Characterization of phyto-components with antimicrobial traits in supercritical carbon dioxide and soxhlet Prosopis juliflora leaves extract using GC-MS
Abstract This study aimed to screen the bioactive compounds from Prosopis juliflora leaf supercritical fluid extract and to assess its antimicrobial properties. Supercritical carbon dioxide and Soxhlet methods were used for extraction. The extract was subjected to Gas Chromatography-Mass Spectromete...
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| Format: | Article |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-30390-9 |
| _version_ | 1827984230892699648 |
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| author | Nagaraj M. Naik M. Krishnaveni M. Mahadevswamy M. Bheemanna Udaykumar Nidoni Vasant Kumar K. Tejashri |
| author_facet | Nagaraj M. Naik M. Krishnaveni M. Mahadevswamy M. Bheemanna Udaykumar Nidoni Vasant Kumar K. Tejashri |
| author_sort | Nagaraj M. Naik |
| collection | DOAJ |
| description | Abstract This study aimed to screen the bioactive compounds from Prosopis juliflora leaf supercritical fluid extract and to assess its antimicrobial properties. Supercritical carbon dioxide and Soxhlet methods were used for extraction. The extract was subjected to Gas Chromatography-Mass Spectrometer (GC-MS) and Fourier Transform Infrared for the characterization of the phyto-components. When compared to soxhlet extraction, more components (35) were eluted by supercritical fluid extraction (SFE), according to GC-MS screening. Rhizoctonia bataticola, Alternaria alternata, and Colletotrichum gloeosporioides were all successfully inhibited by P. juliflora leaf SFE extract, which demonstrated strong antifungal properties with mycelium percent inhibition of 94.07%, 93.15%, and 92.43%, respectively, compared to extract from Soxhlet, which registered 55.31%, 75.63% and 45.13% mycelium inhibition respectively. Also, SFE P. juliflora extracts registered higher zone of inhibition 13.90 mm, 14.47 mm and 14.53 mm against all three test food-borne bacterial pathogens viz Escherichia coli, Salmonella enterica and Staphylococcus aureus respectively. Results obtained from GC-MS screening revealed that SFE is more efficient than soxhlet extraction in recovering the phyto-components. P. juliflora may provide antimicrobial agents, a novel natural inhibitory metabolite. |
| first_indexed | 2024-04-09T22:57:39Z |
| format | Article |
| id | doaj.art-c0c6af5b5d8349b2860d17f50c897fb1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:57:39Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c0c6af5b5d8349b2860d17f50c897fb12023-03-22T11:12:17ZengNature PortfolioScientific Reports2045-23222023-03-011311910.1038/s41598-023-30390-9Characterization of phyto-components with antimicrobial traits in supercritical carbon dioxide and soxhlet Prosopis juliflora leaves extract using GC-MSNagaraj M. Naik0M. Krishnaveni1M. Mahadevswamy2M. Bheemanna3Udaykumar Nidoni4Vasant Kumar5K. Tejashri6Pesticide Residue and Food Quality Analysis Laboratory, University of Agricultural SciencesDepartment of Agricultural Microbiology, University of Agricultural SciencesDepartment of Agricultural Microbiology, University of Agricultural SciencesPesticide Residue and Food Quality Analysis Laboratory, University of Agricultural SciencesDepartment of Processing and Food Engineering, University of Agricultural SciencesPesticide Residue and Food Quality Analysis Laboratory, University of Agricultural SciencesPesticide Residue and Food Quality Analysis Laboratory, University of Agricultural SciencesAbstract This study aimed to screen the bioactive compounds from Prosopis juliflora leaf supercritical fluid extract and to assess its antimicrobial properties. Supercritical carbon dioxide and Soxhlet methods were used for extraction. The extract was subjected to Gas Chromatography-Mass Spectrometer (GC-MS) and Fourier Transform Infrared for the characterization of the phyto-components. When compared to soxhlet extraction, more components (35) were eluted by supercritical fluid extraction (SFE), according to GC-MS screening. Rhizoctonia bataticola, Alternaria alternata, and Colletotrichum gloeosporioides were all successfully inhibited by P. juliflora leaf SFE extract, which demonstrated strong antifungal properties with mycelium percent inhibition of 94.07%, 93.15%, and 92.43%, respectively, compared to extract from Soxhlet, which registered 55.31%, 75.63% and 45.13% mycelium inhibition respectively. Also, SFE P. juliflora extracts registered higher zone of inhibition 13.90 mm, 14.47 mm and 14.53 mm against all three test food-borne bacterial pathogens viz Escherichia coli, Salmonella enterica and Staphylococcus aureus respectively. Results obtained from GC-MS screening revealed that SFE is more efficient than soxhlet extraction in recovering the phyto-components. P. juliflora may provide antimicrobial agents, a novel natural inhibitory metabolite.https://doi.org/10.1038/s41598-023-30390-9 |
| spellingShingle | Nagaraj M. Naik M. Krishnaveni M. Mahadevswamy M. Bheemanna Udaykumar Nidoni Vasant Kumar K. Tejashri Characterization of phyto-components with antimicrobial traits in supercritical carbon dioxide and soxhlet Prosopis juliflora leaves extract using GC-MS Scientific Reports |
| title | Characterization of phyto-components with antimicrobial traits in supercritical carbon dioxide and soxhlet Prosopis juliflora leaves extract using GC-MS |
| title_full | Characterization of phyto-components with antimicrobial traits in supercritical carbon dioxide and soxhlet Prosopis juliflora leaves extract using GC-MS |
| title_fullStr | Characterization of phyto-components with antimicrobial traits in supercritical carbon dioxide and soxhlet Prosopis juliflora leaves extract using GC-MS |
| title_full_unstemmed | Characterization of phyto-components with antimicrobial traits in supercritical carbon dioxide and soxhlet Prosopis juliflora leaves extract using GC-MS |
| title_short | Characterization of phyto-components with antimicrobial traits in supercritical carbon dioxide and soxhlet Prosopis juliflora leaves extract using GC-MS |
| title_sort | characterization of phyto components with antimicrobial traits in supercritical carbon dioxide and soxhlet prosopis juliflora leaves extract using gc ms |
| url | https://doi.org/10.1038/s41598-023-30390-9 |
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