Species and sex-specific chemosensory gene expression in Anopheles coluzzii and An. quadriannulatus antennae
Abstract Background Olfactory cues drive mosquito behaviors such as host-seeking, locating sugar sources and oviposition. These behaviors can vary between sexes and closely related species. For example, the malaria vector Anopheles coluzzii is highly anthropophilic, whereas An. quadriannulatus is no...
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-020-04085-3 |
| _version_ | 1818409188904140800 |
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| author | Giridhar Athrey Zachary Popkin-Hall Luciano Veiga Cosme Willem Takken Michel Andre Slotman |
| author_facet | Giridhar Athrey Zachary Popkin-Hall Luciano Veiga Cosme Willem Takken Michel Andre Slotman |
| author_sort | Giridhar Athrey |
| collection | DOAJ |
| description | Abstract Background Olfactory cues drive mosquito behaviors such as host-seeking, locating sugar sources and oviposition. These behaviors can vary between sexes and closely related species. For example, the malaria vector Anopheles coluzzii is highly anthropophilic, whereas An. quadriannulatus is not. These behavioral differences may be reflected in chemosensory gene expression. Methods The expression of chemosensory genes in the antennae of both sexes of An. coluzzii and An. quadriannulatus was compared using RNA-seq. The sex-biased expression of several genes in An. coluzzii was also compared using qPCR. Results The chemosensory expression is mostly similar in the male antennae of An. coluzzii and An. quadriannulatus, with only a few modest differences in expression. A handful of chemosensory genes are male-biased in both species; the highly expressed gustatory receptor AgGr33, odorant binding proteins AgObp25, AgObp26 and possibly AgObp10. Although the chemosensory gene repertoire is mostly shared between the sexes, several highly female-biased AgOrs, AgIrs, and one AgObp were identified, including several whose expression is biased towards the anthropophilic An. coluzzii. Additionally, the expression of several chemosensory genes is biased towards An. coluzzii in both sexes. Conclusions Chemosensory gene expression is broadly similar between species and sexes, but several sex- biased/specific genes were identified. These may modulate sex- and species-specific behaviors. Although the male behavior of these species remains poorly studied, the identification of sex- and species-specific chemosensory genes may provide fertile ground for future work. |
| first_indexed | 2024-12-14T09:55:40Z |
| format | Article |
| id | doaj.art-c0e0893c120d418bba7bd19ea831440d |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-14T09:55:40Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-c0e0893c120d418bba7bd19ea831440d2022-12-21T23:07:24ZengBMCParasites & Vectors1756-33052020-04-0113111510.1186/s13071-020-04085-3Species and sex-specific chemosensory gene expression in Anopheles coluzzii and An. quadriannulatus antennaeGiridhar Athrey0Zachary Popkin-Hall1Luciano Veiga Cosme2Willem Takken3Michel Andre Slotman4Department of Poultry Science, Texas A&M UniversityDepartment of Entomology, Texas A&M UniversityDepartment of Ecology and Evolutionary Biology, Yale UniversityLaboratory of Entomology, Wageningen University and ResearchDepartment of Entomology, Texas A&M UniversityAbstract Background Olfactory cues drive mosquito behaviors such as host-seeking, locating sugar sources and oviposition. These behaviors can vary between sexes and closely related species. For example, the malaria vector Anopheles coluzzii is highly anthropophilic, whereas An. quadriannulatus is not. These behavioral differences may be reflected in chemosensory gene expression. Methods The expression of chemosensory genes in the antennae of both sexes of An. coluzzii and An. quadriannulatus was compared using RNA-seq. The sex-biased expression of several genes in An. coluzzii was also compared using qPCR. Results The chemosensory expression is mostly similar in the male antennae of An. coluzzii and An. quadriannulatus, with only a few modest differences in expression. A handful of chemosensory genes are male-biased in both species; the highly expressed gustatory receptor AgGr33, odorant binding proteins AgObp25, AgObp26 and possibly AgObp10. Although the chemosensory gene repertoire is mostly shared between the sexes, several highly female-biased AgOrs, AgIrs, and one AgObp were identified, including several whose expression is biased towards the anthropophilic An. coluzzii. Additionally, the expression of several chemosensory genes is biased towards An. coluzzii in both sexes. Conclusions Chemosensory gene expression is broadly similar between species and sexes, but several sex- biased/specific genes were identified. These may modulate sex- and species-specific behaviors. Although the male behavior of these species remains poorly studied, the identification of sex- and species-specific chemosensory genes may provide fertile ground for future work.http://link.springer.com/article/10.1186/s13071-020-04085-3AnophelesChemosensationOlfactionHost seekingMating |
| spellingShingle | Giridhar Athrey Zachary Popkin-Hall Luciano Veiga Cosme Willem Takken Michel Andre Slotman Species and sex-specific chemosensory gene expression in Anopheles coluzzii and An. quadriannulatus antennae Parasites & Vectors Anopheles Chemosensation Olfaction Host seeking Mating |
| title | Species and sex-specific chemosensory gene expression in Anopheles coluzzii and An. quadriannulatus antennae |
| title_full | Species and sex-specific chemosensory gene expression in Anopheles coluzzii and An. quadriannulatus antennae |
| title_fullStr | Species and sex-specific chemosensory gene expression in Anopheles coluzzii and An. quadriannulatus antennae |
| title_full_unstemmed | Species and sex-specific chemosensory gene expression in Anopheles coluzzii and An. quadriannulatus antennae |
| title_short | Species and sex-specific chemosensory gene expression in Anopheles coluzzii and An. quadriannulatus antennae |
| title_sort | species and sex specific chemosensory gene expression in anopheles coluzzii and an quadriannulatus antennae |
| topic | Anopheles Chemosensation Olfaction Host seeking Mating |
| url | http://link.springer.com/article/10.1186/s13071-020-04085-3 |
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