Administration of silver nanoparticles affects ovarian steroidogenesis and may influence thyroid hormone metabolism in hens (Gallus domesticus)
This study aimed to determine the in vivo effect of silver nanoparticles (AgNPs) on the concentration of sex steroids (progesterone – P4, estradiol – E2, testosterone – T) and thyroid hormones (thyroxine – T4, triiodothyronine – T3) in the blood plasma as well as the messenger ribonucleic acid (mRNA...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651320312641 |
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| author | Dorota Katarzyńska-Banasik Małgorzata Grzesiak Kinga Kowalik Andrzej Sechman |
| author_facet | Dorota Katarzyńska-Banasik Małgorzata Grzesiak Kinga Kowalik Andrzej Sechman |
| author_sort | Dorota Katarzyńska-Banasik |
| collection | DOAJ |
| description | This study aimed to determine the in vivo effect of silver nanoparticles (AgNPs) on the concentration of sex steroids (progesterone – P4, estradiol – E2, testosterone – T) and thyroid hormones (thyroxine – T4, triiodothyronine – T3) in the blood plasma as well as the messenger ribonucleic acid (mRNA) and protein expression of HSD3β, CYP17A1 and CYP19A1 enzymes and steroid hormone concentrations in chicken ovarian follicles. AgNPs did not affect serum steroid hormone levels, but increased T3 levels depending on the size and concentration of AgNPs. At the level of ovarian tissues, AgNPs: (i) affected the levels of E2 and T in prehierachical follicles; (ii) reduced the expression of CYP19A1 mRNA and protein and consequently diminished E2 concentration in small white follicles; and (iii) increased the expression of CYP17A1 mRNA in large white follicles, without changing its protein expression. The results indicate that AgNPs affect chicken ovarian steroidogenesis. The effects of AgNPs depend on exposure time, the type of follicle and the degree of its development and are associated with the modulation of steroidogenic gene expression and E2 and T synthesis. Prehierachical follicles seem to be more susceptible to AgNPs than preovulatory ones. In conclusion, AgNPs by targeting the chicken ovary may indirectly influence the selection processes of prehierarchical follicles to the pre-ovulatory hierarchy and disturb the ovarian steroidogenesis. Furthermore, AgNPs may affect thyroid hormone metabolism in different ways by size which in turn may influence energy homeostasis of the target cells. |
| first_indexed | 2024-12-17T04:53:05Z |
| format | Article |
| id | doaj.art-c0c80cbb487d46688412959d0699a929 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-17T04:53:05Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-c0c80cbb487d46688412959d0699a9292022-12-21T22:02:50ZengElsevierEcotoxicology and Environmental Safety0147-65132021-01-01208111427Administration of silver nanoparticles affects ovarian steroidogenesis and may influence thyroid hormone metabolism in hens (Gallus domesticus)Dorota Katarzyńska-Banasik0Małgorzata Grzesiak1Kinga Kowalik2Andrzej Sechman3Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059 Krakow, Poland; Corresponding author.Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, PolandDepartment of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059 Krakow, PolandDepartment of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059 Krakow, PolandThis study aimed to determine the in vivo effect of silver nanoparticles (AgNPs) on the concentration of sex steroids (progesterone – P4, estradiol – E2, testosterone – T) and thyroid hormones (thyroxine – T4, triiodothyronine – T3) in the blood plasma as well as the messenger ribonucleic acid (mRNA) and protein expression of HSD3β, CYP17A1 and CYP19A1 enzymes and steroid hormone concentrations in chicken ovarian follicles. AgNPs did not affect serum steroid hormone levels, but increased T3 levels depending on the size and concentration of AgNPs. At the level of ovarian tissues, AgNPs: (i) affected the levels of E2 and T in prehierachical follicles; (ii) reduced the expression of CYP19A1 mRNA and protein and consequently diminished E2 concentration in small white follicles; and (iii) increased the expression of CYP17A1 mRNA in large white follicles, without changing its protein expression. The results indicate that AgNPs affect chicken ovarian steroidogenesis. The effects of AgNPs depend on exposure time, the type of follicle and the degree of its development and are associated with the modulation of steroidogenic gene expression and E2 and T synthesis. Prehierachical follicles seem to be more susceptible to AgNPs than preovulatory ones. In conclusion, AgNPs by targeting the chicken ovary may indirectly influence the selection processes of prehierarchical follicles to the pre-ovulatory hierarchy and disturb the ovarian steroidogenesis. Furthermore, AgNPs may affect thyroid hormone metabolism in different ways by size which in turn may influence energy homeostasis of the target cells.http://www.sciencedirect.com/science/article/pii/S0147651320312641Silver nanoparticlesSteroidogenesisThyroid hormonesSex steroidsChicken |
| spellingShingle | Dorota Katarzyńska-Banasik Małgorzata Grzesiak Kinga Kowalik Andrzej Sechman Administration of silver nanoparticles affects ovarian steroidogenesis and may influence thyroid hormone metabolism in hens (Gallus domesticus) Ecotoxicology and Environmental Safety Silver nanoparticles Steroidogenesis Thyroid hormones Sex steroids Chicken |
| title | Administration of silver nanoparticles affects ovarian steroidogenesis and may influence thyroid hormone metabolism in hens (Gallus domesticus) |
| title_full | Administration of silver nanoparticles affects ovarian steroidogenesis and may influence thyroid hormone metabolism in hens (Gallus domesticus) |
| title_fullStr | Administration of silver nanoparticles affects ovarian steroidogenesis and may influence thyroid hormone metabolism in hens (Gallus domesticus) |
| title_full_unstemmed | Administration of silver nanoparticles affects ovarian steroidogenesis and may influence thyroid hormone metabolism in hens (Gallus domesticus) |
| title_short | Administration of silver nanoparticles affects ovarian steroidogenesis and may influence thyroid hormone metabolism in hens (Gallus domesticus) |
| title_sort | administration of silver nanoparticles affects ovarian steroidogenesis and may influence thyroid hormone metabolism in hens gallus domesticus |
| topic | Silver nanoparticles Steroidogenesis Thyroid hormones Sex steroids Chicken |
| url | http://www.sciencedirect.com/science/article/pii/S0147651320312641 |
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