Alterations in the gut microbiome and metabolome profiles of septic mice treated with Shen FuHuang formula
Sepsis has a high mortality rate, and treating sepsis remains a significant challenge worldwide. In former studies, our group found that traditional Chinese medicine, Shen FuHuang formula (SFH), is a promising medicine in treating coronavirus disease 2019 (COVID-19) patients with the septic syndrome...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1111962/full |
| _version_ | 1811158402286485504 |
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| author | Shasha He Shasha He Shasha He Chunxia Zhao Chunxia Zhao Chunxia Zhao Yuhong Guo Yuhong Guo Yuhong Guo Jingxia Zhao Jingxia Zhao Jingxia Zhao Xiaolong Xu Xiaolong Xu Xiaolong Xu Yahui Hu Yahui Hu Yahui Hu Bo Lian Haoran Ye Haoran Ye Haoran Ye Ning Wang Ning Wang Lianxiang Luo Qingquan Liu Qingquan Liu Qingquan Liu |
| author_facet | Shasha He Shasha He Shasha He Chunxia Zhao Chunxia Zhao Chunxia Zhao Yuhong Guo Yuhong Guo Yuhong Guo Jingxia Zhao Jingxia Zhao Jingxia Zhao Xiaolong Xu Xiaolong Xu Xiaolong Xu Yahui Hu Yahui Hu Yahui Hu Bo Lian Haoran Ye Haoran Ye Haoran Ye Ning Wang Ning Wang Lianxiang Luo Qingquan Liu Qingquan Liu Qingquan Liu |
| author_sort | Shasha He |
| collection | DOAJ |
| description | Sepsis has a high mortality rate, and treating sepsis remains a significant challenge worldwide. In former studies, our group found that traditional Chinese medicine, Shen FuHuang formula (SFH), is a promising medicine in treating coronavirus disease 2019 (COVID-19) patients with the septic syndrome. However, the underlying mechanisms remain elusive. In the present study, we first investigated the therapeutic effects of SFH on septic mice. To investigate the mechanisms of SFH-treated sepsis, we identified the gut microbiome profile and exploited untargeted metabolomics analyses. The results demonstrated that SFH significantly enhanced the mice’s 7-day survival rate and hindered the release of inflammatory mediators, i.e., TNF-α, IL-6, and IL-1β. 16S rDNA sequencing further deciphered that SFH decreased the proportion of Campylobacterota and Proteobacteria at the phylum level. LEfSe analysis revealed that the treatment of SFH enriched Blautia while decreased Escherichia_Shigella. Furthermore, serum untargeted metabolomics analysis indicated that SFH could regulate the glucagon signaling pathway, PPAR signaling pathway, galactose metabolism, and pyrimidine metabolism. Finally, we found the relative abundance of Bacteroides, Lachnospiraceae_NK4A136_group, Escherichia_Shigella, Blautia, Ruminococcus, and Prevotella were closely related to the enrichment of the metabolic signaling pathways, including L-tryptophan, uracil, glucuronic acid, protocatechuic acid, and gamma-Glutamylcysteine. In conclusion, our study demonstrated that SFH alleviated sepsis by suppressing the inflammatory response and hence reduced mortality. The mechanism of SFH for treating sepsis may be ascribed to the enrichment of beneficial gut flora and modulation in glucagon signaling pathway, PPAR signaling pathway, galactose metabolism, and pyrimidine metabolism. To sum up, these findings provide a new scientific perspective for the clinical application of SFH in treating sepsis. |
| first_indexed | 2024-04-10T05:22:49Z |
| format | Article |
| id | doaj.art-c0d28b30058644a492b049d734ea1077 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T05:22:49Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c0d28b30058644a492b049d734ea10772023-03-08T05:46:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.11119621111962Alterations in the gut microbiome and metabolome profiles of septic mice treated with Shen FuHuang formulaShasha He0Shasha He1Shasha He2Chunxia Zhao3Chunxia Zhao4Chunxia Zhao5Yuhong Guo6Yuhong Guo7Yuhong Guo8Jingxia Zhao9Jingxia Zhao10Jingxia Zhao11Xiaolong Xu12Xiaolong Xu13Xiaolong Xu14Yahui Hu15Yahui Hu16Yahui Hu17Bo Lian18Haoran Ye19Haoran Ye20Haoran Ye21Ning Wang22Ning Wang23Lianxiang Luo24Qingquan Liu25Qingquan Liu26Qingquan Liu27Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaBeijing Institute of Chinese Medicine, Beijing, ChinaBeijing Key Laboratory of Basic Research with Traditional Chinese Medicine on Infectious Diseases, Beijing, ChinaBeijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaBeijing Institute of Chinese Medicine, Beijing, ChinaBeijing Key Laboratory of Basic Research with Traditional Chinese Medicine on Infectious Diseases, Beijing, ChinaBeijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaBeijing Institute of Chinese Medicine, Beijing, ChinaBeijing Key Laboratory of Basic Research with Traditional Chinese Medicine on Infectious Diseases, Beijing, ChinaBeijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaBeijing Institute of Chinese Medicine, Beijing, ChinaBeijing Key Laboratory of Basic Research with