HrpS Is a Global Regulator on Type III Secretion System (T3SS) and Non-T3SS Genes in Pseudomonas savastanoi pv. phaseolicola
The type III secretion system (T3SS) is the main machinery for Pseudomonas savastanoi and other gram-negative bacteria to invade plant cells. HrpR and HrpS form a hetero-hexamer, which activates the expression of HrpL, which induces all T3SS genes by binding to a ‘hrp box’ in promoters. However, the...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2018-12-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-02-18-0035-R |
| _version_ | 1819045992939388928 |
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| author | Jingru Wang Xiaolong Shao Yingchao Zhang Yanan Zhu Pan Yang Jian Yuan Tingting Wang Chunyan Yin Wei Wang Sheng Chen Haihua Liang Xin Deng |
| author_facet | Jingru Wang Xiaolong Shao Yingchao Zhang Yanan Zhu Pan Yang Jian Yuan Tingting Wang Chunyan Yin Wei Wang Sheng Chen Haihua Liang Xin Deng |
| author_sort | Jingru Wang |
| collection | DOAJ |
| description | The type III secretion system (T3SS) is the main machinery for Pseudomonas savastanoi and other gram-negative bacteria to invade plant cells. HrpR and HrpS form a hetero-hexamer, which activates the expression of HrpL, which induces all T3SS genes by binding to a ‘hrp box’ in promoters. However, the individual molecular mechanism of HrpR or HrpS has not been fully understood. Through chromatin immunoprecipitation coupled to high-throughput DNA sequencing, we found that HrpR, HrpS, and HrpL had four, 47, and 31 targets on the genome, respectively. HrpS directly bound to the promoter regions of a group of T3SS genes and non-T3SS genes. HrpS independently regulated these genes in a hrpL deletion strain. Additionally, a HrpS-binding motif (GTGCCAAA) was identified, which was verified by electrophoretic mobility shift assay and lux-reporter assay. HrpS also regulated motility and biofilm formation in P. savastanoi. The present study strongly suggests that HrpS alone can work as a global regulator on both T3SS and non-T3SS genes in P. savastanoi.[Graphic: see text] Copyright © 2018 The Author(s). This is an open-access article distributed under the CC BY-NC-ND 4.0 International license. |
| first_indexed | 2024-12-21T10:37:23Z |
| format | Article |
| id | doaj.art-c0e118e48ed94276ba8d4b67dbaaa8e3 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-21T10:37:23Z |
| publishDate | 2018-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-c0e118e48ed94276ba8d4b67dbaaa8e32022-12-21T19:07:03ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062018-12-0131121232124310.1094/MPMI-02-18-0035-RHrpS Is a Global Regulator on Type III Secretion System (T3SS) and Non-T3SS Genes in Pseudomonas savastanoi pv. phaseolicolaJingru Wang0Xiaolong Shao1Yingchao Zhang2Yanan Zhu3Pan Yang4Jian Yuan5Tingting Wang6Chunyan Yin7Wei Wang8Sheng Chen9Haihua Liang10Xin Deng11Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin, 300457, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin, 300457, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin, 300457, China;Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, U.S.A.;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin, 300457, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin, 300457, China;Department of Biomedical Sciences, City University of Hong Kong, 83 Tat Chee Rd, Kowloon Tong, Hong Kong;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin, 300457, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin, 300457, China;Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong; andKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi’an, ShaanXi 710069, ChinaDepartment of Biomedical Sciences, City University of Hong Kong, 83 Tat Chee Rd, Kowloon Tong, Hong Kong;The type III secretion system (T3SS) is the main machinery for Pseudomonas savastanoi and other gram-negative bacteria to invade plant cells. HrpR and HrpS form a hetero-hexamer, which activates the expression of HrpL, which induces all T3SS genes by binding to a ‘hrp box’ in promoters. However, the individual molecular mechanism of HrpR or HrpS has not been fully understood. Through chromatin immunoprecipitation coupled to high-throughput DNA sequencing, we found that HrpR, HrpS, and HrpL had four, 47, and 31 targets on the genome, respectively. HrpS directly bound to the promoter regions of a group of T3SS genes and non-T3SS genes. HrpS independently regulated these genes in a hrpL deletion strain. Additionally, a HrpS-binding motif (GTGCCAAA) was identified, which was verified by electrophoretic mobility shift assay and lux-reporter assay. HrpS also regulated motility and biofilm formation in P. savastanoi. The present study strongly suggests that HrpS alone can work as a global regulator on both T3SS and non-T3SS genes in P. savastanoi.[Graphic: see text] Copyright © 2018 The Author(s). This is an open-access article distributed under the CC BY-NC-ND 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/MPMI-02-18-0035-R |
| spellingShingle | Jingru Wang Xiaolong Shao Yingchao Zhang Yanan Zhu Pan Yang Jian Yuan Tingting Wang Chunyan Yin Wei Wang Sheng Chen Haihua Liang Xin Deng HrpS Is a Global Regulator on Type III Secretion System (T3SS) and Non-T3SS Genes in Pseudomonas savastanoi pv. phaseolicola Molecular Plant-Microbe Interactions |
| title | HrpS Is a Global Regulator on Type III Secretion System (T3SS) and Non-T3SS Genes in Pseudomonas savastanoi pv. phaseolicola |
| title_full | HrpS Is a Global Regulator on Type III Secretion System (T3SS) and Non-T3SS Genes in Pseudomonas savastanoi pv. phaseolicola |
| title_fullStr | HrpS Is a Global Regulator on Type III Secretion System (T3SS) and Non-T3SS Genes in Pseudomonas savastanoi pv. phaseolicola |
| title_full_unstemmed | HrpS Is a Global Regulator on Type III Secretion System (T3SS) and Non-T3SS Genes in Pseudomonas savastanoi pv. phaseolicola |
| title_short | HrpS Is a Global Regulator on Type III Secretion System (T3SS) and Non-T3SS Genes in Pseudomonas savastanoi pv. phaseolicola |
| title_sort | hrps is a global regulator on type iii secretion system t3ss and non t3ss genes in pseudomonas savastanoi pv phaseolicola |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-02-18-0035-R |
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