Upregulation of Jasmonate-Inducible Defense Proteins and Differential Colonization of Roots of Oryza sativa Cultivars with the Endophyte Azoarcus sp.
The endophyte Azoarcus sp. strain BH72 expresses nitrogenase (nif) genes inside rice roots. We applied a proteomic approach to dissect responses of rice roots toward bacterial colonization and jasmonic acid (JA) treatment. Two sister lineages of Oryza sativa were analyzed with cv. IR42 showing a les...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2006-05-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-0502 |
| _version_ | 1811291406386331648 |
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| author | Lucie Miché Federico Battistoni Sabrina Gemmer Maya Belghazi Barbara Reinhold-Hurek |
| author_facet | Lucie Miché Federico Battistoni Sabrina Gemmer Maya Belghazi Barbara Reinhold-Hurek |
| author_sort | Lucie Miché |
| collection | DOAJ |
| description | The endophyte Azoarcus sp. strain BH72 expresses nitrogenase (nif) genes inside rice roots. We applied a proteomic approach to dissect responses of rice roots toward bacterial colonization and jasmonic acid (JA) treatment. Two sister lineages of Oryza sativa were analyzed with cv. IR42 showing a less compatible interaction with the Azoarcus sp. resulting in slight root browning whereas cv. IR36 was successfully colonized as determined by nifH::gusA activity. External addition of JA inhibited colonization of roots and caused browning in contrast to the addition of ethylene, applied as ethephon (up to 5 mM). Only two of the proteins induced in cv. IR36 by JA were also induced by the endophyte (SalT, two isoforms). In contrast, seven JA-induced proteins were also induced by bacteria in cv. IR42, indicating that IR42 showed a stronger defense response. Mass spectrometry analysis identified these proteins as pathogenesis-related (PR) proteins (Prb1, RSOsPR10) or proteins sharing domains with receptorlike kinases induced by pathogens. Proteins strongly induced in roots in both varieties by JA were identified as Bowman-Birk trypsin inhibittors, germinlike protein, putative endo-1,3-beta-D-glucosidase, glutathion-S-transferase, and 1-propane-1-carboxylate oxidase synthase, peroxidase precursor, PR10-a, and a RAN protein previously not found to be JA-induced. Data suggest that plant defense responses involving JA may contribute to restricting endophytic colonization in grasses. Remarkably, in a compatible interaction with endophytes, JA-inducible stress or defense responses are apparently not important. |
| first_indexed | 2024-04-13T04:28:55Z |
| format | Article |
| id | doaj.art-c0ddcc254c4845b48d4abf6e8de760e9 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:28:55Z |
| publishDate | 2006-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-c0ddcc254c4845b48d4abf6e8de760e92022-12-22T03:02:24ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062006-05-0119550251110.1094/MPMI-19-0502Upregulation of Jasmonate-Inducible Defense Proteins and Differential Colonization of Roots of Oryza sativa Cultivars with the Endophyte Azoarcus sp.Lucie MichéFederico BattistoniSabrina GemmerMaya BelghaziBarbara Reinhold-HurekThe endophyte Azoarcus sp. strain BH72 expresses nitrogenase (nif) genes inside rice roots. We applied a proteomic approach to dissect responses of rice roots toward bacterial colonization and jasmonic acid (JA) treatment. Two sister lineages of Oryza sativa were analyzed with cv. IR42 showing a less compatible interaction with the Azoarcus sp. resulting in slight root browning whereas cv. IR36 was successfully colonized as determined by nifH::gusA activity. External addition of JA inhibited colonization of roots and caused browning in contrast to the addition of ethylene, applied as ethephon (up to 5 mM). Only two of the proteins induced in cv. IR36 by JA were also induced by the endophyte (SalT, two isoforms). In contrast, seven JA-induced proteins were also induced by bacteria in cv. IR42, indicating that IR42 showed a stronger defense response. Mass spectrometry analysis identified these proteins as pathogenesis-related (PR) proteins (Prb1, RSOsPR10) or proteins sharing domains with receptorlike kinases induced by pathogens. Proteins strongly induced in roots in both varieties by JA were identified as Bowman-Birk trypsin inhibittors, germinlike protein, putative endo-1,3-beta-D-glucosidase, glutathion-S-transferase, and 1-propane-1-carboxylate oxidase synthase, peroxidase precursor, PR10-a, and a RAN protein previously not found to be JA-induced. Data suggest that plant defense responses involving JA may contribute to restricting endophytic colonization in grasses. Remarkably, in a compatible interaction with endophytes, JA-inducible stress or defense responses are apparently not important.https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-0502proteomeβ-glucuronidase |
| spellingShingle | Lucie Miché Federico Battistoni Sabrina Gemmer Maya Belghazi Barbara Reinhold-Hurek Upregulation of Jasmonate-Inducible Defense Proteins and Differential Colonization of Roots of Oryza sativa Cultivars with the Endophyte Azoarcus sp. Molecular Plant-Microbe Interactions proteome β-glucuronidase |
| title | Upregulation of Jasmonate-Inducible Defense Proteins and Differential Colonization of Roots of Oryza sativa Cultivars with the Endophyte Azoarcus sp. |
| title_full | Upregulation of Jasmonate-Inducible Defense Proteins and Differential Colonization of Roots of Oryza sativa Cultivars with the Endophyte Azoarcus sp. |
| title_fullStr | Upregulation of Jasmonate-Inducible Defense Proteins and Differential Colonization of Roots of Oryza sativa Cultivars with the Endophyte Azoarcus sp. |
| title_full_unstemmed | Upregulation of Jasmonate-Inducible Defense Proteins and Differential Colonization of Roots of Oryza sativa Cultivars with the Endophyte Azoarcus sp. |
| title_short | Upregulation of Jasmonate-Inducible Defense Proteins and Differential Colonization of Roots of Oryza sativa Cultivars with the Endophyte Azoarcus sp. |
| title_sort | upregulation of jasmonate inducible defense proteins and differential colonization of roots of oryza sativa cultivars with the endophyte azoarcus sp |
| topic | proteome β-glucuronidase |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-0502 |
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