Proteomic Identification of Plasma Components in <i>Tachypleus tridentatus</i> and Their Effects on the Longitudinal Bone Growth Rate in Rats
<i>Tachypleus tridentatus</i> (<i>T. tridentatus</i>) is a marine animal and traditional Chinese medicine. <i>T. tridentatus</i> plasma is a valuable resource for important medical and health-based functions. In this experiment, in order to evaluate the effect and...
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MDPI AG
2023-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/2/111 |
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| author | Shu Jiang Xinjian Qu Siping Liu Jun Wei Xiangxi Yi Yonghong Liu Chenghai Gao |
| author_facet | Shu Jiang Xinjian Qu Siping Liu Jun Wei Xiangxi Yi Yonghong Liu Chenghai Gao |
| author_sort | Shu Jiang |
| collection | DOAJ |
| description | <i>Tachypleus tridentatus</i> (<i>T. tridentatus</i>) is a marine animal and traditional Chinese medicine. <i>T. tridentatus</i> plasma is a valuable resource for important medical and health-based functions. In this experiment, in order to evaluate the effect and mechanism of <i>T. tridentatus</i> plasma with respect to the promotion of bone tissue growth in rats, the processes of ultrafiltration and mass spectrometry were first used to separate and identify the components of <i>T. tridentatus</i> plasma. Then, a comparison of the effects of the <i>T. tridentatus</i> plasma samples, which each possessed different molecular weights, regarding the growth of the long bones of rats was conducted. Finally, transcriptomics, proteomics, and bioinformatics were all used to analyze the biological functions and related signaling pathways of the <i>T. tridentatus</i> plasma in order to promote rat bone growth. The results showed that the contents of amino acid residues in peptides are related to the growth promotion that was contained in the 10–30 kDa plasma group. Moreover, the <i>T. tridentatus</i> plasma samples were found to be higher in this respect than those in the whole plasma group. In addition, the 10–30 kDa plasma group could significantly promote bone growth activity in rats. The proteomic analysis showed that the proteins that were differentially expressed in the 10–30 kDa plasma group were mainly enriched in the PI3K-AKT signal pathway. Our study suggested that the <i>T. tridentatus</i> plasma possesses promising potential for the purposes of clinical use, whereby it can serve the role of a growth-promoting agent. |
| first_indexed | 2024-03-11T08:31:50Z |
| format | Article |
| id | doaj.art-c0e77704e7ad403985522942272b8213 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T08:31:50Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-c0e77704e7ad403985522942272b82132023-11-16T21:47:57ZengMDPI AGMarine Drugs1660-33972023-02-0121211110.3390/md21020111Proteomic Identification of Plasma Components in <i>Tachypleus tridentatus</i> and Their Effects on the Longitudinal Bone Growth Rate in RatsShu Jiang0Xinjian Qu1Siping Liu2Jun Wei3Xiangxi Yi4Yonghong Liu5Chenghai Gao6Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, ChinaInstitute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, ChinaInstitute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, ChinaInstitute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, ChinaInstitute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, ChinaInstitute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, ChinaInstitute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China<i>Tachypleus tridentatus</i> (<i>T. tridentatus</i>) is a marine animal and traditional Chinese medicine. <i>T. tridentatus</i> plasma is a valuable resource for important medical and health-based functions. In this experiment, in order to evaluate the effect and mechanism of <i>T. tridentatus</i> plasma with respect to the promotion of bone tissue growth in rats, the processes of ultrafiltration and mass spectrometry were first used to separate and identify the components of <i>T. tridentatus</i> plasma. Then, a comparison of the effects of the <i>T. tridentatus</i> plasma samples, which each possessed different molecular weights, regarding the growth of the long bones of rats was conducted. Finally, transcriptomics, proteomics, and bioinformatics were all used to analyze the biological functions and related signaling pathways of the <i>T. tridentatus</i> plasma in order to promote rat bone growth. The results showed that the contents of amino acid residues in peptides are related to the growth promotion that was contained in the 10–30 kDa plasma group. Moreover, the <i>T. tridentatus</i> plasma samples were found to be higher in this respect than those in the whole plasma group. In addition, the 10–30 kDa plasma group could significantly promote bone growth activity in rats. The proteomic analysis showed that the proteins that were differentially expressed in the 10–30 kDa plasma group were mainly enriched in the PI3K-AKT signal pathway. Our study suggested that the <i>T. tridentatus</i> plasma possesses promising potential for the purposes of clinical use, whereby it can serve the role of a growth-promoting agent.https://www.mdpi.com/1660-3397/21/2/111<i>Tachypleus tridentatus</i>longitudinal bone growthtranscriptomicsproteomics |
| spellingShingle | Shu Jiang Xinjian Qu Siping Liu Jun Wei Xiangxi Yi Yonghong Liu Chenghai Gao Proteomic Identification of Plasma Components in <i>Tachypleus tridentatus</i> and Their Effects on the Longitudinal Bone Growth Rate in Rats Marine Drugs <i>Tachypleus tridentatus</i> longitudinal bone growth transcriptomics proteomics |
| title | Proteomic Identification of Plasma Components in <i>Tachypleus tridentatus</i> and Their Effects on the Longitudinal Bone Growth Rate in Rats |
| title_full | Proteomic Identification of Plasma Components in <i>Tachypleus tridentatus</i> and Their Effects on the Longitudinal Bone Growth Rate in Rats |
| title_fullStr | Proteomic Identification of Plasma Components in <i>Tachypleus tridentatus</i> and Their Effects on the Longitudinal Bone Growth Rate in Rats |
| title_full_unstemmed | Proteomic Identification of Plasma Components in <i>Tachypleus tridentatus</i> and Their Effects on the Longitudinal Bone Growth Rate in Rats |
| title_short | Proteomic Identification of Plasma Components in <i>Tachypleus tridentatus</i> and Their Effects on the Longitudinal Bone Growth Rate in Rats |
| title_sort | proteomic identification of plasma components in i tachypleus tridentatus i and their effects on the longitudinal bone growth rate in rats |
| topic | <i>Tachypleus tridentatus</i> longitudinal bone growth transcriptomics proteomics |
| url | https://www.mdpi.com/1660-3397/21/2/111 |
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