Assessment of Polyphenols Bioaccessibility, Stability, and Antioxidant Activity of White Mugwort (<i>Artemisia lactiflora</i> Wall.) during Static In Vitro Gastrointestinal Digestion
White mugwort (<i>Artemisia lactiflora</i> Wall.), a traditional Chinese medicine, has been widely consumed in different forms for health care purposes. In this study, the in vitro digestion model of INFOGEST was used to investigate the bioaccessibility, stability, and antioxidant activi...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/12/5/949 |
| _version_ | 1797615407053406208 |
|---|---|
| author | Nacha Udomwasinakun Shikha Saha Ana-Isabel Mulet-Cabero Peter James Wilde Tantawan Pirak |
| author_facet | Nacha Udomwasinakun Shikha Saha Ana-Isabel Mulet-Cabero Peter James Wilde Tantawan Pirak |
| author_sort | Nacha Udomwasinakun |
| collection | DOAJ |
| description | White mugwort (<i>Artemisia lactiflora</i> Wall.), a traditional Chinese medicine, has been widely consumed in different forms for health care purposes. In this study, the in vitro digestion model of INFOGEST was used to investigate the bioaccessibility, stability, and antioxidant activity of polyphenols from two different forms of white mugwort, including dried powder (P 50, 100, and 150 mg/mL) and fresh extract (FE 5, 15, and 30 mg/mL). During digestion, the bioaccessibility of TPC and antioxidant activity were influenced by the form and ingested concentration of white mugwort. The highest bioaccessibility of the total phenolic content (TPC) and relative antioxidant activity were found at the lowest P and FE concentrations, as calculated relative to the TPC and antioxidant activity of P-MetOH and FE-MetOH based on the dry weight of the sample. Post-digestion, in comparison to P, FE had higher bioaccessibility (FE = 287.7% and P = 130.7%), relative DPPH radical scavenging activity (FE = 104.2% and P = 47.3%), and relative FRAP (FE = 673.5% and P = 66.5%). Nine compounds, 3-caffeoylquinic acid, 5-caffeoylquinic acid, 3,5-di-caffeoylquinic acid, sinapolymalate, isovitexin, kaempferol, morin, rutin, and quercetin, identified in both samples were modified during digestion, yet still provided strong antioxidant activity. These findings suggest that white mugwort extract possesses a higher polyphenol bioaccessibility, showing great potential as a functional ingredient. |
| first_indexed | 2024-03-11T07:25:50Z |
| format | Article |
| id | doaj.art-c0e50a22b3d44292bfba2aa3d8632faf |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T07:25:50Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-c0e50a22b3d44292bfba2aa3d8632faf2023-11-17T07:40:11ZengMDPI AGFoods2304-81582023-02-0112594910.3390/foods12050949Assessment of Polyphenols Bioaccessibility, Stability, and Antioxidant Activity of White Mugwort (<i>Artemisia lactiflora</i> Wall.) during Static In Vitro Gastrointestinal DigestionNacha Udomwasinakun0Shikha Saha1Ana-Isabel Mulet-Cabero2Peter James Wilde3Tantawan Pirak4Department of Product Development, Faculty of Agro Industry, Kasetsart University, 50 Ngamwomgwan, Lat Yao, Chatuchak, Bangkok 10900, ThailandQuadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UKQuadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UKQuadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UKDepartment of Product Development, Faculty of Agro Industry, Kasetsart University, 50 Ngamwomgwan, Lat Yao, Chatuchak, Bangkok 10900, ThailandWhite mugwort (<i>Artemisia lactiflora</i> Wall.), a traditional Chinese medicine, has been widely consumed in different forms for health care purposes. In this study, the in vitro digestion model of INFOGEST was used to investigate the bioaccessibility, stability, and antioxidant activity of polyphenols from two different forms of white mugwort, including dried powder (P 50, 100, and 150 mg/mL) and fresh extract (FE 5, 15, and 30 