The Species-Level Composition of the Fecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Genera in Indonesian Children Differs from That of Their Mothers
The infant gut microbiota plays a critical role in early life growth and derives mainly from maternal gut and breast milk. This study aimed to analyze the differences in the gut microbiota, namely <i>Bifidobacterium</i> and <i>Lactobacillus</i> communities at species level am...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/9/1995 |
| _version_ | 1797518101178220544 |
|---|---|
| author | Mengfan Ding Bo Yang Wei Wei Thwe Khine Yuan-Kun Lee Endang Sutriswati Rahayu R. Paul Ross Catherine Stanton Jianxin Zhao Hao Zhang Wei Chen |
| author_facet | Mengfan Ding Bo Yang Wei Wei Thwe Khine Yuan-Kun Lee Endang Sutriswati Rahayu R. Paul Ross Catherine Stanton Jianxin Zhao Hao Zhang Wei Chen |
| author_sort | Mengfan Ding |
| collection | DOAJ |
| description | The infant gut microbiota plays a critical role in early life growth and derives mainly from maternal gut and breast milk. This study aimed to analyze the differences in the gut microbiota, namely <i>Bifidobacterium</i> and <i>Lactobacillus</i> communities at species level among breast milk as well as maternal and infant feces at different time points after delivery. Fifty-one mother–infant pairs from Indonesia were recruited, and the breast milk and maternal and infant feces were collected and analyzed by high throughput sequencing (16S rRNA, <i>Bifidobacterium</i> groEL and <i>Lactobacillus</i> groEL genes). PCoA results showed bacterial composition was different among breast milk and maternal and infant feces within the first two years. The abundance of <i>Bifidobacterium</i> and <i>Bacteroides</i> were significantly higher in infant feces compared to their maternal feces from birth to two years of age, and maternal breast milk within six months after birth (<i>p <</i> 0.05), whereas the abundance of <i>Blautia</i>, <i>Prevotella</i>, and <i>Faecalibacterium</i> was higher in maternal feces compared to that in breast milk within six months and infant feces within one year after birth, respectively (<i>p <</i> 0.05). The relative abundances of <i>Bacteroides</i> and <i>Lactobacillus</i> was higher and lower in infant feces compared to that in maternal feces only between one and two years of age, respectively (<i>p <</i> 0.05). For <i>Bifidobacterium</i> community at species level, <i>B. adolescentis, B. ruminantium, B. longum subsp. infantis, B. bifidum,</i> and <i>B. pseudolongum</i> were identified in all samples. However, the profile of <i>Bifidobacterium</i> was different between maternal and infant feces at different ages. The relative abundances of <i>B. adolescentis</i> and <i>B. ruminantium</i> were higher in maternal feces compared to those in infant feces from birth to one year of age (<i>p <</i> 0.05), while the relative abundances of <i>B. longum</i> subsp. <i>infantis</i> and <i>B. bifidum</i> were higher in infant feces compared to those in maternal feces beyond three months, and the relative abundance of <i>B. pseudolongum</i> was only higher in infant feces between three and six months (<i>p <</i> 0.05). For <i>Lactobacillus</i> community, <i>L. paragasseri</i> showed higher relative abundance in infant feces when the infant was younger than one year of age (<i>p <</i> 0.05). This study showed bacterial composition at the genus level and <i>Bifidobacterium</i> and <i>Lactobacillus</i> communities at the species level were stage specific in maternal breast milk as well as and maternal and infant feces. |
| first_indexed | 2024-03-10T07:25:26Z |
| format | Article |
| id | doaj.art-c0d74639f5664f2ea762b53799e467b1 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:25:26Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c0d74639f5664f2ea762b53799e467b12023-11-22T14:20:17ZengMDPI AGMicroorganisms2076-26072021-09-0199199510.3390/microorganisms9091995The Species-Level Composition of the Fecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Genera in Indonesian Children Differs from That of Their MothersMengfan Ding0Bo Yang1Wei Wei Thwe Khine2Yuan-Kun Lee3Endang Sutriswati Rahayu4R. Paul Ross5Catherine Stanton6Jianxin Zhao7Hao Zhang8Wei Chen9State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaDepartment of Microbiology & Immunology, National University of Singapore, Singapore 117545, SingaporeInternational Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, ChinaDepartment of Food and Agricultural Product Technology, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaInternational Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, ChinaInternational Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaThe infant gut microbiota plays a critical role in early life growth and derives mainly from maternal gut and breast milk. This study aimed to analyze the differences in the gut microbiota, namely <i>Bifidobacterium</i> and <i>Lactobacillus</i> communities at species level among breast milk as well as maternal and infant feces at different time points after delivery. Fifty-one mother–infant pairs from Indonesia were recruited, and the breast milk and maternal and infant feces were collected and analyzed by high throughput sequencing (16S rRNA, <i>Bifidobacterium</i> groEL and <i>Lactobacillus</i> groEL genes). PCoA results showed bacterial composition was different