Cultivation of <i>Chlorella sorokiniana</i> IPPAS C-1 in Flat-Panel Photobioreactors: From a Laboratory to a Pilot Scale
Flat-panel photobioreactors are effective systems for microalgae cultivation. This paper presents the growth characteristics of the microalgae <i>Chlorella sorokiniana</i> IPPAS C-1 as a result of three-stage scale-up cultivation in a specially designed cultivation system. First, <i&g...
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MDPI AG
2022-08-01
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| author | David A. Gabrielyan Maria A. Sinetova Boris V. Gabel Alexander K. Gabrielian Alexandra G. Markelova Margarita V. Rodionova Vladimir S. Bedbenov Natalia V. Shcherbakova Dmitry A. Los |
| author_facet | David A. Gabrielyan Maria A. Sinetova Boris V. Gabel Alexander K. Gabrielian Alexandra G. Markelova Margarita V. Rodionova Vladimir S. Bedbenov Natalia V. Shcherbakova Dmitry A. Los |
| author_sort | David A. Gabrielyan |
| collection | DOAJ |
| description | Flat-panel photobioreactors are effective systems for microalgae cultivation. This paper presents the growth characteristics of the microalgae <i>Chlorella sorokiniana</i> IPPAS C-1 as a result of three-stage scale-up cultivation in a specially designed cultivation system. First, <i>C. sorokiniana</i> was grown aseptically in 250 mL glass vessels; then, it was diluted and inoculated into a 5-liter flat-panel horizontal photobioreactor; and, at the last stage, the culture was diluted and inoculated into a 70-liter flat-panel vertical photobioreactor. In the presented cycle, the cultured biomass increased by 326 times in 13 days (from 0.6 to 195.6 g dw), with a final biomass concentration of 2.8 g dw L<sup>−1</sup>. The modes of semi-continuous cultivation were considered. The biomass harvest and dilution of the suspension were carried out either every day or every 3–4 days. For <i>C. sorokiniana</i> IPPAS C-1, a conversion coefficient of optical density values to dry biomass (g L<sup>−1</sup>) was refined through a factor of 0.33. The key parameters of the photobioreactors tested in this work are discussed. |
| first_indexed | 2024-03-09T23:23:43Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
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| last_indexed | 2024-03-09T23:23:43Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-c0f3bb2a16cb46bc8b2087b077f557502023-11-23T17:21:56ZengMDPI AGLife2075-17292022-08-01129130910.3390/life12091309Cultivation of <i>Chlorella sorokiniana</i> IPPAS C-1 in Flat-Panel Photobioreactors: From a Laboratory to a Pilot ScaleDavid A. Gabrielyan0Maria A. Sinetova1Boris V. Gabel2Alexander K. Gabrielian3Alexandra G. Markelova4Margarita V. Rodionova5Vladimir S. Bedbenov6Natalia V. Shcherbakova7Dmitry A. Los8K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, RussiaFlat-panel photobioreactors are effective systems for microalgae cultivation. This paper presents the growth characteristics of the microalgae <i>Chlorella sorokiniana</i> IPPAS C-1 as a result of three-stage scale-up cultivation in a specially designed cultivation system. First, <i>C. sorokiniana</i> was grown aseptically in 250 mL glass vessels; then, it was diluted and inoculated into a 5-liter flat-panel horizontal photobioreactor; and, at the last stage, the culture was diluted and inoculated into a 70-liter flat-panel vertical photobioreactor. In the presented cycle, the cultured biomass increased by 326 times in 13 days (from 0.6 to 195.6 g dw), with a final biomass concentration of 2.8 g dw L<sup>−1</sup>. The modes of semi-continuous cultivation were considered. The biomass harvest and dilution of the suspension were carried out either every day or every 3–4 days. For <i>C. sorokiniana</i> IPPAS C-1, a conversion coefficient of optical density values to dry biomass (g L<sup>−1</sup>) was refined through a factor of 0.33. The key parameters of the photobioreactors tested in this work are discussed.https://www.mdpi.com/2075-1729/12/9/1309<i>Chlorella</i>biomasscultivationflat-panel photobioreactormicroalgae |
| spellingShingle | David A. Gabrielyan Maria A. Sinetova Boris V. Gabel Alexander K. Gabrielian Alexandra G. Markelova Margarita V. Rodionova Vladimir S. Bedbenov Natalia V. Shcherbakova Dmitry A. Los Cultivation of <i>Chlorella sorokiniana</i> IPPAS C-1 in Flat-Panel Photobioreactors: From a Laboratory to a Pilot Scale Life <i>Chlorella</i> biomass cultivation flat-panel photobioreactor microalgae |
| title | Cultivation of <i>Chlorella sorokiniana</i> IPPAS C-1 in Flat-Panel Photobioreactors: From a Laboratory to a Pilot Scale |
| title_full | Cultivation of <i>Chlorella sorokiniana</i> IPPAS C-1 in Flat-Panel Photobioreactors: From a Laboratory to a Pilot Scale |
| title_fullStr | Cultivation of <i>Chlorella sorokiniana</i> IPPAS C-1 in Flat-Panel Photobioreactors: From a Laboratory to a Pilot Scale |
| title_full_unstemmed | Cultivation of <i>Chlorella sorokiniana</i> IPPAS C-1 in Flat-Panel Photobioreactors: From a Laboratory to a Pilot Scale |
| title_short | Cultivation of <i>Chlorella sorokiniana</i> IPPAS C-1 in Flat-Panel Photobioreactors: From a Laboratory to a Pilot Scale |
| title_sort | cultivation of i chlorella sorokiniana i ippas c 1 in flat panel photobioreactors from a laboratory to a pilot scale |
| topic | <i>Chlorella</i> biomass cultivation flat-panel photobioreactor microalgae |
| url | https://www.mdpi.com/2075-1729/12/9/1309 |
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