Standardization of an apical bud in vitro culture protocol for shallot onion (<em>Allium cepa</em> var. <em>aggregatum</em>) regeneration
<p>Shallots (<em>Allium cepa</em> var. <em>aggregatum</em>) are a type of onion which form several bulbs from a single basal disc. They are characterized by a high total solids content which improves their gastronomic and neutraceutical properties. Shallots (<em>A...
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| Format: | Article |
| Language: | English |
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Universidad San Francisco de Quito USFQ
2015-05-01
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| Series: | ACI Avances en Ciencias e Ingenierías |
| Subjects: | |
| Online Access: | http://revistas.usfq.edu.ec/index.php/avances/article/view/225 |
| _version_ | 1797283190960816128 |
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| author | Joely Vega Venancio S. Arahana B. María de Lourdes Torres |
| author_facet | Joely Vega Venancio S. Arahana B. María de Lourdes Torres |
| author_sort | Joely Vega |
| collection | DOAJ |
| description | <p>Shallots (<em>Allium cepa</em> var. <em>aggregatum</em>) are a type of onion which form several bulbs from a single basal disc. They are characterized by a high total solids content which improves their gastronomic and neutraceutical properties. Shallots (<em>Allium cepa</em> var. <em>aggregatum</em>) are a culinary staple with high commercial value in European, North American and South American markets. Their commercial production via vegetative propagation, however, is time-consuming and strongly affected by viral diseases. The aim of this study was to establish an <em>in vitro</em> apical meristem culture protocol for the regeneration of shallot plants. The efficiency of different regeneration and bulb-formation media were evaluated in this investigation. The best results for plant regeneration (68 % efficiency) were obtained with MS + 30g. L<sup>-1</sup> sucrose + 1.5g. L<sup>-1</sup> benomyl + 1.1uM NAA + 8.9uM BAP and for bulb formation (77% efficiency) with MS + 10uM ancymidol and 50g. L<sup>-1</sup> sucrose. Moreover, 50% of all plants regenerated from apical meristems were effectively acclimatized. The protocol developed and standardized in this investigation can be used to increase the efficiency of shallot bulb-seed production for commercial applications.</p> |
| first_indexed | 2024-03-07T17:26:35Z |
| format | Article |
| id | doaj.art-c0dfdf13bf5c437b87d0827e9002e25d |
| institution | Directory Open Access Journal |
| issn | 1390-5384 2528-7788 |
| language | English |
| last_indexed | 2024-03-07T17:26:35Z |
| publishDate | 2015-05-01 |
| publisher | Universidad San Francisco de Quito USFQ |
| record_format | Article |
| series | ACI Avances en Ciencias e Ingenierías |
| spelling | doaj.art-c0dfdf13bf5c437b87d0827e9002e25d2024-03-02T18:55:32ZengUniversidad San Francisco de Quito USFQACI Avances en Ciencias e Ingenierías1390-53842528-77882015-05-017110.18272/aci.v7i1.225224Standardization of an apical bud in vitro culture protocol for shallot onion (<em>Allium cepa</em> var. <em>aggregatum</em>) regenerationJoely Vega0Venancio S. Arahana B.1María de Lourdes Torres2Universidad San Francisco de QuitoUniversidad San Francisco de QuitoUniversidad San Francisco de Quito<p>Shallots (<em>Allium cepa</em> var. <em>aggregatum</em>) are a type of onion which form several bulbs from a single basal disc. They are characterized by a high total solids content which improves their gastronomic and neutraceutical properties. Shallots (<em>Allium cepa</em> var. <em>aggregatum</em>) are a culinary staple with high commercial value in European, North American and South American markets. Their commercial production via vegetative propagation, however, is time-consuming and strongly affected by viral diseases. The aim of this study was to establish an <em>in vitro</em> apical meristem culture protocol for the regeneration of shallot plants. The efficiency of different regeneration and bulb-formation media were evaluated in this investigation. The best results for plant regeneration (68 % efficiency) were obtained with MS + 30g. L<sup>-1</sup> sucrose + 1.5g. L<sup>-1</sup> benomyl + 1.1uM NAA + 8.9uM BAP and for bulb formation (77% efficiency) with MS + 10uM ancymidol and 50g. L<sup>-1</sup> sucrose. Moreover, 50% of all plants regenerated from apical meristems were effectively acclimatized. The protocol developed and standardized in this investigation can be used to increase the efficiency of shallot bulb-seed production for commercial applications.</p>http://revistas.usfq.edu.ec/index.php/avances/article/view/225Allium cepa var. aggregatummeristema apicalcultivo in vitro |
| spellingShingle | Joely Vega Venancio S. Arahana B. María de Lourdes Torres Standardization of an apical bud in vitro culture protocol for shallot onion (<em>Allium cepa</em> var. <em>aggregatum</em>) regeneration ACI Avances en Ciencias e Ingenierías Allium cepa var. aggregatum meristema apical cultivo in vitro |
| title | Standardization of an apical bud in vitro culture protocol for shallot onion (<em>Allium cepa</em> var. <em>aggregatum</em>) regeneration |
| title_full | Standardization of an apical bud in vitro culture protocol for shallot onion (<em>Allium cepa</em> var. <em>aggregatum</em>) regeneration |
| title_fullStr | Standardization of an apical bud in vitro culture protocol for shallot onion (<em>Allium cepa</em> var. <em>aggregatum</em>) regeneration |
| title_full_unstemmed | Standardization of an apical bud in vitro culture protocol for shallot onion (<em>Allium cepa</em> var. <em>aggregatum</em>) regeneration |
| title_short | Standardization of an apical bud in vitro culture protocol for shallot onion (<em>Allium cepa</em> var. <em>aggregatum</em>) regeneration |
| title_sort | standardization of an apical bud in vitro culture protocol for shallot onion em allium cepa em var em aggregatum em regeneration |
| topic | Allium cepa var. aggregatum meristema apical cultivo in vitro |
| url | http://revistas.usfq.edu.ec/index.php/avances/article/view/225 |
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