Anti-Inflammatory Effect of Liverwort (<i>Marchantia polymorpha</i> L.) and Racomitrium Moss (<i>Racomitrium canescens</i> (Hedw.) Brid.) Growing in Korea
Bryophytes contain a variety of bioactive metabolites, but studies about the anti-inflammatory effect of bryophytes are meager. Therefore, the present study aimed to compare the anti-inflammatory effect of methanol extract of <i>Marchantia polymorpha</i> L. (liverwort) and <i>Racom...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/10/10/2075 |
| _version_ | 1797513368625479680 |
|---|---|
| author | So-Yeon Kim Minji Hong Tae-Hee Kim Ki Yeon Lee Se Jin Park Sun Hee Hong Kandhasamy Sowndhararajan Songmun Kim |
| author_facet | So-Yeon Kim Minji Hong Tae-Hee Kim Ki Yeon Lee Se Jin Park Sun Hee Hong Kandhasamy Sowndhararajan Songmun Kim |
| author_sort | So-Yeon Kim |
| collection | DOAJ |
| description | Bryophytes contain a variety of bioactive metabolites, but studies about the anti-inflammatory effect of bryophytes are meager. Therefore, the present study aimed to compare the anti-inflammatory effect of methanol extract of <i>Marchantia polymorpha</i> L. (liverwort) and <i>Racomitrium canescens</i> (Racomitrium moss) in lipopolysaccharide (LPS)-induced HaCaT cells. To evaluate the anti-inflammatory effect of liverwort and Racomitrium moss, the levels of nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α), and interleukin (IL)-6 and IL-1β in LPS-induced HaCaT cells were measured. The methanol extract of liverwort and Racomitrium moss significantly decreased LPS-induced NO production in HaCaT cells. When compared with Racomitrium moss extract, pre-treatment with methanol extract of liverwort markedly inhibited the expression of iNOS, COX-2, IL-6, and IL-1β at the concentration of 100 µg/mL with the exception of TNF-α. Further, liverwort extract markedly attenuated the production of TNF-α, IL-6, and IL-1β in the culture medium. In addition, ethyl acetate and butanol fractions obtained from the methanol extract of liverwort showed remarkable inhibitory activity against the production of NO in LPS-stimulated HaCaT cells. The LC-MS data revealed the presence of bisbibenzyl types of bioactive components in the methanol extract of liverwort. These data demonstrate that liverwort extract exhibits effective inhibitory activity against the production of inflammatory mediators in LPS-induced HaCaT cells and may be useful for the treatment of inflammation-mediated diseases. |
| first_indexed | 2024-03-10T06:15:36Z |
| format | Article |
| id | doaj.art-c0df471b55a942c9af9263254fc6bbaf |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T06:15:36Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-c0df471b55a942c9af9263254fc6bbaf2023-11-22T19:43:23ZengMDPI AGPlants2223-77472021-09-011010207510.3390/plants10102075Anti-Inflammatory Effect of Liverwort (<i>Marchantia polymorpha</i> L.) and Racomitrium Moss (<i>Racomitrium canescens</i> (Hedw.) Brid.) Growing in KoreaSo-Yeon Kim0Minji Hong1Tae-Hee Kim2Ki Yeon Lee3Se Jin Park4Sun Hee Hong5Kandhasamy Sowndhararajan6Songmun Kim7School of Natural Resource and Environmental Science, Kangwon National University, Chuncheon 24341, KoreaSchool of Natural Resource and Environmental Science, Kangwon National University, Chuncheon 24341, KoreaSchool of Natural Resource and Environmental Science, Kangwon National University, Chuncheon 24341, KoreaSchool of Natural Resource and Environmental Science, Kangwon National University, Chuncheon 24341, KoreaSchool of Natural Resource and Environmental Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Plant Life and Environmental Science, Hankyong National University, Ansung 17579, KoreaDepartment of Botany, Kongunadu Arts and Science College, Coimbatore 641029, IndiaSchool of Natural Resource and Environmental Science, Kangwon National University, Chuncheon 24341, KoreaBryophytes contain a variety of bioactive metabolites, but studies about the anti-inflammatory effect of bryophytes are meager. Therefore, the present study aimed to compare the anti-inflammatory effect of methanol extract of <i>Marchantia polymorpha</i> L. (liverwort) and <i>Racomitrium