mg/mL). During digestion, the bioaccessibility of TPC and antioxidant activity were influenced by the form and ingested concentration of white mugwort. The highest bioaccessibility of the total phenolic content (TPC) and relative antioxidant activity were found at the lowest P and FE concentrations, as calculated relative to the TPC and antioxidant activity of P-MetOH and FE-MetOH based on the dry weight of the sample. Post-digestion, in comparison to P, FE had higher bioaccessibility (FE = 287.7% and P = 130.7%), relative DPPH radical scavenging activity (FE = 104.2% and P = 47.3%), and relative FRAP (FE = 673.5% and P = 66.5%). Nine compounds, 3-caffeoylquinic acid, 5-caffeoylquinic acid, 3,5-di-caffeoylquinic acid, sinapolymalate, isovitexin, kaempferol, morin, rutin, and quercetin, identified in both samples were modified during digestion, yet still provided strong antioxidant activity. These findings suggest that white mugwort extract possesses a higher polyphenol bioaccessibility, showing great potential as a functional ingredient.https://www.mdpi.com/2304-8158/12/5/949white mugwort<i>Artemisia lactiflora</i> Wall.in vitro digestionantioxidantpolyphenol profilebioaccessibility |
| spellingShingle | Nacha Udomwasinakun Shikha Saha Ana-Isabel Mulet-Cabero Peter James Wilde Tantawan Pirak Assessment of Polyphenols Bioaccessibility, Stability, and Antioxidant Activity of White Mugwort (<i>Artemisia lactiflora</i> Wall.) during Static In Vitro Gastrointestinal Digestion Foods white mugwort <i>Artemisia lactiflora</i> Wall. in vitro digestion antioxidant polyphenol profile bioaccessibility |
| title | Assessment of Polyphenols Bioaccessibility, Stability, and Antioxidant Activity of White Mugwort (<i>Artemisia lactiflora</i> Wall.) during Static In Vitro Gastrointestinal Digestion |
| title_full | Assessment of Polyphenols Bioaccessibility, Stability, and Antioxidant Activity of White Mugwort (<i>Artemisia lactiflora</i> Wall.) during Static In Vitro Gastrointestinal Digestion |
| title_fullStr | Assessment of Polyphenols Bioaccessibility, Stability, and Antioxidant Activity of White Mugwort (<i>Artemisia lactiflora</i> Wall.) during Static In Vitro Gastrointestinal Digestion |
| title_full_unstemmed | Assessment of Polyphenols Bioaccessibility, Stability, and Antioxidant Activity of White Mugwort (<i>Artemisia lactiflora</i> Wall.) during Static In Vitro Gastrointestinal Digestion |
| title_short | Assessment of Polyphenols Bioaccessibility, Stability, and Antioxidant Activity of White Mugwort (<i>Artemisia lactiflora</i> Wall.) during Static In Vitro Gastrointestinal Digestion |
| title_sort | assessment of polyphenols bioaccessibility stability and antioxidant activity of white mugwort i artemisia lactiflora i wall during static in vitro gastrointestinal digestion |
| topic | white mugwort <i>Artemisia lactiflora</i> Wall. in vitro digestion antioxidant polyphenol profile bioaccessibility |
| url | https://www.mdpi.com/2304-8158/12/5/949 |
| work_keys_str_mv | AT nachaudomwasinakun assessmentofpolyphenolsbioaccessibilitystabilityandantioxidantactivityofwhitemugwortiartemisialactifloraiwallduringstaticinvitrogastrointestinaldigestion AT shikhasaha assessmentofpolyphenolsbioaccessibilitystabilityandantioxidantactivityofwhitemugwortiartemisialactifloraiwallduringstaticinvitrogastrointestinaldigestion AT anaisabelmuletcabero assessmentofpolyphenolsbioaccessibilitystabilityandantioxidantactivityofwhitemugwortiartemisialactifloraiwallduringstaticinvitrogastrointestinaldigestion AT peterjameswilde assessmentofpolyphenolsbioaccessibilitystabilityandantioxidantactivityofwhitemugwortiartemisialactifloraiwallduringstaticinvitrogastrointestinaldigestion AT tantawanpirak assessmentofpolyphenolsbioaccessibilitystabilityandantioxidantactivityofwhitemugwortiartemisialactifloraiwallduringstaticinvitrogastrointestinaldigestion |