Mapping the functional anatomy and topography of the cardiac autonomic innervation for selective cardiac neuromodulation using MicroCT
Background: Vagus nerve stimulation (VNS) has gained great importance as a promising therapy for a myriad of diseases. Of particular interest is the therapy of cardiovascular diseases, such as heart failure or atrial fibrillation using selective cardiac VNS. However, there is still a lack of organ-s...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.968870/full |
| _version_ | 1797855024365174784 |
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| author | Bettina Kronsteiner Bettina Kronsteiner Lydia M. Zopf Patrick Heimel Patrick Heimel Patrick Heimel Gunpreet Oberoi Anne M. Kramer Paul Slezak Paul Slezak Wolfgang J. Weninger Wolfgang J. Weninger Bruno K. Podesser Attila Kiss Francesco Moscato Francesco Moscato Francesco Moscato |
| author_facet | Bettina Kronsteiner Bettina Kronsteiner Lydia M. Zopf Patrick Heimel Patrick Heimel Patrick Heimel Gunpreet Oberoi Anne M. Kramer Paul Slezak Paul Slezak Wolfgang J. Weninger Wolfgang J. Weninger Bruno K. Podesser Attila Kiss Francesco Moscato Francesco Moscato Francesco Moscato |
| author_sort | Bettina Kronsteiner |
| collection | DOAJ |
| description | Background: Vagus nerve stimulation (VNS) has gained great importance as a promising therapy for a myriad of diseases. Of particular interest is the therapy of cardiovascular diseases, such as heart failure or atrial fibrillation using selective cardiac VNS. However, there is still a lack of organ-specific anatomical knowledge about the fascicular anatomy and topography of the cardiac branch (CB), which diminishes the therapeutic possibilities for selective cardiac neuromodulation. Here, we established a topographical and anatomical map of the superior cardiac VN in two animal species to dissect cervical and cardiac VN morphology.Methods: Autonomic nerves including superior CBs were harvested from domestic pigs and New Zeeland rabbits followed by imaging with microcomputed tomography (µCT) and 3D rendering. The data were analyzed in terms of relevant topographical and anatomical parameters.Results: Our data showed that cardiac vagal fascicles remained separated from other VN fascicles up to 22.19 mm (IQR 14.02–41.30 mm) in pigs and 7.68 mm (IQR 4.06–12.77 mm) in rabbits from the CB point and then started merging with other fascicles. Exchanges of nerve fascicles between sympathetic trunk (ST) and VN were observed in 3 out of 11 nerves, which might cause additional unwanted effects in unselective VNS. Our 3D rendered digital model of the cardiac fascicles was generated showing that CB first remained on the medial side where it branched off the VN, as also shown in the µCT data of 11 pig nerves, and then migrated towards the ventromedial site the further it was traced cranially.Conclusion: Our data provided an anatomical map of the cardiac vagal branches including cervical VN and ST for future approaches of selective cardiac neurostimulation, indicating the best position of selective cardiac VNS just above the CB point. |
| first_indexed | 2024-04-09T20:15:44Z |
| format | Article |
| id | doaj.art-c0ed50a268c34cdb830c8d72cd8cab55 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-09T20:15:44Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-c0ed50a268c34cdb830c8d72cd8cab552023-03-31T08:49:30ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-09-011010.3389/fcell.2022.968870968870Mapping the functional anatomy and topography of the cardiac autonomic innervation for selective cardiac neuromodulation using MicroCTBettina Kronsteiner0Bettina Kronsteiner1Lydia M. Zopf2Patrick Heimel3Patrick Heimel4Patrick Heimel5Gunpreet Oberoi6Anne M. Kramer7Paul Slezak8Paul Slezak9Wolfgang J. Weninger10Wolfgang J. Weninger11Bruno K. Podesser12Attila Kiss13Francesco Moscato14Francesco Moscato15Francesco Moscato16Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, AustriaLudwig Boltzmann Institute for Cardiovascular Research, Vienna, AustriaAUVA Research Centre, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, AustriaAUVA Research Centre, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, AustriaAustrian Cluster for Tissue Regeneration, Vienna, AustriaKarl Donath Laboratory for Hard Tissue and Biomaterial Research, University Dental Clinic Vienna, Vienna, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, AustriaLudwig Boltzmann Institute for Cardiovascular Research, Vienna, AustriaAUVA Research Centre, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, AustriaAustrian