A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic pain
Abstract Accumulating evidence supports the notion that microglia play versatile roles in different chronic pain conditions. However, therapeutic strategies of chronic pain by targeting microglia remain largely overlooked. This study seeks to develop a miRNA-loaded nano-delivery system by targeting...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-024-02420-9 |
| _version_ | 1827310028526190592 |
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| author | Yitian Lu Shuai Liu Peng Wang Xiangna Guo Zaisheng Qin Honghao Hou Tao Tao |
| author_facet | Yitian Lu Shuai Liu Peng Wang Xiangna Guo Zaisheng Qin Honghao Hou Tao Tao |
| author_sort | Yitian Lu |
| collection | DOAJ |
| description | Abstract Accumulating evidence supports the notion that microglia play versatile roles in different chronic pain conditions. However, therapeutic strategies of chronic pain by targeting microglia remain largely overlooked. This study seeks to develop a miRNA-loaded nano-delivery system by targeting microglia, which could provide a decent and long-lasting analgesia for chronic pain. Surface aminated mesoporous silica nanoparticles were adopted to load miR-26a-5p, a potent analgesic miRNA, by electrostatic adsorption, which can avoid miR-26a-5p is rapidly released and degraded. Then, targeting peptide MG1 was modified on the surface of aminated mesoporous silica particles for microglia targeting. In peripheral nerve injury induced neuropathic pain model, a satisfactory anti-allodynia effect with about 6 weeks pain-relief duration were achieved through targeting microglia strategy, which decreased microglia activation and inflammation by Wnt5a, a non-canonical Wnt pathway. In inflammatory pain and chemotherapy induced peripheral neuropathic pain, microglia targeting strategy also exhibited more efficient analgesia and longer pain-relief duration than others. Overall, we developed a microglia-targeting nano-delivery system, which facilitates precisely miR-26a-5p delivery to enhance analgesic effect and duration for several chronic pain conditions. Graphical Abstract |
| first_indexed | 2024-04-24T19:52:05Z |
| format | Article |
| id | doaj.art-c0dae708c5db468fa729211f4ea01b6a |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-24T19:52:05Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-c0dae708c5db468fa729211f4ea01b6a2024-03-24T12:32:35ZengBMCJournal of Nanobiotechnology1477-31552024-03-0122112010.1186/s12951-024-02420-9A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic painYitian Lu0Shuai Liu1Peng Wang2Xiangna Guo3Zaisheng Qin4Honghao Hou5Tao Tao6Department of Anesthesiology, Zhujiang Hospital, Southern Medical UniversityGuangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, School of Basic Medical Sciences, Southern Medical UniversityDepartment of Anesthesiology, Nanfang Hospital, Southern Medical UniversityDepartment of Anesthesiology, Nanfang Hospital, Southern Medical UniversityDepartment of Anesthesiology, Nanfang Hospital, Southern Medical UniversityGuangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, School of Basic Medical Sciences, Southern Medical UniversityDepartment of Anesthesiology, Zhujiang Hospital, Southern Medical UniversityAbstract Accumulating evidence supports the notion that microglia play versatile roles in different chronic pain conditions. However, therapeutic strategies of chronic pain by targeting microglia remain largely overlooked. This study seeks to develop a miRNA-loaded nano-delivery system by targeting microglia, which could provide a decent and long-lasting analgesia for chronic pain. Surface aminated mesoporous silica nanoparticles were adopted to load miR-26a-5p, a potent analgesic miRNA, by electrostatic adsorption, which can avoid miR-26a-5p is rapidly released and degraded. Then, targeting peptide MG1 was modified on the surface of aminated mesoporous silica particles for microglia targeting. In peripheral nerve injury induced neuropathic pain model, a satisfactory anti-allodynia effect with about 6 weeks pain-relief duration were achieved through targeting microglia strategy, which decreased microglia activation and inflammation by Wnt5a, a non-canonical Wnt pathway. In inflammatory pain and chemotherapy induced peripheral neuropathic pain, microglia targeting strategy also exhibited more efficient analgesia and longer pain-relief duration than others. Overall, we developed a microglia-targeting nano-delivery system, which facilitates precisely miR-26a-5p delivery to enhance analgesic effect and duration for several chronic pain conditions. Graphical Abstracthttps://doi.org/10.1186/s12951-024-02420-9MG1NanoparticlesChronic painMicrogliaAnalgesia |
| spellingShingle | Yitian Lu Shuai Liu Peng Wang Xiangna Guo Zaisheng Qin Honghao Hou Tao Tao A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic pain Journal of Nanobiotechnology MG1 Nanoparticles Chronic pain Microglia Analgesia |
| title | A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic pain |
| title_full | A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic pain |
| title_fullStr | A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic pain |
| title_full_unstemmed | A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic pain |
| title_short | A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic pain |
| title_sort | novel microglia targeting strategy based on nanoparticle mediated delivery of mir 26a 5p for long lasting analgesia in chronic pain |
| topic | MG1 Nanoparticles Chronic pain Microglia Analgesia |
| url | https://doi.org/10.1186/s12951-024-02420-9 |
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