex surface topographies. The titanium discs were investigated by scanning electron microscopy, X-ray diffraction, and measurement of contact angles. The surface generated at a fluence of 235 J/cm2 was used in the biological assays. The behavior of mesenchymal stem cells from an umbilical cord vein was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a mineralization assay, and an alkaline phosphatase activity assay and by carrying out a quantitative real-time polymerase chain reaction for osteogenic markers. CHO-k1 cells were also exposed to titanium discs in the MTT assay. Results The best titanium surface was that produced by laser beam irradiation at 235 J/cm2 fluence. Cell proliferation analysis revealed that the CHO-k1 and mesenchymal stem cells behaved differently. The laser-processed titanium surface increased the proliferation of CHO-k1 cells, reduced the proliferation of mesenchymal stem cells, upregulated the expression of the osteogenic markers, and enhanced alkaline phosphatase activity. Conclusions The laser-treated titanium surface modulated cellular behavior depending on the cell type, and stimulated osteogenic differentiation. This evidence supports the potential use of laser-processed titanium surfaces as bone implant materials, and their use in regenerative medicine could promote better outcomes. |
| first_indexed | 2024-12-14T21:56:05Z |
| format | Article |
| id | doaj.art-c0b972c7fa5c4ef59d9d70feafb4ce39 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-14T21:56:05Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
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| series | Stem Cell Research & Therapy |
| spelling | doaj.art-c0b972c7fa5c4ef59d9d70feafb4ce392022-12-21T22:46:08ZengBMCStem Cell Research & Therapy1757-65122017-11-018111110.1186/s13287-017-0717-9Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cellsTatiana A. B. Bressel0Jana Dara Freires de Queiroz1Susana Margarida Gomes Moreira2Jéssyca T. da Fonseca3Edson A. Filho4Antônio Carlos Guastaldi5Silvia Regina Batistuzzo de Medeiros6Departamento de Biologia Celular e Genética, CB—UFRN, Universidade Federal do Rio Grande do NorteDepartamento de Biologia Celular e Genética, CB—UFRN, Universidade Federal do Rio Grande do NorteDepartamento de Biologia Celular e Genética, CB—UFRN, Universidade Federal do Rio Grande do NorteDepartamento de Biologia Celular e Genética, CB—UFRN, Universidade Federal do Rio Grande do NorteDepartamento de Físico-Química, Instituto de Química de Araraquara—UNESPDepartamento de Físico-Química, Instituto de Química de Araraquara—UNESPDepartamento de Biologia Celular e Genética, CB—UFRN, Universidade Federal do Rio Grande do NorteAbstract Background Titanium surfaces have been modified by various approaches with the aim of improving the stimulation of osseointegration. Laser beam (Yb-YAG) treatment is a controllable and flexible approach to modifying surfaces. It creates a complex surface topography with micro and nano-scaled patterns, and an oxide layer that can improve the osseointegration of implants, increasing their usefulness as bone implant materials. Methods Laser beam irradiation at various fluences (132, 210, or 235 J/cm2) was used to treat commercially pure titanium discs to create complex surface topographies. The titanium discs were investigated by scanning electron microscopy, X-ray diffraction, and measurement of contact angles. The surface generated at a fluence of 235 J/cm2 was used in the biological assays. The behavior of mesenchymal stem cells from an umbilical cord vein was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a mineralization assay, and an alkaline phosphatase activity assay and by carrying out a quantitative real-time polymerase chain reaction for osteogenic markers. CHO-k1 cells were also exposed to titanium discs in the MTT assay. Results The best titanium surface was that produced by laser beam irradiation at 235 J/cm2 fluence. Cell proliferation analysis revealed that the CHO-k1 and mesenchymal stem cells behaved differently. The laser-processed titanium surface increased the proliferation of CHO-k1 cells, reduced the proliferation of mesenchymal stem cells, upregulated the expression of the osteogenic markers, and enhanced alkaline phosphatase activity. Conclusions The laser-treated titanium surface modulated cellular behavior depending on the cell type, and stimulated osteogenic differentiation. This evidence supports the potential use of laser-processed titanium surfaces as bone implant materials, and their use in regenerative medicine could promote better outcomes.http://link.springer.com/article/10.1186/s13287-017-0717-9TitaniumLaser beam (Yb-YAG)Surface modificationHuman umbilical cordMesenchymal stem cellsOsteoinduction |
| spellingShingle | Tatiana A. B. Bressel Jana Dara Freires de Queiroz Susana Margarida Gomes Moreira Jéssyca T. da Fonseca Edson A. Filho Antônio Carlos Guastaldi Silvia Regina Batistuzzo de Medeiros Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells Stem Cell Research & Therapy Titanium Laser beam (Yb-YAG) Surface modification Human umbilical cord Mesenchymal stem cells Osteoinduction |
| title | Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells |
| title_full | Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells |
| title_fullStr | Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells |
| title_full_unstemmed | Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells |
| title_short | Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells |
| title_sort | laser modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells |
| topic | Titanium Laser beam (Yb-YAG) Surface modification Human umbilical cord Mesenchymal stem cells Osteoinduction |
| url | http://link.springer.com/article/10.1186/s13287-017-0717-9 |
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