Enhanced Ohmyungsamycin A Production via Adenylation Domain Engineering and Optimization of Culture Conditions

Enhanced Ohmyungsamycin A Production via Adenylation Domain Engineering and Optimization of Culture Conditions

Ohmyungsamycins (OMSs) A and B are cyclic depsipeptides produced by marine Streptomyces strains, which are synthesized by a non-ribosomal peptide synthetase. Notably, OMS A exhibits more potent activity against Mycobacterium tuberculosis and human cancer cells than OMS B. The substrate promiscuous a...

Full description

Bibliographic Details
Main Authors:	Eunji Kim, Young Eun Du, Yeon Hee Ban, Yern-Hyerk Shin, Dong-Chan Oh, Yeo Joon Yoon
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2021-02-01
Series:	Frontiers in Microbiology
Subjects:	ohmyungsamycin non-ribosomal peptide synthetase adenylation domain engineering site-directed mutagenesis culture condition optimization
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2021.626881/full

Similar Items

Characterization of the Ohmyungsamycin Biosynthetic Pathway and Generation of Derivatives with Improved Antituberculosis Activity
by: Eunji Kim, et al.
Published: (2019-10-01)

Structures of teixobactin-producing nonribosomal peptide synthetase condensation and adenylation domains
by: Kemin Tan, et al.
Published: (2020-01-01)

Discovery of unguisin J, a new cyclic peptide from Aspergillus heteromorphus CBS 117.55, and phylogeny-based bioinformatic analysis of UngA NRPS domains
by: Sharmila Neupane, et al.
Published: (2024-02-01)

A non-canonical peptide synthetase adenylates 3-methyl-2-oxovaleric acid for auriculamide biosynthesis
by: Daniel Braga, et al.
Published: (2016-12-01)

Development of a chemical scaffold for inhibiting nonribosomal peptide synthetases in live bacterial cells
by: Fumihiro Ishikawa, et al.
Published: (2024-02-01)

Ohmyungsamycin promotes M1-like inflammatory responses to enhance host defence against Mycobacteroides abscessus infections
by: Sang Min Jeon, et al.
Published: (2022-12-01)

A Cysteine-Reloading Process Initiating the Biosynthesis of the Bicyclic Scaffold of Dithiolopyrrolones
by: Yan Chen, et al.
Published: (2023-04-01)

Genomics-driven discovery of a biosynthetic gene cluster required for the synthesis of BII-Rafflesfungin from the fungus Phoma sp. F3723
by: Swati Sinha, et al.
Published: (2019-05-01)

Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase
by: Fuchao Xu, et al.
Published: (2019-07-01)

Phylogenetic Study of Polyketide Synthases and Nonribosomal Peptide Synthetases Involved in the Biosynthesis of Mycotoxins
by: Massimo Ferrara, et al.
Published: (2013-04-01)

Repurposing Modular Polyketide Synthases and Non-ribosomal Peptide Synthetases for Novel Chemical Biosynthesis
by: Soonkyu Hwang, et al.
Published: (2020-05-01)

The Biosynthesis of Rare Homo-Amino Acid Containing Variants of Microcystin by a Benthic Cyanobacterium
by: Tânia Keiko Shishido, et al.
Published: (2019-05-01)

Molecular dynamics simulation studies of novel marine psychrophilic adenylate kinase with mesophilic and thermophilic homologous /
by: Yusra Nazira Mohd. Yusop, 1991, author, et al.
Published: (2014)

Molecular dynamics simulation studies of novel marine psychrophilic adenylate kinase with mesophilic and thermophilic homologous [electronic resource] /
by: Yusra Nazira Mohd. Yusop, 1991, author
Published: (2014)

Molecular, chemical and biological screening of soil actinomycete isolates in seeking bioactive peptide metabolites
by: Javad Hamedi, et al.
Published: (2015-10-01)

The inherent flexibility of type I non-ribosomal peptide synthetase multienzymes drives their catalytic activities
by: Sarah Bonhomme, et al.
Published: (2021-05-01)

Bordetella Pertussis Adenylate Cyclase Toxin Does Not Possess a Phospholipase A Activity; Serine 606 and Aspartate 1079 Residues Are Not Involved in Target Cell Delivery of the Adenylyl Cyclase Enzyme Domain
by: Ladislav Bumba, et al.
Published: (2018-06-01)

