Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for d...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2003-01-01
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| Series: | Journal of the Brazilian Chemical Society |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000400002 |
| _version_ | 1818137652823588864 |
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| author | Skládal Petr |
| author_facet | Skládal Petr |
| author_sort | Skládal Petr |
| collection | DOAJ |
| description | This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers. |
| first_indexed | 2024-12-11T09:59:43Z |
| format | Article |
| id | doaj.art-c0bfe53a06ec46ed8499b26de5ba195f |
| institution | Directory Open Access Journal |
| issn | 0103-5053 |
| language | English |
| last_indexed | 2024-12-11T09:59:43Z |
| publishDate | 2003-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Journal of the Brazilian Chemical Society |
| spelling | doaj.art-c0bfe53a06ec46ed8499b26de5ba195f2022-12-22T01:12:10ZengSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society0103-50532003-01-01144491502Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactionsSkládal PetrThis review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000400002piezoelectric quartz crystal sensorpiezoelectric immunosensorsaffinity interactionskinetic parameters |
| spellingShingle | Skládal Petr Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions Journal of the Brazilian Chemical Society piezoelectric quartz crystal sensor piezoelectric immunosensors affinity interactions kinetic parameters |
| title | Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions |
| title_full | Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions |
| title_fullStr | Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions |
| title_full_unstemmed | Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions |
| title_short | Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions |
| title_sort | piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions |
| topic | piezoelectric quartz crystal sensor piezoelectric immunosensors affinity interactions kinetic parameters |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000400002 |
| work_keys_str_mv | AT skladalpetr piezoelectricquartzcrystalsensorsappliedforbioanalyticalassaysandcharacterizationofaffinityinteractions |