Modelling the development of biological structures displaying longitudinal geometries in vitro: culturing pluripotent stem cells on plasma-treated, growth factor-coupled polycaprolactone fibres
Many biological structures such as nerves, blood and lymphatic vessels, and muscle fibres exhibit longitudinal geometries with distinct cell types extending along both the length and width of internal linear axes. Modelling these three-dimensional structures in vitro is challenging: the best-defined...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-03-01
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| Series: | Engineered Regeneration |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666138123000646 |
| _version_ | 1827356749189873664 |
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| author | Badwi B. Boumelhem Stuart T. Fraser Syamak Farajikhah Rachel A. Shparberg Michael B. Morris Marcela M.M. Bilek Anyu Zhang Behnam Akhavan Simon Fleming Maryanne Large |
| author_facet | Badwi B. Boumelhem Stuart T. Fraser Syamak Farajikhah Rachel A. Shparberg Michael B. Morris Marcela M.M. Bilek Anyu Zhang Behnam Akhavan Simon Fleming Maryanne Large |
| author_sort | Badwi B. Boumelhem |
| collection | DOAJ |
| description | Many biological structures such as nerves, blood and lymphatic vessels, and muscle fibres exhibit longitudinal geometries with distinct cell types extending along both the length and width of internal linear axes. Modelling these three-dimensional structures in vitro is challenging: the best-defined stem-cell differentiation systems are monolayer cultures or organoids using pluripotent stem cells. Pluripotent stem cells can differentiate into functionally mature cells depending on the signals received, holding great promise for regenerative medicine. However, the integration of in vitro differentiated cell types into diseased tissue remains a challenge. Engineered scaffolds can bridge this gap if the appropriate signalling systems are incorporated into the scaffold. Here, we have taken a biomimicry approach to generate longitudinal structures in vitro. In this approach, mouse embryonic stem cells are directed to differentiate to specific cell types on the surface of polycaprolactone (PCL) fibres treated by plasma-immersion ion implantation and to which with lineage-specifying molecules have been covalently immobilised. We demonstrate the simplicity and utility of our method for efficiently generating high yields of the following cell types from these pluripotent stem cells: neurons, vascular endothelial cells, osteoclasts, adipocytes, and cells of the erythroid, myeloid, and lymphoid lineages. Strategically arranged plasma-treated scaffolds with differentiated cell types could ultimately serve as a means for the repair or treatment of diseased or damaged tissue. |
| first_indexed | 2024-03-08T05:14:27Z |
| format | Article |
| id | doaj.art-c0cabfb334234ad2a64bbc76e3b64b4d |
| institution | Directory Open Access Journal |
| issn | 2666-1381 |
| language | English |
| last_indexed | 2024-03-08T05:14:27Z |
| publishDate | 2024-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Engineered Regeneration |
| spelling | doaj.art-c0cabfb334234ad2a64bbc76e3b64b4d2024-02-07T04:45:50ZengKeAi Communications Co., Ltd.Engineered Regeneration2666-13812024-03-0151124138Modelling the development of biological structures displaying longitudinal geometries in vitro: culturing pluripotent stem cells on plasma-treated, growth factor-coupled polycaprolactone fibresBadwi B. Boumelhem0Stuart T. Fraser1Syamak Farajikhah2Rachel A. Shparberg3Michael B. Morris4Marcela M.M. Bilek5Anyu Zhang6Behnam Akhavan7Simon Fleming8Maryanne Large9School of Physics, Faculty of Science, University of Sydney, Australia; Sydney Nano Institute, University of Sydney, Australia; School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Australia; School of Biomedical Engineering, Faculty of Engineering, University of Sydney, AustraliaSydney Nano Institute, University of Sydney, Australia; School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Australia; School of Biomedical Engineering, Faculty of Engineering, University of Sydney, AustraliaSchool of Physics, Faculty of Science, University of Sydney, Australia; Sydney Nano Institute, University of Sydney, Australia; School of Chemical and Biomolecular Engineering, University of Sydney, Australia; School of Biomedical Engineering, Faculty of Engineering, University of Sydney, AustraliaSchool of Physics, Faculty of Science, University of Sydney, Australia; School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, AustraliaSchool of Medical Sciences, Faculty of Medicine and Health, University of Sydney, AustraliaSydney Nano Institute, University