Influence of N-P-K fertilization at the acclimatization stage on micropropagated seedlings of Tillandsia bulbosa Hook

Bromeliads are known worldwide for their ornamental potential. In Brazil, species of the genus Tillandsia occur in the Atlantic rainforest, Amazon rainforest, and rocky fields. This work aimed to evaluate the influence of nitrogen, phosphorus, and potassium fertilization on micropropagated seedling...

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Main Authors: Maria de Fátima Arrigoni-Blank, Márcia Raquel Moura Vasconcelos, Rosana Barroso Feitosa-Alcantara, Maria Aparecida Moreira, Evaristo Mauro de Castro, Arie Fitzgerald Blank
Format: Article
Language:English
Published: Universidade Federal de Uberlândia 2018-05-01
Series:Bioscience Journal
Subjects:
Online Access:https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/39379
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author Maria de Fátima Arrigoni-Blank
Márcia Raquel Moura Vasconcelos
Rosana Barroso Feitosa-Alcantara
Maria Aparecida Moreira
Evaristo Mauro de Castro
Arie Fitzgerald Blank
author_facet Maria de Fátima Arrigoni-Blank
Márcia Raquel Moura Vasconcelos
Rosana Barroso Feitosa-Alcantara
Maria Aparecida Moreira
Evaristo Mauro de Castro
Arie Fitzgerald Blank
author_sort Maria de Fátima Arrigoni-Blank
collection DOAJ
description Bromeliads are known worldwide for their ornamental potential. In Brazil, species of the genus Tillandsia occur in the Atlantic rainforest, Amazon rainforest, and rocky fields. This work aimed to evaluate the influence of nitrogen, phosphorus, and potassium fertilization on micropropagated seedlings of Tillandsia bulbosa, at the acclimatization stage, and their leaf anatomy. The experiment was carried out in a completely randomized design, in a 4x4+1 factorial scheme, using coconut coir: earthworm humus: sand mixture as substrate (2:1:1). Urea, single superphosphate, and potassium chloride were used as nitrogen, phosphorus and potassium sources, respectively, at proportions of 50, 100, 200, and 400% of the dose recommended. The doses were distributed in four applications, testing total application at planting (1); ½ application at planting and ½ at 80 DAP (2); ⅓ application at planting, ⅓ at 50 DAP, and ⅓ at 100 DAP (3); and ¼ application at planting, ¼ at 30 DAP, ¼ at 60 DAP, and ¼ at 120 DAP (4); and a control (without fertilization). Leaves anatomy was analyzed at 180 days after planting. Fertilization did not significantly influence the development of seedlings during acclimatization. The doses of 50, 100, and 200% provided thicker parenchyma of chlorophyll and aquifer and leaf blade. Tillandsia bulbosa can be acclimatized without fertilizer application.
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spelling doaj.art-c0dcd45264bc4a33b335640a3a1646932022-12-21T22:49:44ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632018-05-01343Influence of N-P-K fertilization at the acclimatization stage on micropropagated seedlings of Tillandsia bulbosa Hook Maria de Fátima Arrigoni-Blank0Márcia Raquel Moura Vasconcelos1Rosana Barroso Feitosa-Alcantara2Maria Aparecida Moreira3Evaristo Mauro de Castro4Arie Fitzgerald Blank5Universidade Federal de SergipeUniversidade Federal de SergipeUniversidade Federal de SergipeUniversidade Federal de SergipeUniversidade Federal de LavrasUniversidade Federal de Sergipe Bromeliads are known worldwide for their ornamental potential. In Brazil, species of the genus Tillandsia occur in the Atlantic rainforest, Amazon rainforest, and rocky fields. This work aimed to evaluate the influence of nitrogen, phosphorus, and potassium fertilization on micropropagated seedlings of Tillandsia bulbosa, at the acclimatization stage, and their leaf anatomy. The experiment was carried out in a completely randomized design, in a 4x4+1 factorial scheme, using coconut coir: earthworm humus: sand mixture as substrate (2:1:1). Urea, single superphosphate, and potassium chloride were used as nitrogen, phosphorus and potassium sources, respectively, at proportions of 50, 100, 200, and 400% of the dose recommended. The doses were distributed in four applications, testing total application at planting (1); ½ application at planting and ½ at 80 DAP (2); â…“ application at planting, â…“ at 50 DAP, and â…“ at 100 DAP (3); and ¼ application at planting, ¼ at 30 DAP, ¼ at 60 DAP, and ¼ at 120 DAP (4); and a control (without fertilization). Leaves anatomy was analyzed at 180 days after planting. Fertilization did not significantly influence the development of seedlings during acclimatization. The doses of 50, 100, and 200% provided thicker parenchyma of chlorophyll and aquifer and leaf blade. Tillandsia bulbosa can be acclimatized without fertilizer application. https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/39379BromeliaceaeTissue cultureFertilizerLeaf anatomy
spellingShingle Maria de Fátima Arrigoni-Blank
Márcia Raquel Moura Vasconcelos
Rosana Barroso Feitosa-Alcantara
Maria Aparecida Moreira
Evaristo Mauro de Castro
Arie Fitzgerald Blank
Influence of N-P-K fertilization at the acclimatization stage on micropropagated seedlings of Tillandsia bulbosa Hook
Bioscience Journal
Bromeliaceae
Tissue culture
Fertilizer
Leaf anatomy
title Influence of N-P-K fertilization at the acclimatization stage on micropropagated seedlings of Tillandsia bulbosa Hook
title_full Influence of N-P-K fertilization at the acclimatization stage on micropropagated seedlings of Tillandsia bulbosa Hook
title_fullStr Influence of N-P-K fertilization at the acclimatization stage on micropropagated seedlings of Tillandsia bulbosa Hook
title_full_unstemmed Influence of N-P-K fertilization at the acclimatization stage on micropropagated seedlings of Tillandsia bulbosa Hook
title_short Influence of N-P-K fertilization at the acclimatization stage on micropropagated seedlings of Tillandsia bulbosa Hook
title_sort influence of n p k fertilization at the acclimatization stage on micropropagated seedlings of tillandsia bulbosa hook
topic Bromeliaceae
Tissue culture
Fertilizer
Leaf anatomy
url https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/39379
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