Dual-ligated metal organic framework as novel multifunctional nanovehicle for targeted drug delivery for hepatic cancer treatment
Abstract In the last decade, nanosized metal organic frameworks (NMOFs) have gained an increasing applicability as multifunctional nanocarriers for drug delivery in cancer therapy. However, only a limited number of platforms have been reported that can serve as an effective targeted drug delivery sy...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-99407-5 |
| _version_ | 1819036616712257536 |
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| author | Mostafa Fytory Kholoud K. Arafa Waleed M. A. El Rouby Ahmed A. Farghali Mahmoud Abdel-Hafiez Ibrahim M. El-Sherbiny |
| author_facet | Mostafa Fytory Kholoud K. Arafa Waleed M. A. El Rouby Ahmed A. Farghali Mahmoud Abdel-Hafiez Ibrahim M. El-Sherbiny |
| author_sort | Mostafa Fytory |
| collection | DOAJ |
| description | Abstract In the last decade, nanosized metal organic frameworks (NMOFs) have gained an increasing applicability as multifunctional nanocarriers for drug delivery in cancer therapy. However, only a limited number of platforms have been reported that can serve as an effective targeted drug delivery system (DDSs). Herein, we report rational design and construction of doxorubicin (DOX)-loaded nanoscale Zr (IV)-based NMOF (NH2-UiO-66) decorated with active tumor targeting moieties; folic acid (FA), lactobionic acid (LA), glycyrrhetinic acid (GA), and dual ligands of LA and GA, as efficient multifunctional DDSs for hepatocellular carcinoma (HCC) therapy. The success of modification was exhaustively validated by various structural, thermal and microscopic techniques. Biocompatibility studies indicated the safety of pristine NH2-UiO-66 against HSF cells whereas DOX-loaded dual-ligated NMOF was found to possess superior cytotoxicity against HepG2 cells which was further confirmed by flow cytometry. Moreover, fluorescence microscopy was used for monitoring cellular uptake in comparison to the non-ligated and mono-ligated NMOF. Additionally, the newly developed dual-ligated NMOF depicted a pH-responsiveness towards the DOX release. These findings open new avenues in designing various NMOF-based DDSs that actively target hepatic cancer to achieve precise therapy. |
| first_indexed | 2024-12-21T08:08:21Z |
| format | Article |
| id | doaj.art-c0dd6fd376464b3e89fe47611bae9102 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T08:08:21Z |
| publishDate | 2021-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c0dd6fd376464b3e89fe47611bae91022022-12-21T19:10:45ZengNature PortfolioScientific Reports2045-23222021-10-0111111310.1038/s41598-021-99407-5Dual-ligated metal organic framework as novel multifunctional nanovehicle for targeted drug delivery for hepatic cancer treatmentMostafa Fytory0Kholoud K. Arafa1Waleed M. A. El Rouby2Ahmed A. Farghali3Mahmoud Abdel-Hafiez4Ibrahim M. El-Sherbiny5Nanomedicine Labs, Center for Materials Science (CMS), Zewail City of Science and TechnologyNanomedicine Labs, Center for Materials Science (CMS), Zewail City of Science and TechnologyMaterial Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef UniversityMaterial Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef UniversityDepartment of Physics and Astronomy, Uppsala UniversityNanomedicine Labs, Center for Materials Science (CMS), Zewail City of Science and TechnologyAbstract In the last decade, nanosized metal organic frameworks (NMOFs) have gained an increasing applicability as multifunctional nanocarriers for drug delivery in cancer therapy. However, only a limited number of platforms have been reported that can serve as an effective targeted drug delivery system (DDSs). Herein, we report rational design and construction of doxorubicin (DOX)-loaded nanoscale Zr (IV)-based NMOF (NH2-UiO-66) decorated with active tumor targeting moieties; folic acid (FA), lactobionic acid (LA), glycyrrhetinic acid (GA), and dual ligands of LA and GA, as efficient multifunctional DDSs for hepatocellular carcinoma (HCC) therapy. The success of modification was exhaustively validated by various structural, thermal and microscopic techniques. Biocompatibility studies indicated the safety of pristine NH2-UiO-66 against HSF cells whereas DOX-loaded dual-ligated NMOF was found to possess superior cytotoxicity against HepG2 cells which was further confirmed by flow cytometry. Moreover, fluorescence microscopy was used for monitoring cellular uptake in comparison to the non-ligated and mono-ligated NMOF. Additionally, the newly developed dual-ligated NMOF depicted a pH-responsiveness towards the DOX release. These findings open new avenues in designing various NMOF-based DDSs that actively target hepatic cancer to achieve precise therapy.https://doi.org/10.1038/s41598-021-99407-5 |
| spellingShingle | Mostafa Fytory Kholoud K. Arafa Waleed M. A. El Rouby Ahmed A. Farghali Mahmoud Abdel-Hafiez Ibrahim M. El-Sherbiny Dual-ligated metal organic framework as novel multifunctional nanovehicle for targeted drug delivery for hepatic cancer treatment Scientific Reports |
| title | Dual-ligated metal organic framework as novel multifunctional nanovehicle for targeted drug delivery for hepatic cancer treatment |
| title_full | Dual-ligated metal organic framework as novel multifunctional nanovehicle for targeted drug delivery for hepatic cancer treatment |
| title_fullStr | Dual-ligated metal organic framework as novel multifunctional nanovehicle for targeted drug delivery for hepatic cancer treatment |
| title_full_unstemmed | Dual-ligated metal organic framework as novel multifunctional nanovehicle for targeted drug delivery for hepatic cancer treatment |
| title_short | Dual-ligated metal organic framework as novel multifunctional nanovehicle for targeted drug delivery for hepatic cancer treatment |
| title_sort | dual ligated metal organic framework as novel multifunctional nanovehicle for targeted drug delivery for hepatic cancer treatment |
| url | https://doi.org/10.1038/s41598-021-99407-5 |
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