The effect of hypergravity on the shape of myoblasts
It is already known that micro gravity affects astronaut’s body. In contrary, hyper-gravity might affect myoblasts. In the present study, mouse muscle cells (C2C12) were cultured four five hours in hyper-gravitational environment (50 G, 100 G) using the centrifugal machine placed in the incubator. C...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Japanese |
| Published: |
The Japan Society of Mechanical Engineers
2018-01-01
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| Series: | Nihon Kikai Gakkai ronbunshu |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/transjsme/84/858/84_17-00368/_pdf/-char/en |
| _version_ | 1828126027412406272 |
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| author | Haruka HINO Takuya TAMURA Hiromi SUGIMOTO Yusuke TAKAHASHI Shigehiro HASHIMOTO |
| author_facet | Haruka HINO Takuya TAMURA Hiromi SUGIMOTO Yusuke TAKAHASHI Shigehiro HASHIMOTO |
| author_sort | Haruka HINO |
| collection | DOAJ |
| description | It is already known that micro gravity affects astronaut’s body. In contrary, hyper-gravity might affect myoblasts. In the present study, mouse muscle cells (C2C12) were cultured four five hours in hyper-gravitational environment (50 G, 100 G) using the centrifugal machine placed in the incubator. Cells were observed at the incubator microscope for 24 hours after stopping centrifugation. The contour of each cell at the time-lapse images was approximated to the ellipsoid and several parameters were calculated: the angle, the area, and the aspect ratio. Each cell tends to shrink from the extended shape under hyper-gravitational environment. The directions of the major axis at 50% of cells tilt to the parallel direction to the hypergravity. After 24 hours, 51% of cells tilt to the perpendicular direction of the hypergravity in the 50 G group. The result shows that the history of hyper-gravitational environment affects the shape of cells. |
| first_indexed | 2024-04-11T15:28:12Z |
| format | Article |
| id | doaj.art-c0f3a36fa7934e85ae6460102c4324f3 |
| institution | Directory Open Access Journal |
| issn | 2187-9761 |
| language | Japanese |
| last_indexed | 2024-04-11T15:28:12Z |
| publishDate | 2018-01-01 |
| publisher | The Japan Society of Mechanical Engineers |
| record_format | Article |
| series | Nihon Kikai Gakkai ronbunshu |
| spelling | doaj.art-c0f3a36fa7934e85ae6460102c4324f32022-12-22T04:16:11ZjpnThe Japan Society of Mechanical EngineersNihon Kikai Gakkai ronbunshu2187-97612018-01-018485817-0036817-0036810.1299/transjsme.17-00368transjsmeThe effect of hypergravity on the shape of myoblastsHaruka HINO0Takuya TAMURA1Hiromi SUGIMOTO2Yusuke TAKAHASHI3Shigehiro HASHIMOTO4Biomedical Engineering Laboratory Department of Mechanical Engineering, Kogakuin UniversityBiomedical Engineering Laboratory Department of Mechanical Engineering, Kogakuin UniversityBiomedical Engineering Laboratory Department of Mechanical Engineering, Kogakuin UniversityBiomedical Engineering Laboratory Department of Mechanical Engineering, Kogakuin UniversityBiomedical Engineering Laboratory Department of Mechanical Engineering, Kogakuin UniversityIt is already known that micro gravity affects astronaut’s body. In contrary, hyper-gravity might affect myoblasts. In the present study, mouse muscle cells (C2C12) were cultured four five hours in hyper-gravitational environment (50 G, 100 G) using the centrifugal machine placed in the incubator. Cells were observed at the incubator microscope for 24 hours after stopping centrifugation. The contour of each cell at the time-lapse images was approximated to the ellipsoid and several parameters were calculated: the angle, the area, and the aspect ratio. Each cell tends to shrink from the extended shape under hyper-gravitational environment. The directions of the major axis at 50% of cells tilt to the parallel direction to the hypergravity. After 24 hours, 51% of cells tilt to the perpendicular direction of the hypergravity in the 50 G group. The result shows that the history of hyper-gravitational environment affects the shape of cells.https://www.jstage.jst.go.jp/article/transjsme/84/858/84_17-00368/_pdf/-char/encentrifugal forcehypergravityc2c12orientation |
| spellingShingle | Haruka HINO Takuya TAMURA Hiromi SUGIMOTO Yusuke TAKAHASHI Shigehiro HASHIMOTO The effect of hypergravity on the shape of myoblasts Nihon Kikai Gakkai ronbunshu centrifugal force hypergravity c2c12 orientation |
| title | The effect of hypergravity on the shape of myoblasts |
| title_full | The effect of hypergravity on the shape of myoblasts |
| title_fullStr | The effect of hypergravity on the shape of myoblasts |
| title_full_unstemmed | The effect of hypergravity on the shape of myoblasts |
| title_short | The effect of hypergravity on the shape of myoblasts |
| title_sort | effect of hypergravity on the shape of myoblasts |
| topic | centrifugal force hypergravity c2c12 orientation |
| url | https://www.jstage.jst.go.jp/article/transjsme/84/858/84_17-00368/_pdf/-char/en |
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