The In Vitro Protective Role of Bovine Lactoferrin on Intestinal Epithelial Barrier
The intestinal epithelial barrier plays a key protective role in the gut lumen. Bovine lactoferrin (bLF) has been reported to improve the intestinal epithelial barrier function, but its impact on tight junction (TJ) proteins has been rarely described. Human intestinal epithelial crypt cells (HIECs)...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/24/1/148 |
| _version_ | 1819116675245539328 |
|---|---|
| author | Xiao Zhao Xiao-Xi Xu Yang Liu En-Ze Xi Jing-Jing An Dina Tabys Ning Liu |
| author_facet | Xiao Zhao Xiao-Xi Xu Yang Liu En-Ze Xi Jing-Jing An Dina Tabys Ning Liu |
| author_sort | Xiao Zhao |
| collection | DOAJ |
| description | The intestinal epithelial barrier plays a key protective role in the gut lumen. Bovine lactoferrin (bLF) has been reported to improve the intestinal epithelial barrier function, but its impact on tight junction (TJ) proteins has been rarely described. Human intestinal epithelial crypt cells (HIECs) were more similar to those in the human small intestine, compared with the well-established Caco-2 cells. Accordingly, both HIECs and Caco-2 cells were investigated in this study to determine the effects of bioactive protein bLF on their growth promotion and intestinal barrier function. The results showed that bLF promoted cell growth and arrested cell-cycle progression at the G2/M-phase. Moreover, bLF decreased paracellular permeability and increased alkaline phosphatase activity and transepithelial electrical resistance, strengthening barrier function. Immunofluorescence, western blot and quantitative real-time polymerase chain reaction revealed that bLF significantly increased the expression of three tight junction proteins—claudin-1, occludin, and ZO-1—at both the mRNA and protein levels, and consequently strengthened the barrier function of the two cell models. bLF in general showed higher activity in Caco-2 cells, however, HIECs also exhibited desired responses to barrier function. Therefore, bLF may be incorporated into functional foods for treatment of inflammatory bowel diseases which are caused by loss of barrier integrity. |
| first_indexed | 2024-12-22T05:20:51Z |
| format | Article |
| id | doaj.art-c0cfb60a672447a1ac593cafe643e71a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T05:20:51Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c0cfb60a672447a1ac593cafe643e71a2022-12-21T18:37:44ZengMDPI AGMolecules1420-30492019-01-0124114810.3390/molecules24010148molecules24010148The In Vitro Protective Role of Bovine Lactoferrin on Intestinal Epithelial BarrierXiao Zhao0Xiao-Xi Xu1Yang Liu2En-Ze Xi3Jing-Jing An4Dina Tabys5Ning Liu6Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaChina Feihe Co. Ltd., Beijing 100015, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaThe intestinal epithelial barrier plays a key protective role in the gut lumen. Bovine lactoferrin (bLF) has been reported to improve the intestinal epithelial barrier function, but its impact on tight junction (TJ) proteins has been rarely described. Human intestinal epithelial crypt cells (HIECs) were more similar to those in the human small intestine, compared with the well-established Caco-2 cells. Accordingly, both HIECs and Caco-2 cells were investigated in this study to determine the effects of bioactive protein bLF on their growth promotion and intestinal barrier function. The results showed that bLF promoted cell growth and arrested cell-cycle progression at the G2/M-phase. Moreover, bLF decreased paracellular permeability and increased alkaline phosphatase activity and transepithelial electrical resistance, strengthening barrier function. Immunofluorescence, western blot and quantitative real-time polymerase chain reaction revealed that bLF significantly increased the expression of three tight junction proteins—claudin-1, occludin, and ZO-1—at both the mRNA and protein levels, and consequently strengthened the barrier function of the two cell models. bLF in general showed higher activity in Caco-2 cells, however, HIECs also exhibited desired responses to barrier function. Therefore, bLF may be incorporated into functional foods for treatment of inflammatory bowel diseases which are caused by loss of barrier integrity.http://www.mdpi.com/1420-3049/24/1/148bovine lactoferrintight junction proteinintestinal epithelial barrierhuman intestinal epithelial crypt cellspermeability |
| spellingShingle | Xiao Zhao Xiao-Xi Xu Yang Liu En-Ze Xi Jing-Jing An Dina Tabys Ning Liu The In Vitro Protective Role of Bovine Lactoferrin on Intestinal Epithelial Barrier Molecules bovine lactoferrin tight junction protein intestinal epithelial barrier human intestinal epithelial crypt cells permeability |
| title | The In Vitro Protective Role of Bovine Lactoferrin on Intestinal Epithelial Barrier |
| title_full | The In Vitro Protective Role of Bovine Lactoferrin on Intestinal Epithelial Barrier |
| title_fullStr | The In Vitro Protective Role of Bovine Lactoferrin on Intestinal Epithelial Barrier |
| title_full_unstemmed | The In Vitro Protective Role of Bovine Lactoferrin on Intestinal Epithelial Barrier |
| title_short | The In Vitro Protective Role of Bovine Lactoferrin on Intestinal Epithelial Barrier |
| title_sort | in vitro protective role of bovine lactoferrin on intestinal epithelial barrier |
| topic | bovine lactoferrin tight junction protein intestinal epithelial barrier human intestinal epithelial crypt cells permeability |
| url | http://www.mdpi.com/1420-3049/24/1/148 |
| work_keys_str_mv | AT xiaozhao theinvitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT xiaoxixu theinvitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT yangliu theinvitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT enzexi theinvitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT jingjingan theinvitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT dinatabys theinvitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT ningliu theinvitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT xiaozhao invitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT xiaoxixu invitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT yangliu invitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT enzexi invitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT jingjingan invitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT dinatabys invitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier AT ningliu invitroprotectiveroleofbovinelactoferrinonintestinalepithelialbarrier |