MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma
MicroRNAs can regulate the function of ion channels in many organs. Based on our previous study we propose that miR-142a-39, which is highly expressed in denervated skeletal muscle, might affect cell excitability through similar mechanisms. In this study, we overexpressed or knocked down miR-142a-3p...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=7;spage=1617;epage=1622;aulast=Gu |
| _version_ | 1831696870582255616 |
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| author | Xin-Yi Gu Bo Jin Zhi-Dan Qi Xiao-Feng Yin |
| author_facet | Xin-Yi Gu Bo Jin Zhi-Dan Qi Xiao-Feng Yin |
| author_sort | Xin-Yi Gu |
| collection | DOAJ |
| description | MicroRNAs can regulate the function of ion channels in many organs. Based on our previous study we propose that miR-142a-39, which is highly expressed in denervated skeletal muscle, might affect cell excitability through similar mechanisms. In this study, we overexpressed or knocked down miR-142a-3p in C2C12 cells using a lentivirus method. After 7 days of differentiation culture, whole-cell currents were recorded. The results showed that overexpression of miR-142a-3p reduced the cell membrane capacitance, increased potassium current density and decreased calcium current density. Knockdown of miR-142a-3p reduced sodium ion channel current density. The results showed that change in miR-142a-3p expression affected the ion channel currents in C2C12 cells, suggesting its possible roles in muscle cell electrophysiology. This study was approved by the Animal Ethics Committee of Peking University in July 2020 (approval No. LA2017128). |
| first_indexed | 2024-12-20T13:21:13Z |
| format | Article |
| id | doaj.art-c0ce440f989f4a31b83301dbffed6464 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-20T13:21:13Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-c0ce440f989f4a31b83301dbffed64642022-12-21T19:39:24ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742022-01-011771617162210.4103/1673-5374.330620MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after traumaXin-Yi GuBo JinZhi-Dan QiXiao-Feng YinMicroRNAs can regulate the function of ion channels in many organs. Based on our previous study we propose that miR-142a-39, which is highly expressed in denervated skeletal muscle, might affect cell excitability through similar mechanisms. In this study, we overexpressed or knocked down miR-142a-3p in C2C12 cells using a lentivirus method. After 7 days of differentiation culture, whole-cell currents were recorded. The results showed that overexpression of miR-142a-3p reduced the cell membrane capacitance, increased potassium current density and decreased calcium current density. Knockdown of miR-142a-3p reduced sodium ion channel current density. The results showed that change in miR-142a-3p expression affected the ion channel currents in C2C12 cells, suggesting its possible roles in muscle cell electrophysiology. This study was approved by the Animal Ethics Committee of Peking University in July 2020 (approval No. LA2017128).http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=7;spage=1617;epage=1622;aulast=Guc2c12; denervation; ion channels; microrna; mir-142a-3p; muscle; patch clamp; potassium; sodium; whole-cell currents |
| spellingShingle | Xin-Yi Gu Bo Jin Zhi-Dan Qi Xiao-Feng Yin MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma Neural Regeneration Research c2c12; denervation; ion channels; microrna; mir-142a-3p; muscle; patch clamp; potassium; sodium; whole-cell currents |
| title | MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma |
| title_full | MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma |
| title_fullStr | MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma |
| title_full_unstemmed | MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma |
| title_short | MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma |
| title_sort | microrna is a potential target for therapies to improve the physiological function of skeletal muscle after trauma |
| topic | c2c12; denervation; ion channels; microrna; mir-142a-3p; muscle; patch clamp; potassium; sodium; whole-cell currents |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=7;spage=1617;epage=1622;aulast=Gu |
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