Cluster for Tissue Regeneration, Vienna, AustriaDepartment of Anatomy, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, AustriaDivision of Anatomy, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, AustriaLudwig Boltzmann Institute for Cardiovascular Research, Vienna, AustriaLudwig Boltzmann Institute for Cardiovascular Research, Vienna, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, AustriaLudwig Boltzmann Institute for Cardiovascular Research, Vienna, AustriaAustrian Cluster for Tissue Regeneration, Vienna, AustriaBackground: Vagus nerve stimulation (VNS) has gained great importance as a promising therapy for a myriad of diseases. Of particular interest is the therapy of cardiovascular diseases, such as heart failure or atrial fibrillation using selective cardiac VNS. However, there is still a lack of organ-specific anatomical knowledge about the fascicular anatomy and topography of the cardiac branch (CB), which diminishes the therapeutic possibilities for selective cardiac neuromodulation. Here, we established a topographical and anatomical map of the superior cardiac VN in two animal species to dissect cervical and cardiac VN morphology.Methods: Autonomic nerves including superior CBs were harvested from domestic pigs and New Zeeland rabbits followed by imaging with microcomputed tomography (µCT) and 3D rendering. The data were analyzed in terms of relevant topographical and anatomical parameters.Results: Our data showed that cardiac vagal fascicles remained separated from other VN fascicles up to 22.19 mm (IQR 14.02–41.30 mm) in pigs and 7.68 mm (IQR 4.06–12.77 mm) in rabbits from the CB point and then started merging with other fascicles. Exchanges of nerve fascicles between sympathetic trunk (ST) and VN were observed in 3 out of 11 nerves, which might cause additional unwanted effects in unselective VNS. Our 3D rendered digital model of the cardiac fascicles was generated showing that CB first remained on the medial side where it branched off the VN, as also shown in the µCT data of 11 pig nerves, and then migrated towards the ventromedial site the further it was traced cranially.Conclusion: Our data provided an anatomical map of the cardiac vagal branches including cervical VN and ST for future approaches of selective cardiac neurostimulation, indicating the best position of selective cardiac VNS just above the CB point.https://www.frontiersin.org/articles/10.3389/fcell.2022.968870/fullvagus nerve stimulationcardiovascular diseasesfascicular anatomyselective cardiac neuromodulationmicrocomputed tomography3D rendering Kronsteiner et al. imaging of cardiac autonomic innervation |
| spellingShingle | Bettina Kronsteiner Bettina Kronsteiner Lydia M. Zopf Patrick Heimel Patrick Heimel Patrick Heimel Gunpreet Oberoi Anne M. Kramer Paul Slezak Paul Slezak Wolfgang J. Weninger Wolfgang J. Weninger Bruno K. Podesser Attila Kiss Francesco Moscato Francesco Moscato Francesco Moscato Mapping the functional anatomy and topography of the cardiac autonomic innervation for selective cardiac neuromodulation using MicroCT Frontiers in Cell and Developmental Biology vagus nerve stimulation cardiovascular diseases fascicular anatomy selective cardiac neuromodulation microcomputed tomography 3D rendering Kronsteiner et al. imaging of cardiac autonomic innervation |
| title | Mapping the functional anatomy and topography of the cardiac autonomic innervation for selective cardiac neuromodulation using MicroCT |
| title_full | Mapping the functional anatomy and topography of the cardiac autonomic innervation for selective cardiac neuromodulation using MicroCT |
| title_fullStr | Mapping the functional anatomy and topography of the cardiac autonomic innervation for selective cardiac neuromodulation using MicroCT |
| title_full_unstemmed | Mapping the functional anatomy and topography of the cardiac autonomic innervation for selective cardiac neuromodulation using MicroCT |
| title_short | Mapping the functional anatomy and topography of the cardiac autonomic innervation for selective cardiac neuromodulation using MicroCT |
| title_sort | mapping the functional anatomy and topography of the cardiac autonomic innervation for selective cardiac neuromodulation using microct |
| topic | vagus nerve stimulation cardiovascular diseases fascicular anatomy selective cardiac neuromodulation microcomputed tomography 3D rendering Kronsteiner et al. imaging of cardiac autonomic innervation |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.968870/full |
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