TBX2 affects proliferation, apoptosis and cholesterol generation by regulating mitochondrial function and autophagy in bovine cumulus cell
Abstract Background T‐box transcription factor 2 (TBX2) is a member of T‐box gene family whose members are highly conserved in evolution and encoding genes and are involved in the regulation of developmental processes. The encoding genes play an important role in growth and development. Although TBX...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.1009 |
| _version_ | 1797946536073625600 |
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| author | Sheng‐Peng Li Hao Jiang Zi‐Bin Liu Wen‐Jie Yu Xiao‐Shi Cai Chang Liu Wen‐Yin Xie Fu‐shi Quan Wei Gao Nam‐Hyung Kim Bao Yuan Cheng‐Zhen Chen Jia‐Bao Zhang |
| author_facet | Sheng‐Peng Li Hao Jiang Zi‐Bin Liu Wen‐Jie Yu Xiao‐Shi Cai Chang Liu Wen‐Yin Xie Fu‐shi Quan Wei Gao Nam‐Hyung Kim Bao Yuan Cheng‐Zhen Chen Jia‐Bao Zhang |
| author_sort | Sheng‐Peng Li |
| collection | DOAJ |
| description | Abstract Background T‐box transcription factor 2 (TBX2) is a member of T‐box gene family whose members are highly conserved in evolution and encoding genes and are involved in the regulation of developmental processes. The encoding genes play an important role in growth and development. Although TBX2 has been widely studied in cancer cell growth and development, its biological functions in bovine cumulus cells remain unclear. Objectives This study aimed to investigate the regulatory effects of TBX2 in bovine cumulus cells. Methods TBX2 gene was knockdown with siRNA to clarify the function in cellular physiological processes. Cell proliferation and cycle changes were determined by xCELLigence cell function analyzer and flow cytometry. Mitochondrial membrane potential and autophagy were detected by fluorescent dye staining and immunofluorescence techniques. Western blot and quantitative real‐time reverse transcription polymerase chain reaction (qRT‐PCR) were used to detect the expression changes of proliferation and autophagy‐related proteins. Aadenosine triphosphate (ATP) production, glucose metabolism, and cholesterol synthesis of cumulus cells were measured by optical density and chemiluminescence analysis. Results After inhibition of TBX2, the cell cycle was disrupted. The levels of apoptosis, ratio of light chain 3 beta II/I, and reactive oxygen species were increased. The proliferation, expansion ability, ATP production, and the amount of cholesterol secreted by cumulus cells were significantly decreased. Conclusions TBX2 plays important roles in regulating the cells’ proliferation, expansion, apoptosis, and autophagy; maintaining the mitochondrial function and cholesterol generation of bovine cumulus cells. |
| first_indexed | 2024-04-10T21:13:29Z |
| format | Article |
| id | doaj.art-c0e3aa36fa244519a4238b8a485f76b9 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-04-10T21:13:29Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-c0e3aa36fa244519a4238b8a485f76b92023-01-20T17:53:08ZengWileyVeterinary Medicine and Science2053-10952023-01-019132633510.1002/vms3.1009TBX2 affects proliferation, apoptosis and cholesterol generation by regulating mitochondrial function and autophagy in bovine cumulus cellSheng‐Peng Li0Hao Jiang1Zi‐Bin Liu2Wen‐Jie Yu3Xiao‐Shi Cai4Chang Liu5Wen‐Yin Xie6Fu‐shi Quan7Wei Gao8Nam‐Hyung Kim9Bao Yuan10Cheng‐Zhen Chen11Jia‐Bao Zhang12Department of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaDepartment of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaDepartment of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaDepartment of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaDepartment of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaSchool of Grains Jilin Business and Technology College Changchun Jilin ChinaDepartment of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaDepartment of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaDepartment of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaDepartment of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaDepartment of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaDepartment of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaDepartment of Laboratory Animals Jilin Provincial Key Laboratory of Animal Model Jilin University Changchun Jilin ChinaAbstract Background T‐box transcription factor 2 (TBX2) is a member of T‐box gene family whose members are highly conserved in evolution and encoding genes and are involved in the regulation of developmental processes. The encoding genes play an important role in growth and development. Although TBX2 has been widely studied in cancer cell growth and development, its biological functions in bovine cumulus cells remain unclear. Objectives This study aimed to investigate the regulatory effects of TBX2 in bovine cumulus cells. Methods TBX2 gene was knockdown with siRNA to clarify the function in cellular physiological processes. Cell proliferation and cycle changes were determined by xCELLigence cell function analyzer and flow cytometry. Mitochondrial membrane potential and autophagy were detected by fluorescent dye staining and immunofluorescence techniques. Western blot and quantitative real‐time reverse transcription polymerase chain reaction (qRT‐PCR) were used to detect the expression changes of proliferation and autophagy‐related proteins. Aadenosine triphosphate (ATP) production, glucose metabolism, and cholesterol synthesis of cumulus cells were measured by optical density and chemiluminescence analysis. Results After inhibition of TBX2, the cell cycle was disrupted. The levels of apoptosis, ratio of light chain 3 beta II/I, and reactive oxygen species were increased. The proliferation, expansion ability, ATP production, and the amount of cholesterol secreted by cumulus cells were significantly decreased. Conclusions TBX2 plays important roles in regulating the cells’ proliferation, expansion, apoptosis, and autophagy; maintaining the mitochondrial function and cholesterol generation of bovine cumulus cells.https://doi.org/10.1002/vms3.1009autophagycumulus cellsphysiological functionreactive oxygen speciesT‐box transcription factor 2 |
| spellingShingle | Sheng‐Peng Li Hao Jiang Zi‐Bin Liu Wen‐Jie Yu Xiao‐Shi Cai Chang Liu Wen‐Yin Xie Fu‐shi Quan Wei Gao Nam‐Hyung Kim Bao Yuan Cheng‐Zhen Chen Jia‐Bao Zhang TBX2 affects proliferation, apoptosis and cholesterol generation by regulating mitochondrial function and autophagy in bovine cumulus cell Veterinary Medicine and Science autophagy cumulus cells physiological function reactive oxygen species T‐box transcription factor 2 |
| title | TBX2 affects proliferation, apoptosis and cholesterol generation by regulating mitochondrial function and autophagy in bovine cumulus cell |
| title_full | TBX2 affects proliferation, apoptosis and cholesterol generation by regulating mitochondrial function and autophagy in bovine cumulus cell |
| title_fullStr | TBX2 affects proliferation, apoptosis and cholesterol generation by regulating mitochondrial function and autophagy in bovine cumulus cell |
| title_full_unstemmed | TBX2 affects proliferation, apoptosis and cholesterol generation by regulating mitochondrial function and autophagy in bovine cumulus cell |
| title_short | TBX2 affects proliferation, apoptosis and cholesterol generation by regulating mitochondrial function and autophagy in bovine cumulus cell |
| title_sort | tbx2 affects proliferation apoptosis and cholesterol generation by regulating mitochondrial function and autophagy in bovine cumulus cell |
| topic | autophagy cumulus cells physiological function reactive oxygen species T‐box transcription factor 2 |
| url | https://doi.org/10.1002/vms3.1009 |
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