The association of serum clusterin levels and Clusterin rs11136000 polymorphisms with Alzheimer disease in a Turkish cohort
Objectives: Several large-scale genome association studies have shown that variants in the “Clusterin”' (CLU) gene are important risk factors for Alzheimer's disease (AD). It has also been shown that plasma CLU levels were elevated in patients with AD and associated with disease severity a...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Neurological Sciences and Neurophysiology |
| Subjects: | |
| Online Access: | http://www.nsnjournal.org/article.asp?issn=2636-865X;year=2020;volume=37;issue=3;spage=134;epage=140;aulast=Guven |
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| author | Gamze Guven Ebru Ozer Basar Bilgic Hasmet Hanagasi Hakan Gurvit Ebba Lohmann Nihan Erginel-Unaltuna |
| author_facet | Gamze Guven Ebru Ozer Basar Bilgic Hasmet Hanagasi Hakan Gurvit Ebba Lohmann Nihan Erginel-Unaltuna |
| author_sort | Gamze Guven |
| collection | DOAJ |
| description | Objectives: Several large-scale genome association studies have shown that variants in the “Clusterin”' (CLU) gene are important risk factors for Alzheimer's disease (AD). It has also been shown that plasma CLU levels were elevated in patients with AD and associated with disease severity and progression. In this study, we aimed to investigate whether the CLU rs11136000 polymorphism was associated with AD in our cohort of Turkish patients. We also evaluated the association of serum CLU levels and rs11136000 genotypes between patients and controls. Materials and Methods: Genotyping was performed in 327 patients who were diagnosed as having AD (mean age: 67.2 ± 10.8 years) and 344 controls (mean age: 57.7 ± 13.1 years). The rs11136000 genotypes were determined using quantitative real-time polymerase chain reaction with hydrolysis probes. Serum CLU levels were assessed in 25 patients with AD and 10 controls using enzyme-linked immunosorbent assay. Results: Our results showed no significant difference in genotype and allele frequencies of CLU rs11136000 polymorphisms between patients with AD and controls. Serum CLU levels in patients with AD did not differ from those of the controls. Furthermore, serum CLU levels showed no major difference between carriers of CC and TT + CT genotypes in the controls and patients with AD. Conclusion: Our results suggest that the CLU rs11136000 polymorphism is not associated with AD in our Turkish patients, and rs11136000 genotypes may not have an effect on serum CLU levels. |
| first_indexed | 2024-12-14T16:02:23Z |
| format | Article |
| id | doaj.art-c0e97c767c7b4f3bb106637dbfd735ab |
| institution | Directory Open Access Journal |
| issn | 2636-865X |
| language | English |
| last_indexed | 2024-12-14T16:02:23Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neurological Sciences and Neurophysiology |
| spelling | doaj.art-c0e97c767c7b4f3bb106637dbfd735ab2022-12-21T22:55:10ZengWolters Kluwer Medknow PublicationsNeurological Sciences and Neurophysiology2636-865X2020-01-0137313414010.4103/NSN.NSN_46_20The association of serum clusterin levels and Clusterin rs11136000 polymorphisms with Alzheimer disease in a Turkish cohortGamze GuvenEbru OzerBasar BilgicHasmet HanagasiHakan GurvitEbba LohmannNihan Erginel-UnaltunaObjectives: Several large-scale genome association studies have shown that variants in the “Clusterin”' (CLU) gene are important risk factors for Alzheimer's disease (AD). It has also been shown that plasma CLU levels were elevated in patients with AD and associated with disease severity and progression. In this study, we aimed to investigate whether the CLU rs11136000 polymorphism was associated with AD in our cohort of Turkish patients. We also evaluated the association of serum CLU levels and rs11136000 genotypes between patients and controls. Materials and Methods: Genotyping was performed in 327 patients who were diagnosed as having AD (mean age: 67.2 ± 10.8 years) and 344 controls (mean age: 57.7 ± 13.1 years). The rs11136000 genotypes were determined using quantitative real-time polymerase chain reaction with hydrolysis probes. Serum CLU levels were assessed in 25 patients with AD and 10 controls using enzyme-linked immunosorbent assay. Results: Our results showed no significant difference in genotype and allele frequencies of CLU rs11136000 polymorphisms between patients with AD and controls. Serum CLU levels in patients with AD did not differ from those of the controls. Furthermore, serum CLU levels showed no major difference between carriers of CC and TT + CT genotypes in the controls and patients with AD. Conclusion: Our results suggest that the CLU rs11136000 polymorphism is not associated with AD in our Turkish patients, and rs11136000 genotypes may not have an effect on serum CLU levels.http://www.nsnjournal.org/article.asp?issn=2636-865X;year=2020;volume=37;issue=3;spage=134;epage=140;aulast=Guvenalzheimer's diseaseclusterinpolymorphismserumturkey |
| spellingShingle | Gamze Guven Ebru Ozer Basar Bilgic Hasmet Hanagasi Hakan Gurvit Ebba Lohmann Nihan Erginel-Unaltuna The association of serum clusterin levels and Clusterin rs11136000 polymorphisms with Alzheimer disease in a Turkish cohort Neurological Sciences and Neurophysiology alzheimer's disease clusterin polymorphism serum turkey |
| title | The association of serum clusterin levels and Clusterin rs11136000 polymorphisms with Alzheimer disease in a Turkish cohort |
| title_full | The association of serum clusterin levels and Clusterin rs11136000 polymorphisms with Alzheimer disease in a Turkish cohort |
| title_fullStr | The association of serum clusterin levels and Clusterin rs11136000 polymorphisms with Alzheimer disease in a Turkish cohort |
| title_full_unstemmed | The association of serum clusterin levels and Clusterin rs11136000 polymorphisms with Alzheimer disease in a Turkish cohort |
| title_short | The association of serum clusterin levels and Clusterin rs11136000 polymorphisms with Alzheimer disease in a Turkish cohort |
| title_sort | association of serum clusterin levels and clusterin rs11136000 polymorphisms with alzheimer disease in a turkish cohort |
| topic | alzheimer's disease clusterin polymorphism serum turkey |
| url | http://www.nsnjournal.org/article.asp?issn=2636-865X;year=2020;volume=37;issue=3;spage=134;epage=140;aulast=Guven |
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