Traditional Chinese Medicine on Infectious Diseases, Beijing, ChinaBeijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaBeijing Institute of Chinese Medicine, Beijing, ChinaBeijing Key Laboratory of Basic Research with Traditional Chinese Medicine on Infectious Diseases, Beijing, ChinaBeijing Institute of Chinese Medicine, Beijing, ChinaBeijing Key Laboratory of Basic Research with Traditional Chinese Medicine on Infectious Diseases, Beijing, ChinaTianjin University of Traditional Chinese Medicine, Tianjin, ChinaBeijing Chaoyang Hospital, Capital Medical University, Beijing, ChinaBeijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaBeijing Institute of Chinese Medicine, Beijing, ChinaBeijing Key Laboratory of Basic Research with Traditional Chinese Medicine on Infectious Diseases, Beijing, ChinaBeijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaBeijing Institute of Chinese Medicine, Beijing, ChinaThe Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang, Guangdong, ChinaBeijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaBeijing Institute of Chinese Medicine, Beijing, ChinaBeijing Key Laboratory of Basic Research with Traditional Chinese Medicine on Infectious Diseases, Beijing, ChinaSepsis has a high mortality rate, and treating sepsis remains a significant challenge worldwide. In former studies, our group found that traditional Chinese medicine, Shen FuHuang formula (SFH), is a promising medicine in treating coronavirus disease 2019 (COVID-19) patients with the septic syndrome. However, the underlying mechanisms remain elusive. In the present study, we first investigated the therapeutic effects of SFH on septic mice. To investigate the mechanisms of SFH-treated sepsis, we identified the gut microbiome profile and exploited untargeted metabolomics analyses. The results demonstrated that SFH significantly enhanced the mice’s 7-day survival rate and hindered the release of inflammatory mediators, i.e., TNF-α, IL-6, and IL-1β. 16S rDNA sequencing further deciphered that SFH decreased the proportion of Campylobacterota and Proteobacteria at the phylum level. LEfSe analysis revealed that the treatment of SFH enriched Blautia while decreased Escherichia_Shigella. Furthermore, serum untargeted metabolomics analysis indicated that SFH could regulate the glucagon signaling pathway, PPAR signaling pathway, galactose metabolism, and pyrimidine metabolism. Finally, we found the relative abundance of Bacteroides, Lachnospiraceae_NK4A136_group, Escherichia_Shigella, Blautia, Ruminococcus, and Prevotella were closely related to the enrichment of the metabolic signaling pathways, including L-tryptophan, uracil, glucuronic acid, protocatechuic acid, and gamma-Glutamylcysteine. In conclusion, our study demonstrated that SFH alleviated sepsis by suppressing the inflammatory response and hence reduced mortality. The mechanism of SFH for treating sepsis may be ascribed to the enrichment of beneficial gut flora and modulation in glucagon signaling pathway, PPAR signaling pathway, galactose metabolism, and pyrimidine metabolism. To sum up, these findings provide a new scientific perspective for the clinical application of SFH in treating sepsis.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1111962/fullsepsistraditional Chinese medicineShen FuHuang formulagut microbiomemetabonomics |
| spellingShingle | Shasha He Shasha He Shasha He Chunxia Zhao Chunxia Zhao Chunxia Zhao Yuhong Guo Yuhong Guo Yuhong Guo Jingxia Zhao Jingxia Zhao Jingxia Zhao Xiaolong Xu Xiaolong Xu Xiaolong Xu Yahui Hu Yahui Hu Yahui Hu Bo Lian Haoran Ye Haoran Ye Haoran Ye Ning Wang Ning Wang Lianxiang Luo Qingquan Liu Qingquan Liu Qingquan Liu Alterations in the gut microbiome and metabolome profiles of septic mice treated with Shen FuHuang formula Frontiers in Microbiology sepsis traditional Chinese medicine Shen FuHuang formula gut microbiome metabonomics |
| title | Alterations in the gut microbiome and metabolome profiles of septic mice treated with Shen FuHuang formula |
| title_full | Alterations in the gut microbiome and metabolome profiles of septic mice treated with Shen FuHuang formula |
| title_fullStr | Alterations in the gut microbiome and metabolome profiles of septic mice treated with Shen FuHuang formula |
| title_full_unstemmed | Alterations in the gut microbiome and metabolome profiles of septic mice treated with Shen FuHuang formula |
| title_short | Alterations in the gut microbiome and metabolome profiles of septic mice treated with Shen FuHuang formula |
| title_sort | alterations in the gut microbiome and metabolome profiles of septic mice treated with shen fuhuang formula |
| topic | sepsis traditional Chinese medicine Shen FuHuang formula gut microbiome metabonomics |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1111962/full |
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