Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents
Current cysteine bioconjugation strategies for protein-drug conjugates synthesis often yield heterogeneous and poorly stable products. Here, the authors use carbonylacrylic derivatives to selectively modify cysteine residues and synthesize biologically functional antibody conjugates highly stable in...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2016-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms13128 |
| _version_ | 1819040345273401344 |
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| author | Barbara Bernardim Pedro M.S.D. Cal Maria J. Matos Bruno L. Oliveira Nuria Martínez-Sáez Inês S. Albuquerque Elizabeth Perkins Francisco Corzana Antonio C.B. Burtoloso Gonzalo Jiménez-Osés Gonçalo J. L. Bernardes |
| author_facet | Barbara Bernardim Pedro M.S.D. Cal Maria J. Matos Bruno L. Oliveira Nuria Martínez-Sáez Inês S. Albuquerque Elizabeth Perkins Francisco Corzana Antonio C.B. Burtoloso Gonzalo Jiménez-Osés Gonçalo J. L. Bernardes |
| author_sort | Barbara Bernardim |
| collection | DOAJ |
| description | Current cysteine bioconjugation strategies for protein-drug conjugates synthesis often yield heterogeneous and poorly stable products. Here, the authors use carbonylacrylic derivatives to selectively modify cysteine residues and synthesize biologically functional antibody conjugates highly stable in plasma. |
| first_indexed | 2024-12-21T09:07:37Z |
| format | Article |
| id | doaj.art-c0d7ebdccc9c4ce596735356cd570e4c |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-21T09:07:37Z |
| publishDate | 2016-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-c0d7ebdccc9c4ce596735356cd570e4c2022-12-21T19:09:19ZengNature PortfolioNature Communications2041-17232016-10-01711910.1038/ncomms13128Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagentsBarbara Bernardim0Pedro M.S.D. Cal1Maria J. Matos2Bruno L. Oliveira3Nuria Martínez-Sáez4Inês S. Albuquerque5Elizabeth Perkins6Francisco Corzana7Antonio C.B. Burtoloso8Gonzalo Jiménez-Osés9Gonçalo J. L. Bernardes10Department of Chemistry, University of CambridgeDepartment of Chemistry, University of CambridgeDepartment of Chemistry, University of CambridgeDepartment of Chemistry, University of CambridgeDepartment of Chemistry, University of CambridgeInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de LisboaAlbumedix Ltd, Castle CourtDepartment of Chemistry, University of CambridgeInstituto de Química de São Carlos, Universidade de São PauloDepartamento de Química, Universidad de La Rioja, Centro de Investigacioón en Síntesis QuímicaDepartment of Chemistry, University of CambridgeCurrent cysteine bioconjugation strategies for protein-drug conjugates synthesis often yield heterogeneous and poorly stable products. Here, the authors use carbonylacrylic derivatives to selectively modify cysteine residues and synthesize biologically functional antibody conjugates highly stable in plasma.https://doi.org/10.1038/ncomms13128 |
| spellingShingle | Barbara Bernardim Pedro M.S.D. Cal Maria J. Matos Bruno L. Oliveira Nuria Martínez-Sáez Inês S. Albuquerque Elizabeth Perkins Francisco Corzana Antonio C.B. Burtoloso Gonzalo Jiménez-Osés Gonçalo J. L. Bernardes Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents Nature Communications |
| title | Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents |
| title_full | Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents |
| title_fullStr | Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents |
| title_full_unstemmed | Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents |
| title_short | Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents |
| title_sort | stoichiometric and irreversible cysteine selective protein modification using carbonylacrylic reagents |
| url | https://doi.org/10.1038/ncomms13128 |
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