canescens</i> (Racomitrium moss) in lipopolysaccharide (LPS)-induced HaCaT cells. To evaluate the anti-inflammatory effect of liverwort and Racomitrium moss, the levels of nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α), and interleukin (IL)-6 and IL-1β in LPS-induced HaCaT cells were measured. The methanol extract of liverwort and Racomitrium moss significantly decreased LPS-induced NO production in HaCaT cells. When compared with Racomitrium moss extract, pre-treatment with methanol extract of liverwort markedly inhibited the expression of iNOS, COX-2, IL-6, and IL-1β at the concentration of 100 µg/mL with the exception of TNF-α. Further, liverwort extract markedly attenuated the production of TNF-α, IL-6, and IL-1β in the culture medium. In addition, ethyl acetate and butanol fractions obtained from the methanol extract of liverwort showed remarkable inhibitory activity against the production of NO in LPS-stimulated HaCaT cells. The LC-MS data revealed the presence of bisbibenzyl types of bioactive components in the methanol extract of liverwort. These data demonstrate that liverwort extract exhibits effective inhibitory activity against the production of inflammatory mediators in LPS-induced HaCaT cells and may be useful for the treatment of inflammation-mediated diseases.https://www.mdpi.com/2223-7747/10/10/2075liverwort<i>Marchantia polymorpha</i><i>Racomitrium canescens</i>anti-inflammatorynitric oxideHaCaT cell |
| spellingShingle | So-Yeon Kim Minji Hong Tae-Hee Kim Ki Yeon Lee Se Jin Park Sun Hee Hong Kandhasamy Sowndhararajan Songmun Kim Anti-Inflammatory Effect of Liverwort (<i>Marchantia polymorpha</i> L.) and Racomitrium Moss (<i>Racomitrium canescens</i> (Hedw.) Brid.) Growing in Korea Plants liverwort <i>Marchantia polymorpha</i> <i>Racomitrium canescens</i> anti-inflammatory nitric oxide HaCaT cell |
| title | Anti-Inflammatory Effect of Liverwort (<i>Marchantia polymorpha</i> L.) and Racomitrium Moss (<i>Racomitrium canescens</i> (Hedw.) Brid.) Growing in Korea |
| title_full | Anti-Inflammatory Effect of Liverwort (<i>Marchantia polymorpha</i> L.) and Racomitrium Moss (<i>Racomitrium canescens</i> (Hedw.) Brid.) Growing in Korea |
| title_fullStr | Anti-Inflammatory Effect of Liverwort (<i>Marchantia polymorpha</i> L.) and Racomitrium Moss (<i>Racomitrium canescens</i> (Hedw.) Brid.) Growing in Korea |
| title_full_unstemmed | Anti-Inflammatory Effect of Liverwort (<i>Marchantia polymorpha</i> L.) and Racomitrium Moss (<i>Racomitrium canescens</i> (Hedw.) Brid.) Growing in Korea |
| title_short | Anti-Inflammatory Effect of Liverwort (<i>Marchantia polymorpha</i> L.) and Racomitrium Moss (<i>Racomitrium canescens</i> (Hedw.) Brid.) Growing in Korea |
| title_sort | anti inflammatory effect of liverwort i marchantia polymorpha i l and racomitrium moss i racomitrium canescens i hedw brid growing in korea |
| topic | liverwort <i>Marchantia polymorpha</i> <i>Racomitrium canescens</i> anti-inflammatory nitric oxide HaCaT cell |
| url | https://www.mdpi.com/2223-7747/10/10/2075 |
| work_keys_str_mv | AT soyeonkim antiinflammatoryeffectofliverwortimarchantiapolymorphailandracomitriummossiracomitriumcanescensihedwbridgrowinginkorea AT minjihong antiinflammatoryeffectofliverwortimarchantiapolymorphailandracomitriummossiracomitriumcanescensihedwbridgrowinginkorea AT taeheekim antiinflammatoryeffectofliverwortimarchantiapolymorphailandracomitriummossiracomitriumcanescensihedwbridgrowinginkorea AT kiyeonlee antiinflammatoryeffectofliverwortimarchantiapolymorphailandracomitriummossiracomitriumcanescensihedwbridgrowinginkorea AT sejinpark antiinflammatoryeffectofliverwortimarchantiapolymorphailandracomitriummossiracomitriumcanescensihedwbridgrowinginkorea AT sunheehong antiinflammatoryeffectofliverwortimarchantiapolymorphailandracomitriummossiracomitriumcanescensihedwbridgrowinginkorea AT kandhasamysowndhararajan antiinflammatoryeffectofliverwortimarchantiapolymorphailandracomitriummossiracomitriumcanescensihedwbridgrowinginkorea AT songmunkim antiinflammatoryeffectofliverwortimarchantiapolymorphailandracomitriummossiracomitriumcanescensihedwbridgrowinginkorea |