Identification, molecular characterization and expression of JAZ genes in Lycoris aurea.
Jasmonates (JAs) are key phytohormones involved in regulation of plant growth and development, stress responses, and secondary metabolism. It has been reported that treatments with JAs could increase the contents of Amaryllidaceae alkaloids in Amaryllidaceae plants. Jasmonate ZIM (zinc-finger inflor...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0230177 |
| _version_ | 1818581359394816000 |
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| author | Peng Wang Shuojun Yu Xiaokang Han Junya Xu Qingyuan He Sheng Xu Ren Wang |
| author_facet | Peng Wang Shuojun Yu Xiaokang Han Junya Xu Qingyuan He Sheng Xu Ren Wang |
| author_sort | Peng Wang |
| collection | DOAJ |
| description | Jasmonates (JAs) are key phytohormones involved in regulation of plant growth and development, stress responses, and secondary metabolism. It has been reported that treatments with JAs could increase the contents of Amaryllidaceae alkaloids in Amaryllidaceae plants. Jasmonate ZIM (zinc-finger inflorescence meristem) domain (JAZ) proteins are key components in JA signal processes. However, JAZ proteins have not been characterized in genus Lycoris. In this study, we identified and cloned seven differentially expressed JAZ genes (namely LaJAZ1-LaJAZ7) from Lycoris aurea. Bioinformatic analyses revealed that these seven LaJAZ proteins contain the ZIM domain and JA-associated (Jas, also named CCT_2) motif. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed that these LaJAZ genes display different expression patterns in L. aurea tissues, and most of them are inducible when treated with methyl jasmonate (MeJA) treatment. Subcellular localization assay demonstrated that LaJAZ proteins are localized in the cell nucleus or cytoplasm. In addition, LaJAZ proteins could interact with each other to form homodimer and/or heterodimer. The findings in this study may facilitate further functional research of the LaJAZ genes, especially the potential regulatory mechanism of plant secondary metabolites including Amaryllidaceae alkaloids in L. aurea. |
| first_indexed | 2024-12-16T07:32:14Z |
| format | Article |
| id | doaj.art-c0d303f1212941f1a4107fc8a6e83867 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T07:32:14Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c0d303f1212941f1a4107fc8a6e838672022-12-21T22:39:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01153e023017710.1371/journal.pone.0230177Identification, molecular characterization and expression of JAZ genes in Lycoris aurea.Peng WangShuojun YuXiaokang HanJunya XuQingyuan HeSheng XuRen WangJasmonates (JAs) are key phytohormones involved in regulation of plant growth and development, stress responses, and secondary metabolism. It has been reported that treatments with JAs could increase the contents of Amaryllidaceae alkaloids in Amaryllidaceae plants. Jasmonate ZIM (zinc-finger inflorescence meristem) domain (JAZ) proteins are key components in JA signal processes. However, JAZ proteins have not been characterized in genus Lycoris. In this study, we identified and cloned seven differentially expressed JAZ genes (namely LaJAZ1-LaJAZ7) from Lycoris aurea. Bioinformatic analyses revealed that these seven LaJAZ proteins contain the ZIM domain and JA-associated (Jas, also named CCT_2) motif. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed that these LaJAZ genes display different expression patterns in L. aurea tissues, and most of them are inducible when treated with methyl jasmonate (MeJA) treatment. Subcellular localization assay demonstrated that LaJAZ proteins are localized in the cell nucleus or cytoplasm. In addition, LaJAZ proteins could interact with each other to form homodimer and/or heterodimer. The findings in this study may facilitate further functional research of the LaJAZ genes, especially the potential regulatory mechanism of plant secondary metabolites including Amaryllidaceae alkaloids in L. aurea.https://doi.org/10.1371/journal.pone.0230177 |
| spellingShingle | Peng Wang Shuojun Yu Xiaokang Han Junya Xu Qingyuan He Sheng Xu Ren Wang Identification, molecular characterization and expression of JAZ genes in Lycoris aurea. PLoS ONE |
| title | Identification, molecular characterization and expression of JAZ genes in Lycoris aurea. |
| title_full | Identification, molecular characterization and expression of JAZ genes in Lycoris aurea. |
| title_fullStr | Identification, molecular characterization and expression of JAZ genes in Lycoris aurea. |
| title_full_unstemmed | Identification, molecular characterization and expression of JAZ genes in Lycoris aurea. |
| title_short | Identification, molecular characterization and expression of JAZ genes in Lycoris aurea. |
| title_sort | identification molecular characterization and expression of jaz genes in lycoris aurea |
| url | https://doi.org/10.1371/journal.pone.0230177 |
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