Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application
Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (Striga, Orobanche, and Phelipanche) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 200...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.887347/full |
| _version_ | 1811337792288980992 |
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| author | Alexandre de Saint Germain Guillaume Clavé Paul Schouveiler Jean-Paul Pillot Abhay-Veer Singh Arnaud Chevalier Suzanne Daignan Fornier Ambre Guillory Sandrine Bonhomme Catherine Rameau François-Didier Boyer |
| author_facet | Alexandre de Saint Germain Guillaume Clavé Paul Schouveiler Jean-Paul Pillot Abhay-Veer Singh Arnaud Chevalier Suzanne Daignan Fornier Ambre Guillory Sandrine Bonhomme Catherine Rameau François-Didier Boyer |
| author_sort | Alexandre de Saint Germain |
| collection | DOAJ |
| description | Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (Striga, Orobanche, and Phelipanche) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clavé (GC) probes and performed structure-activity relationship studies on pea, Arabidopsis thaliana, and Physcomitrium (formerly Physcomitrella) patens. The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants. |
| first_indexed | 2024-04-13T18:00:13Z |
| format | Article |
| id | doaj.art-c0de69503cf8493c90992ea69e4d0321 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T18:00:13Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c0de69503cf8493c90992ea69e4d03212022-12-22T02:36:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.887347887347Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right ApplicationAlexandre de Saint Germain0Guillaume Clavé1Paul Schouveiler2Jean-Paul Pillot3Abhay-Veer Singh4Arnaud Chevalier5Suzanne Daignan Fornier6Ambre Guillory7Sandrine Bonhomme8Catherine Rameau9François-Didier Boyer10Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, FranceUniversité Paris-Saclay, CNRS, Institut de Chimie des Substances Naturelles, Gif-sur-Yvette, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, FranceUniversité Paris-Saclay, CNRS, Institut de Chimie des Substances Naturelles, Gif-sur-Yvette, FranceUniversité Paris-Saclay, CNRS, Institut de Chimie des Substances Naturelles, Gif-sur-Yvette, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, FranceUniversité Paris-Saclay, CNRS, Institut de Chimie des Substances Naturelles, Gif-sur-Yvette, FranceStrigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (Striga, Orobanche, and Phelipanche) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clavé (GC) probes and performed structure-activity relationship studies on pea, Arabidopsis thaliana, and Physcomitrium (formerly Physcomitrella) patens. The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants.https://www.frontiersin.org/articles/10.3389/fpls.2022.887347/fullstrigolactoneprofluorescent probespeaArabidopsis thalianaPhyscomitrium patensα/β-hydrolases |
| spellingShingle | Alexandre de Saint Germain Guillaume Clavé Paul Schouveiler Jean-Paul Pillot Abhay-Veer Singh Arnaud Chevalier Suzanne Daignan Fornier Ambre Guillory Sandrine Bonhomme Catherine Rameau François-Didier Boyer Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application Frontiers in Plant Science strigolactone profluorescent probes pea Arabidopsis thaliana Physcomitrium patens α/β-hydrolases |
| title | Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application |
| title_full | Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application |
| title_fullStr | Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application |
| title_full_unstemmed | Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application |
| title_short | Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application |
| title_sort | expansion of the strigolactone profluorescent probes repertory the right probe for the right application |
| topic | strigolactone profluorescent probes pea Arabidopsis thaliana Physcomitrium patens α/β-hydrolases |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.887347/full |
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