among breast milk and maternal and infant feces within the first two years. The abundance of <i>Bifidobacterium</i> and <i>Bacteroides</i> were significantly higher in infant feces compared to their maternal feces from birth to two years of age, and maternal breast milk within six months after birth (<i>p <</i> 0.05), whereas the abundance of <i>Blautia</i>, <i>Prevotella</i>, and <i>Faecalibacterium</i> was higher in maternal feces compared to that in breast milk within six months and infant feces within one year after birth, respectively (<i>p <</i> 0.05). The relative abundances of <i>Bacteroides</i> and <i>Lactobacillus</i> was higher and lower in infant feces compared to that in maternal feces only between one and two years of age, respectively (<i>p <</i> 0.05). For <i>Bifidobacterium</i> community at species level, <i>B. adolescentis, B. ruminantium, B. longum subsp. infantis, B. bifidum,</i> and <i>B. pseudolongum</i> were identified in all samples. However, the profile of <i>Bifidobacterium</i> was different between maternal and infant feces at different ages. The relative abundances of <i>B. adolescentis</i> and <i>B. ruminantium</i> were higher in maternal feces compared to those in infant feces from birth to one year of age (<i>p <</i> 0.05), while the relative abundances of <i>B. longum</i> subsp. <i>infantis</i> and <i>B. bifidum</i> were higher in infant feces compared to those in maternal feces beyond three months, and the relative abundance of <i>B. pseudolongum</i> was only higher in infant feces between three and six months (<i>p <</i> 0.05). For <i>Lactobacillus</i> community, <i>L. paragasseri</i> showed higher relative abundance in infant feces when the infant was younger than one year of age (<i>p <</i> 0.05). This study showed bacterial composition at the genus level and <i>Bifidobacterium</i> and <i>Lactobacillus</i> communities at the species level were stage specific in maternal breast milk as well as and maternal and infant feces.https://www.mdpi.com/2076-2607/9/9/1995human breast milkmaternal fecesinfant feces<i>Bifidobacterium</i> community<i>Lactobacillus</i> community |
| spellingShingle | Mengfan Ding Bo Yang Wei Wei Thwe Khine Yuan-Kun Lee Endang Sutriswati Rahayu R. Paul Ross Catherine Stanton Jianxin Zhao Hao Zhang Wei Chen The Species-Level Composition of the Fecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Genera in Indonesian Children Differs from That of Their Mothers Microorganisms human breast milk maternal feces infant feces <i>Bifidobacterium</i> community <i>Lactobacillus</i> community |
| title | The Species-Level Composition of the Fecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Genera in Indonesian Children Differs from That of Their Mothers |
| title_full | The Species-Level Composition of the Fecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Genera in Indonesian Children Differs from That of Their Mothers |
| title_fullStr | The Species-Level Composition of the Fecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Genera in Indonesian Children Differs from That of Their Mothers |
| title_full_unstemmed | The Species-Level Composition of the Fecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Genera in Indonesian Children Differs from That of Their Mothers |
| title_short | The Species-Level Composition of the Fecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Genera in Indonesian Children Differs from That of Their Mothers |
| title_sort | species level composition of the fecal i bifidobacterium i and i lactobacillus i genera in indonesian children differs from that of their mothers |
| topic | human breast milk maternal feces infant feces <i>Bifidobacterium</i> community <i>Lactobacillus</i> community |
| url | https://www.mdpi.com/2076-2607/9/9/1995 |
| work_keys_str_mv | AT mengfanding thespecieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT boyang thespecieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT weiweithwekhine thespecieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT yuankunlee thespecieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT endangsutriswatirahayu thespecieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT rpaulross thespecieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT catherinestanton thespecieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT jianxinzhao thespecieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT haozhang thespecieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT weichen thespecieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT mengfanding specieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT boyang specieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT weiweithwekhine specieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT yuankunlee specieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT endangsutriswatirahayu specieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT rpaulross specieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT catherinestanton specieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT jianxinzhao specieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT haozhang specieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers AT weichen specieslevelcompositionofthefecalibifidobacteriumiandilactobacillusigenerainindonesianchildrendiffersfromthatoftheirmothers |