A Group I WRKY Gene, <i>TaWRKY133</i>, Negatively Regulates Drought Resistance in Transgenic Plants
WRKYs are one of the largest transcription factor (TF) families and play an important role in plant resistance to various stresses. TaWRKY133, a group I WRKY protein, responds to a variety of abiotic stresses, including PEG treatment. The TaWRKY133 protein is located in the nucleus of tobacco epider...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/12026 |
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| author | Meicheng Lv Wen Luo Miaomiao Ge Yijun Guan Yan Tang Weimin Chen Jinyin Lv |
| author_facet | Meicheng Lv Wen Luo Miaomiao Ge Yijun Guan Yan Tang Weimin Chen Jinyin Lv |
| author_sort | Meicheng Lv |
| collection | DOAJ |
| description | WRKYs are one of the largest transcription factor (TF) families and play an important role in plant resistance to various stresses. TaWRKY133, a group I WRKY protein, responds to a variety of abiotic stresses, including PEG treatment. The TaWRKY133 protein is located in the nucleus of tobacco epidermal cells, and both its N-terminal and C-terminal domains exhibit transcriptional activation activity. Overexpression of <i>TaWRKY133</i> reduced drought tolerance in <i>Arabidopsis thaliana</i>, as reflected by a lower germination rate, shorter roots, higher stomatal aperture, poorer growth and lower antioxidant enzyme activities under drought treatment. Moreover, expression levels of stress-related genes (<i>DREB2A</i>, <i>RD29A</i>, <i>RD29B</i>, <i>ABF1</i>, <i>ABA2</i>, <i>ABI1</i>, <i>SOD (Cu/Zn)</i>, <i>POD1</i> and <i>CAT1</i>) were downregulated in transgenic <i>Arabidopsis</i> under drought stress. Gene silencing of <i>TaWRKY133</i> enhanced the drought tolerance of wheat, as reflected in better growth, higher antioxidant enzyme activities, and higher expression levels of stress-related genes including <i>DREB1</i>, <i>DREB3</i>, <i>ABF</i>, <i>ERF3</i>, <i>SOD (Fe)</i>, <i>POD</i>, <i>CAT</i> and <i>P5CS</i>. In conclusion, these results suggest that <i>TaWRKY133</i> might reduce drought tolerance in plants by regulating the expression of stress-related genes. |
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| last_indexed | 2024-03-09T21:36:38Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c0d1114cf7fd4a70bdc368da1b07a6d32023-11-23T20:43:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191202610.3390/ijms231912026A Group I WRKY Gene, <i>TaWRKY133</i>, Negatively Regulates Drought Resistance in Transgenic PlantsMeicheng Lv0Wen Luo1Miaomiao Ge2Yijun Guan3Yan Tang4Weimin Chen5Jinyin Lv6College of Life Sciences, Northwest A&F University, Yangling 712100, ChinaCollege of Life Sciences, Northwest A&F University, Yangling 712100, ChinaCollege of Life Sciences, Northwest A&F University, Yangling 712100, ChinaCollege of Life Sciences, Northwest A&F University, Yangling 712100, ChinaCollege of Life Sciences, Northwest A&F University, Yangling 712100, ChinaCollege of Life Sciences, Northwest A&F University, Yangling 712100, ChinaCollege of Life Sciences, Northwest A&F University, Yangling 712100, ChinaWRKYs are one of the largest transcription factor (TF) families and play an important role in plant resistance to various stresses. TaWRKY133, a group I WRKY protein, responds to a variety of abiotic stresses, including PEG treatment. The TaWRKY133 protein is located in the nucleus of tobacco epidermal cells, and both its N-terminal and C-terminal domains exhibit transcriptional activation activity. Overexpression of <i>TaWRKY133</i> reduced drought tolerance in <i>Arabidopsis thaliana</i>, as reflected by a lower germination rate, shorter roots, higher stomatal aperture, poorer growth and lower antioxidant enzyme activities under drought treatment. Moreover, expression levels of stress-related genes (<i>DREB2A</i>, <i>RD29A</i>, <i>RD29B</i>, <i>ABF1</i>, <i>ABA2</i>, <i>ABI1</i>, <i>SOD (Cu/Zn)</i>, <i>POD1</i> and <i>CAT1</i>) were downregulated in transgenic <i>Arabidopsis</i> under drought stress. Gene silencing of <i>TaWRKY133</i> enhanced the drought tolerance of wheat, as reflected in better growth, higher antioxidant enzyme activities, and higher expression levels of stress-related genes including <i>DREB1</i>, <i>DREB3</i>, <i>ABF</i>, <i>ERF3</i>, <i>SOD (Fe)</i>, <i>POD</i>, <i>CAT</i> and <i>P5CS</i>. In conclusion, these results suggest that <i>TaWRKY133</i> might reduce drought tolerance in plants by regulating the expression of stress-related genes.https://www.mdpi.com/1422-0067/23/19/12026WRKYwheatdrought stressoverexpressionVIGS |
| spellingShingle | Meicheng Lv Wen Luo Miaomiao Ge Yijun Guan Yan Tang Weimin Chen Jinyin Lv A Group I WRKY Gene, <i>TaWRKY133</i>, Negatively Regulates Drought Resistance in Transgenic Plants International Journal of Molecular Sciences WRKY wheat drought stress overexpression VIGS |
| title | A Group I WRKY Gene, <i>TaWRKY133</i>, Negatively Regulates Drought Resistance in Transgenic Plants |
| title_full | A Group I WRKY Gene, <i>TaWRKY133</i>, Negatively Regulates Drought Resistance in Transgenic Plants |
| title_fullStr | A Group I WRKY Gene, <i>TaWRKY133</i>, Negatively Regulates Drought Resistance in Transgenic Plants |
| title_full_unstemmed | A Group I WRKY Gene, <i>TaWRKY133</i>, Negatively Regulates Drought Resistance in Transgenic Plants |
| title_short | A Group I WRKY Gene, <i>TaWRKY133</i>, Negatively Regulates Drought Resistance in Transgenic Plants |
| title_sort | group i wrky gene i tawrky133 i negatively regulates drought resistance in transgenic plants |
| topic | WRKY wheat drought stress overexpression VIGS |
| url | https://www.mdpi.com/1422-0067/23/19/12026 |
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