| Summary: | The biosynthesis of silver nanoparticles (AgNPs) from <i>Diospyros villosa</i> leaves and stem bark extracts is described. The stem bark AgNPs of <i>D. villosa</i> synthesized at 80 °C (S80) showed good scavenging activity with a lower IC<sub>50</sub> value of 8.75 µg·mL<sup>−1</sup> compared to ascorbic acid (9.58 µg·mL<sup>−1</sup>). The total phenol content of the S80 AgNPs was measured and found to be 10.22 ± 0.14 mg.g<sup>−1</sup> gallic acid equivalence (GAE). Bacterial growth inhibition (% GI) and violacein inhibition (% VI) of 10.08% and 58.83%, respectively, was observed against <i>C.</i><i>subtsugae</i> CV017 with leaf AgNPs synthesized at 80 °C (L80) at 80 μg·mL<sup>−1</sup>. Stem bark AgNPs synthesized at room temperature (SRT) also indicated % GI of 13.83% and % VI of 65.97% against <i>C. subtsugae</i> CV017 at 160 μg·mL<sup>−1</sup>. Leaf AgNPs of <i>D. villosa</i> synthesized at room temperature (LRT), showed % GI of 29.07% and % VI of 56.53%, respectively, against <i>C. violaceum</i> ATCC 12472 at 320 μg·mL<sup>−1</sup>. The L80 and SRT at 160 μg·mL<sup>−1</sup> and LRT at 320 μg·mL<sup>−1</sup> may be considered as potential QS inhibitors following their activity against <i>C. subtsugae</i> CV017 and <i>C. violaceum</i> ATCC 12472, respectively. Therefore, <i>D. villosa</i> represents a potential source of antioxidants as well as an anti-quorum sensing therapeutic candidate for the control of Gram-negative bacterial infections.
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