of Sydney, Australia; School of Biomedical Engineering, Faculty of Engineering, University of Sydney, AustraliaSchool of Biomedical Engineering, Faculty of Engineering, University of Sydney, AustraliaSchool of Biomedical Engineering, Faculty of Engineering, University of Sydney, Australia; School of Engineering, University of Newcastle, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute (HMRI), Precision Medicine Program, New Lambton Heights NSW 2305, AustraliaSchool of Physics, Faculty of Science, University of Sydney, Australia; Sydney Nano Institute, University of Sydney, AustraliaSchool of Physics, Faculty of Science, University of Sydney, Australia; Sydney Nano Institute, University of Sydney, Australia; Corresponding author at: School of Physics, Building A28, Faculty of Science, University of Sydney, Camperdown, NSW 2006 Australia.Many biological structures such as nerves, blood and lymphatic vessels, and muscle fibres exhibit longitudinal geometries with distinct cell types extending along both the length and width of internal linear axes. Modelling these three-dimensional structures in vitro is challenging: the best-defined stem-cell differentiation systems are monolayer cultures or organoids using pluripotent stem cells. Pluripotent stem cells can differentiate into functionally mature cells depending on the signals received, holding great promise for regenerative medicine. However, the integration of in vitro differentiated cell types into diseased tissue remains a challenge. Engineered scaffolds can bridge this gap if the appropriate signalling systems are incorporated into the scaffold. Here, we have taken a biomimicry approach to generate longitudinal structures in vitro. In this approach, mouse embryonic stem cells are directed to differentiate to specific cell types on the surface of polycaprolactone (PCL) fibres treated by plasma-immersion ion implantation and to which with lineage-specifying molecules have been covalently immobilised. We demonstrate the simplicity and utility of our method for efficiently generating high yields of the following cell types from these pluripotent stem cells: neurons, vascular endothelial cells, osteoclasts, adipocytes, and cells of the erythroid, myeloid, and lymphoid lineages. Strategically arranged plasma-treated scaffolds with differentiated cell types could ultimately serve as a means for the repair or treatment of diseased or damaged tissue.http://www.sciencedirect.com/science/article/pii/S2666138123000646Pluripotent stem cellsLineage commitmentGrowth factorsBiomaterial engineeringPlasma-immersion ion implantationCovalent biomolecule attachment |
| spellingShingle | Badwi B. Boumelhem Stuart T. Fraser Syamak Farajikhah Rachel A. Shparberg Michael B. Morris Marcela M.M. Bilek Anyu Zhang Behnam Akhavan Simon Fleming Maryanne Large Modelling the development of biological structures displaying longitudinal geometries in vitro: culturing pluripotent stem cells on plasma-treated, growth factor-coupled polycaprolactone fibres Engineered Regeneration Pluripotent stem cells Lineage commitment Growth factors Biomaterial engineering Plasma-immersion ion implantation Covalent biomolecule attachment |
| title | Modelling the development of biological structures displaying longitudinal geometries in vitro: culturing pluripotent stem cells on plasma-treated, growth factor-coupled polycaprolactone fibres |
| title_full | Modelling the development of biological structures displaying longitudinal geometries in vitro: culturing pluripotent stem cells on plasma-treated, growth factor-coupled polycaprolactone fibres |
| title_fullStr | Modelling the development of biological structures displaying longitudinal geometries in vitro: culturing pluripotent stem cells on plasma-treated, growth factor-coupled polycaprolactone fibres |
| title_full_unstemmed | Modelling the development of biological structures displaying longitudinal geometries in vitro: culturing pluripotent stem cells on plasma-treated, growth factor-coupled polycaprolactone fibres |
| title_short | Modelling the development of biological structures displaying longitudinal geometries in vitro: culturing pluripotent stem cells on plasma-treated, growth factor-coupled polycaprolactone fibres |
| title_sort | modelling the development of biological structures displaying longitudinal geometries in vitro culturing pluripotent stem cells on plasma treated growth factor coupled polycaprolactone fibres |
| topic | Pluripotent stem cells Lineage commitment Growth factors Biomaterial engineering Plasma-immersion ion implantation Covalent biomolecule attachment |
| url | http://www.sciencedirect.com/science/article/pii/S2666138123000646 |
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