EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY
The interactions between dietary zinc, copper and iron and their effects on antioxidant enzymes activity [superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST)] were examined. Fifty-four rats weighting approximately between 80-100 grams were divided into nine groups (E1- E9)...
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| Format: | Article |
| Language: | Arabic |
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The Union of Arab Universities
2007-09-01
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| Series: | Arab Universities Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://ajs.journals.ekb.eg/article_14927_fa27b893de4ff4a8531c8eb0f54d9afb.pdf |
| _version_ | 1797269691438202880 |
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| author | Abeer El-Dakak Mona Ahmed Dalia El-Nahal |
| author_facet | Abeer El-Dakak Mona Ahmed Dalia El-Nahal |
| author_sort | Abeer El-Dakak |
| collection | DOAJ |
| description | The interactions between dietary zinc, copper and iron and their effects on antioxidant enzymes activity [superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST)] were examined. Fifty-four rats weighting approximately between 80-100 grams were divided into nine groups (E1- E9). Each group containing six rats was fed on basal diet with varying amount of Zn (2.5, 35 or 175 mg Zn/kg diet), Cu (1.2, 6.3, or 25 mg Cu/kg diet) and Fe (10, 50 or 230 mg Fe/kg diet). The second group (E2) was fed a diet defi-cient in all studied elements (2.5mg Zn, 1.2mg Cu, and 10mg Fe per 1 kg diet). The results of E2 showed a decrease in SOD and CAT activity and increase GST in plasma erythrocytes and tissues. Moreover, plasma thiobarbituric acid reactive sub-stances (TBARs) and hydrogen peroxide produc-tion in E2 were found to be higher than those in other groups. The high activity of lactic acid de-hydrogenase (LDH) in the plasma indicated that cell membrane damage was occurred. This dam-age was accompanied by elevation of lipid peroxi-dation and in the same time by reduction of SOD and CAT activity. Supplementation of basal diet with the different concentrations of studied miner-als led to improve the activity of antioxidants en-zymes (SOD and CAT) and decrease in TBARs and LDH activity. |
| first_indexed | 2024-04-25T01:52:24Z |
| format | Article |
| id | doaj.art-c0c317b492c043b5a17779b8659f04e8 |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:52:24Z |
| publishDate | 2007-09-01 |
| publisher | The Union of Arab Universities |
| record_format | Article |
| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-c0c317b492c043b5a17779b8659f04e82024-03-07T17:47:33ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852007-09-0115237138210.21608/ajs.2007.1492714927EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITYAbeer El-Dakak0Mona Ahmed1Dalia El-Nahal2Special Food and Nutrition Dept., Food Technology Research Institute, Agric. Res. Center, Giza, EgyptSpecial Food and Nutrition Dept., Food Technology Research Institute, Agric. Res. Center, Giza, EgyptSpecial Food and Nutrition Dept., Food Technology Research Institute, Agric. Res. Center, Giza, EgyptThe interactions between dietary zinc, copper and iron and their effects on antioxidant enzymes activity [superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST)] were examined. Fifty-four rats weighting approximately between 80-100 grams were divided into nine groups (E1- E9). Each group containing six rats was fed on basal diet with varying amount of Zn (2.5, 35 or 175 mg Zn/kg diet), Cu (1.2, 6.3, or 25 mg Cu/kg diet) and Fe (10, 50 or 230 mg Fe/kg diet). The second group (E2) was fed a diet defi-cient in all studied elements (2.5mg Zn, 1.2mg Cu, and 10mg Fe per 1 kg diet). The results of E2 showed a decrease in SOD and CAT activity and increase GST in plasma erythrocytes and tissues. Moreover, plasma thiobarbituric acid reactive sub-stances (TBARs) and hydrogen peroxide produc-tion in E2 were found to be higher than those in other groups. The high activity of lactic acid de-hydrogenase (LDH) in the plasma indicated that cell membrane damage was occurred. This dam-age was accompanied by elevation of lipid peroxi-dation and in the same time by reduction of SOD and CAT activity. Supplementation of basal diet with the different concentrations of studied miner-als led to improve the activity of antioxidants en-zymes (SOD and CAT) and decrease in TBARs and LDH activity.https://ajs.journals.ekb.eg/article_14927_fa27b893de4ff4a8531c8eb0f54d9afb.pdfzinccopperironsuperoxide dis-mutasecatalaseglutathione-s-transferase |
| spellingShingle | Abeer El-Dakak Mona Ahmed Dalia El-Nahal EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY Arab Universities Journal of Agricultural Sciences zinc copper iron superoxide dis-mutase catalase glutathione-s-transferase |
| title | EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY |
| title_full | EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY |
| title_fullStr | EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY |
| title_full_unstemmed | EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY |
| title_short | EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY |
| title_sort | effect of dietary zinc copper and iron levels on superoxide dismutase catalase and glutathione s transferase activity |
| topic | zinc copper iron superoxide dis-mutase catalase glutathione-s-transferase |
| url | https://ajs.journals.ekb.eg/article_14927_fa27b893de4ff4a8531c8eb0f54d9afb.pdf |
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