An approach for reliably investigating hippocampal sharp wave-ripples in vitro.
<h4>Background</h4>Among the various hippocampal network patterns, sharp wave-ripples (SPW-R) are currently the mechanistically least understood. Although accurate information on synaptic interactions between the participating neurons is essential for comprehensive understanding of the n...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2009-09-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19738897/?tool=EBI |
| _version_ | 1818983955440861184 |
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| author | Nikolaus Maier Genela Morris Friedrich W Johenning Dietmar Schmitz |
| author_facet | Nikolaus Maier Genela Morris Friedrich W Johenning Dietmar Schmitz |
| author_sort | Nikolaus Maier |
| collection | DOAJ |
| description | <h4>Background</h4>Among the various hippocampal network patterns, sharp wave-ripples (SPW-R) are currently the mechanistically least understood. Although accurate information on synaptic interactions between the participating neurons is essential for comprehensive understanding of the network function during complex activities like SPW-R, such knowledge is currently notably scarce.<h4>Methodology/principal findings</h4>We demonstrate an in vitro approach to SPW-R that offers a simple experimental tool allowing detailed analysis of mechanisms governing the sharp wave-state of the hippocampus. We combine interface storage of slices with modifications of a conventional submerged recording system and established in vitro SPW-R comparable to their in vivo counterpart. We show that slice storage in the interface chamber close to physiological temperature is the required condition to preserve network integrity that is necessary for the generation of SPW-R. Moreover, we demonstrate the utility of our method for studying synaptic and network properties of SPW-R, using electrophysiological and imaging methods that can only be applied in the submerged system.<h4>Conclusions/significance</h4>The approach presented here demonstrates a reliable and experimentally simple strategy for studying hippocampal sharp wave-ripples. Given its utility and easy application we expect our model to foster the generation of new insight into the network physiology underlying SPW-R. |
| first_indexed | 2024-12-20T18:11:20Z |
| format | Article |
| id | doaj.art-c0cbcb4adffe493c952c24c159e6dab3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T18:11:20Z |
| publishDate | 2009-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c0cbcb4adffe493c952c24c159e6dab32022-12-21T19:30:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-09-0149e692510.1371/journal.pone.0006925An approach for reliably investigating hippocampal sharp wave-ripples in vitro.Nikolaus MaierGenela MorrisFriedrich W JohenningDietmar Schmitz<h4>Background</h4>Among the various hippocampal network patterns, sharp wave-ripples (SPW-R) are currently the mechanistically least understood. Although accurate information on synaptic interactions between the participating neurons is essential for comprehensive understanding of the network function during complex activities like SPW-R, such knowledge is currently notably scarce.<h4>Methodology/principal findings</h4>We demonstrate an in vitro approach to SPW-R that offers a simple experimental tool allowing detailed analysis of mechanisms governing the sharp wave-state of the hippocampus. We combine interface storage of slices with modifications of a conventional submerged recording system and established in vitro SPW-R comparable to their in vivo counterpart. We show that slice storage in the interface chamber close to physiological temperature is the required condition to preserve network integrity that is necessary for the generation of SPW-R. Moreover, we demonstrate the utility of our method for studying synaptic and network properties of SPW-R, using electrophysiological and imaging methods that can only be applied in the submerged system.<h4>Conclusions/significance</h4>The approach presented here demonstrates a reliable and experimentally simple strategy for studying hippocampal sharp wave-ripples. Given its utility and easy application we expect our model to foster the generation of new insight into the network physiology underlying SPW-R.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19738897/?tool=EBI |
| spellingShingle | Nikolaus Maier Genela Morris Friedrich W Johenning Dietmar Schmitz An approach for reliably investigating hippocampal sharp wave-ripples in vitro. PLoS ONE |
| title | An approach for reliably investigating hippocampal sharp wave-ripples in vitro. |
| title_full | An approach for reliably investigating hippocampal sharp wave-ripples in vitro. |
| title_fullStr | An approach for reliably investigating hippocampal sharp wave-ripples in vitro. |
| title_full_unstemmed | An approach for reliably investigating hippocampal sharp wave-ripples in vitro. |
| title_short | An approach for reliably investigating hippocampal sharp wave-ripples in vitro. |
| title_sort | approach for reliably investigating hippocampal sharp wave ripples in vitro |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19738897/?tool=EBI |
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