Bordetella Adenylate Cyclase-Hemolysin Toxins
by: Nicole Guiso
Published: (2017-09-01)

An accurate strategy for pointing the key biocatalytic sites of bre2691A protein for modification of the brevilaterin from Brevibacillus laterosporus
by: Panpan Han, et al.
Published: (2022-09-01)

Adenylate Kinase and Metabolic Signaling in Cancer Cells
by: Aleksandr Klepinin, et al.
Published: (2020-05-01)

Characterization and Molecular Mechanism of Aminoglycoside-6-Adenyl Transferase Associated with Aminoglycoside Resistance from Elizabethkingia meningoseptica
by: Zhang S, et al.
Published: (2023-08-01)

Complex peptide natural products: Biosynthetic principles, challenges and opportunities for pathway engineering
by: Sebastian L. Wenski, et al.
Published: (2022-03-01)

Antitumor Compounds from Marine Actinomycetes
by: José A. Salas, et al.
Published: (2009-06-01)

Adenylate Cyclase Toxin Tinkering With Monocyte-Macrophage Differentiation
by: Jawid Nazir Ahmad, et al.
Published: (2020-09-01)

Microbial Lipopeptide-Producing Strains and Their Metabolic Roles under Anaerobic Conditions
by: Jia-Yi Li, et al.
Published: (2021-09-01)

Ca2+-dependent adenylate cyclase of plant cells in bacterial pathogenesis
by: A.S. Romanenko, et al.
Published: (2019-06-01)

Determination of Adenylate Nucleotides in Amphipod <i>Gammarus fossarum</i> by Ion-Pair Reverse Phase Liquid Chromatography: Possibilities of Positive Pressure Micro-Solid Phase Extraction
by: Zuzana Redžović, et al.
Published: (2021-02-01)

Assessing the Interactions of Statins with Human Adenylate Kinase Isoenzyme 1: Fluorescence and Enzyme Kinetic Studies
by: Magdalena Wujak, et al.
Published: (2021-05-01)

Identification and Expression Analysis of Adenylate Kinase Gene Family in Potato
by: Xiang Li, et al.
Published: (2023-09-01)

Regulation of Adenine Nucleotide Metabolism by Adenylate Kinase Isozymes: Physiological Roles and Diseases
by: Koichi Fujisawa
Published: (2023-03-01)

Optimization of ATP synthase function in mitochondria and chloroplasts via the adenylate kinase equilibrium
by: Abir U Igamberdiev, et al.
Published: (2015-01-01)

Regulation by the quorum sensor from Vibrio indicates a receptor function for the membrane anchors of adenylate cyclases
by: Stephanie Beltz, et al.
Published: (2016-02-01)

Stereomeric Lipopeptides from a Single Non-Ribosomal Peptide Synthetase as an Additional Source of Structural and Functional Diversification in <i>Pseudomonas</i> Lipopeptide Biosynthesis
by: Penthip Muangkaew, et al.
Published: (2023-09-01)

Integration of Adenylate Kinase 1 with Its Peptide Conformational Imprint
by: Cheng-Hsin Wu, et al.
Published: (2022-06-01)

Legionella shows a diverse secondary metabolism dependent on a broad spectrum Sfp-type phosphopantetheinyl transferase
by: Nicholas J. Tobias, et al.
Published: (2016-11-01)

Chemo-enzymatic total syntheses of bis-tetrahydroisoquinoline alkaloids and systematic exploration of the substrate scope of SfmC
by: Ryo Tanifuji, et al.
Published: (2022-03-01)

Aspergillus niger is a superior expression host for the production of bioactive fungal cyclodepsipeptides
by: Simon Boecker, et al.
Published: (2018-03-01)

Draft genome sequences of Cylindrospermopsis raciborskii strains CS-508 and MVCC14, isolated from freshwater bloom events in Australia and Uruguay
by: Juan J Fuentes-Valdés, et al.
Published: (2018-10-01)

Favipiravir Inhibits Hepatitis A Virus Infection in Human Hepatocytes
by: Reina Sasaki-Tanaka, et al.
Published: (2022-02-01)

Extracellular Degradation Into Adenosine and the Activities of Adenosine Kinase and AMPK Mediate Extracellular NAD+-Produced Increases in the Adenylate Pool of BV2 Microglia Under Basal Conditions
by: Jie Zhang, et al.
Published: (2018-10-01)