Multilocus sequence typing of commensal and enteropathogenic Escherichia coli from domestic and wild lagomorphs in Italy
The aim of the study was to determine the multilocus sequence types of <em>Escherichia coli</em> from diseased farm rabbits and apparently healthy wild lagomorphs, and the genetic relatedness among them. Fifty-five enteropathogenic <em>E. coli</em> from reared rabbits and 32...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2015-12-01
|
| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/4139 |
| _version_ | 1818973109636562944 |
|---|---|
| author | Giorgia Dotto Giovanni Franzo Guido Grilli Alessandra Piccirillo |
| author_facet | Giorgia Dotto Giovanni Franzo Guido Grilli Alessandra Piccirillo |
| author_sort | Giorgia Dotto |
| collection | DOAJ |
| description | The aim of the study was to determine the multilocus sequence types of <em>Escherichia coli</em> from diseased farm rabbits and apparently healthy wild lagomorphs, and the genetic relatedness among them. Fifty-five enteropathogenic <em>E. coli</em> from reared rabbits and 32 from wild rabbits and hares were characterised by multilocus sequence typing (MLST) according to the Michigan State University <em>Ec</em>MLST scheme. Isolates were differentiated into 37 sequence types (STs), which were grouped into 8 clonal complexes (CCs). The most common ST was ST140 (CC31), followed by ST238 and ST119 (CC17). MLST analysis revealed 22 novel STs. Phylogenetic analyses showed a heterogeneous distribution of STs into 3 clusters of genetically related strains. The genetic relationship among STs of different origin and the detection of new, as well as previously described STs as human pathogens, indicate a widespread distribution and adaptability of particular lineages to different hosts. These findings highlight the need for further research to improve the knowledge about <em>E. coli</em> populations colonising the gut of lagomorphs and their zoonotic potential. |
| first_indexed | 2024-12-20T15:18:56Z |
| format | Article |
| id | doaj.art-c0c02619141b4db2b794789504069781 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-12-20T15:18:56Z |
| publishDate | 2015-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-c0c02619141b4db2b7947895040697812022-12-21T19:36:07ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2015-12-0114410.4081/ijas.2015.41392431Multilocus sequence typing of commensal and enteropathogenic Escherichia coli from domestic and wild lagomorphs in ItalyGiorgia Dotto0Giovanni Franzo1Guido Grilli2Alessandra Piccirillo3Dipartimento di Biomedicina Comparata e Alimentazione, University of PaduaDipartimento di Medicina Animale, Produzioni e Salute, University of PaduaDipartimento di Scienze Veterinarie e Sanità Pubblica, University of MilanDipartimento di Biomedicina Comparata e Alimentazione, University of PaduaThe aim of the study was to determine the multilocus sequence types of <em>Escherichia coli</em> from diseased farm rabbits and apparently healthy wild lagomorphs, and the genetic relatedness among them. Fifty-five enteropathogenic <em>E. coli</em> from reared rabbits and 32 from wild rabbits and hares were characterised by multilocus sequence typing (MLST) according to the Michigan State University <em>Ec</em>MLST scheme. Isolates were differentiated into 37 sequence types (STs), which were grouped into 8 clonal complexes (CCs). The most common ST was ST140 (CC31), followed by ST238 and ST119 (CC17). MLST analysis revealed 22 novel STs. Phylogenetic analyses showed a heterogeneous distribution of STs into 3 clusters of genetically related strains. The genetic relationship among STs of different origin and the detection of new, as well as previously described STs as human pathogens, indicate a widespread distribution and adaptability of particular lineages to different hosts. These findings highlight the need for further research to improve the knowledge about <em>E. coli</em> populations colonising the gut of lagomorphs and their zoonotic potential.http://www.aspajournal.it/index.php/ijas/article/view/4139Farm rabbitsWild rabbitsHaresEscherichia coli |
| spellingShingle | Giorgia Dotto Giovanni Franzo Guido Grilli Alessandra Piccirillo Multilocus sequence typing of commensal and enteropathogenic Escherichia coli from domestic and wild lagomorphs in Italy Italian Journal of Animal Science Farm rabbits Wild rabbits Hares Escherichia coli |
| title | Multilocus sequence typing of commensal and enteropathogenic Escherichia coli from domestic and wild lagomorphs in Italy |
| title_full | Multilocus sequence typing of commensal and enteropathogenic Escherichia coli from domestic and wild lagomorphs in Italy |
| title_fullStr | Multilocus sequence typing of commensal and enteropathogenic Escherichia coli from domestic and wild lagomorphs in Italy |
| title_full_unstemmed | Multilocus sequence typing of commensal and enteropathogenic Escherichia coli from domestic and wild lagomorphs in Italy |
| title_short | Multilocus sequence typing of commensal and enteropathogenic Escherichia coli from domestic and wild lagomorphs in Italy |
| title_sort | multilocus sequence typing of commensal and enteropathogenic escherichia coli from domestic and wild lagomorphs in italy |
| topic | Farm rabbits Wild rabbits Hares Escherichia coli |
| url | http://www.aspajournal.it/index.php/ijas/article/view/4139 |
| work_keys_str_mv | AT giorgiadotto multilocussequencetypingofcommensalandenteropathogenicescherichiacolifromdomesticandwildlagomorphsinitaly AT giovannifranzo multilocussequencetypingofcommensalandenteropathogenicescherichiacolifromdomesticandwildlagomorphsinitaly AT guidogrilli multilocussequencetypingofcommensalandenteropathogenicescherichiacolifromdomesticandwildlagomorphsinitaly AT alessandrapiccirillo multilocussequencetypingofcommensalandenteropathogenicescherichiacolifromdomesticandwildlagomorphsinitaly |