Physiological responses and transcriptomic analysis of StCPD gene overexpression in potato under salt stresses
IntroductionThe potato (Solanum tuberosum L.), one of the most vital food crops worldwide, is sensitive to salinity. Brassinosteroids (BRs) are crucial in tolerance to various abiotic stresses. The constitutive photomorphogenesis and dwarf (CPD) gene encodes C-3 oxidase, which is a rate-limiting enz...
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1297812/full |
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| author | Xiangyan Zhou Yanming Ma Rong Miao Caijuan Li Ziliang Liu Dan Zhang Sijin Chen Jiaqi Luo Wenhui Tang |
| author_facet | Xiangyan Zhou Yanming Ma Rong Miao Caijuan Li Ziliang Liu Dan Zhang Sijin Chen Jiaqi Luo Wenhui Tang |
| author_sort | Xiangyan Zhou |
| collection | DOAJ |
| description | IntroductionThe potato (Solanum tuberosum L.), one of the most vital food crops worldwide, is sensitive to salinity. Brassinosteroids (BRs) are crucial in tolerance to various abiotic stresses. The constitutive photomorphogenesis and dwarf (CPD) gene encodes C-3 oxidase, which is a rate-limiting enzyme that controls the synthesis of BRs.MethodsIn this study, we used StCPD gene overexpression (T) and un-transgenic (NT) plants obtained from our former research to illustrate adaptive resistance to salt stress at levels of phenotype; cell ultrastructure, physiology, and biochemistry; hormone; and transcription.ResultsResults showed the accumulation of 2,4-epibrassionolide (EBL) in T potatoes. We found that under high salt situations, the changed Na+/K+ transporter gene expression was linked with the prevalent ionic responses in T plants, which led to lower concentrations of K+ and higher concentrations of Na+ in leaves. Furthermore, RNA-sequencing (RNA-seq) data elucidated that gene expressions in NT and T plants were significantly changed with 200-mM NaCl treatment for 24 h and 48 h, compared with the 0-h treatment. Functional enrichment analysis suggested that most of the differentially expressed genes (DEGs) were related to the regulation of BR-related gene expression, pigment metabolism process, light and action, and plant hormone signal transduction.DiscussionThese findings suggested that StCPD gene overexpression can alleviate the damage caused by salt stress and enhance the salt resistance of potato plantlets. Our study provides an essential reference for further research on BR regulation of plant molecular mechanisms in potatoes with stress tolerance. |
| first_indexed | 2024-03-08T00:18:23Z |
| format | Article |
| id | doaj.art-c0edab601cc543fcb4e5f674890f416d |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-08T00:18:23Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c0edab601cc543fcb4e5f674890f416d2024-02-16T16:20:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-02-011510.3389/fpls.2024.12978121297812Physiological responses and transcriptomic analysis of StCPD gene overexpression in potato under salt stressesXiangyan Zhou0Yanming Ma1Rong Miao2Caijuan Li3Ziliang Liu4Dan Zhang5Sijin Chen6Jiaqi Luo7Wenhui Tang8State Key Laboratory of Aridland Crop Science/College of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science/College of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science/College of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science/College of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science/College of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science/College of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science/College of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaQinzhou District Agricultural Technology Comprehensive Service Center in Tianshui City, Tianshui, ChinaZhuanglang Agricultural Technology Extension Center in Pingliang City, Pingliang, ChinaIntroductionThe potato (Solanum tuberosum L.), one of the most vital food crops worldwide, is sensitive to salinity. Brassinosteroids (BRs) are crucial in tolerance to various abiotic stresses. The constitutive photomorphogenesis and dwarf (CPD) gene encodes C-3 oxidase, which is a rate-limiting enzyme that controls the synthesis of BRs.MethodsIn this study, we used StCPD gene overexpression (T) and un-transgenic (NT) plants obtained from our former research to illustrate adaptive resistance to salt stress at levels of phenotype; cell ultrastructure, physiology, and biochemistry; hormone; and transcription.ResultsResults showed the accumulation of 2,4-epibrassionolide (EBL) in T potatoes. We found that under high salt situations, the changed Na+/K+ transporter gene expression was linked with the prevalent ionic responses in T plants, which led to lower concentrations of K+ and higher concentrations of Na+ in leaves. Furthermore, RNA-sequencing (RNA-seq) data elucidated that gene expressions in NT and T plants were significantly changed with 200-mM NaCl treatment for 24 h and 48 h, compared with the 0-h treatment. Functional enrichment analysis suggested that most of the differentially expressed genes (DEGs) were related to the regulation of BR-related gene expression, pigment metabolism process, light and action, and plant hormone signal transduction.DiscussionThese findings suggested that StCPD gene overexpression can alleviate the damage caused by salt stress and enhance the salt resistance of potato plantlets. Our study provides an essential reference for further research on BR regulation of plant molecular mechanisms in potatoes with stress tolerance.https://www.frontiersin.org/articles/10.3389/fpls.2024.1297812/fullpotatoStCPD genesalt stresstranscriptomebrassinosteroids metabolism |
| spellingShingle | Xiangyan Zhou Yanming Ma Rong Miao Caijuan Li Ziliang Liu Dan Zhang Sijin Chen Jiaqi Luo Wenhui Tang Physiological responses and transcriptomic analysis of StCPD gene overexpression in potato under salt stresses Frontiers in Plant Science potato StCPD gene salt stress transcriptome brassinosteroids metabolism |
| title | Physiological responses and transcriptomic analysis of StCPD gene overexpression in potato under salt stresses |
| title_full | Physiological responses and transcriptomic analysis of StCPD gene overexpression in potato under salt stresses |
| title_fullStr | Physiological responses and transcriptomic analysis of StCPD gene overexpression in potato under salt stresses |
| title_full_unstemmed | Physiological responses and transcriptomic analysis of StCPD gene overexpression in potato under salt stresses |
| title_short | Physiological responses and transcriptomic analysis of StCPD gene overexpression in potato under salt stresses |
| title_sort | physiological responses and transcriptomic analysis of stcpd gene overexpression in potato under salt stresses |
| topic | potato StCPD gene salt stress transcriptome brassinosteroids metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1297812/full |
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