Molecular characterization and genetic diversity of Babesia bovis and Babesia bigemina of cattle in Thailand
Babesia bovis and B. bigemina are the most common tick-borne parasites that cause bovine babesiosis which effects livestock production, leading to economic losses in tropical and subtropical areas of the world. The aims of this study were to determine the molecular detection, genetic diversity and a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1065963/full |
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| author | Nitipon Srionrod Pornpiroon Nooroong Napassorn Poolsawat Sutthida Minsakorn Amaya Watthanadirek Witchuta Junsiri Siriphan Sangchuai Siriphan Sangchuai Runglawan Chawengkirttikul Panat Anuracpreeda |
| author_facet | Nitipon Srionrod Pornpiroon Nooroong Napassorn Poolsawat Sutthida Minsakorn Amaya Watthanadirek Witchuta Junsiri Siriphan Sangchuai Siriphan Sangchuai Runglawan Chawengkirttikul Panat Anuracpreeda |
| author_sort | Nitipon Srionrod |
| collection | DOAJ |
| description | Babesia bovis and B. bigemina are the most common tick-borne parasites that cause bovine babesiosis which effects livestock production, leading to economic losses in tropical and subtropical areas of the world. The aims of this study were to determine the molecular detection, genetic diversity and antigenicity prediction of B. bovis based on spherical body protein 2 (sbp-2) gene and B. bigemina based on rhoptry-associated protein 1a (rap-1a) gene in cattle in Thailand. By PCR assay, the molecular detection of B. bovis and B. bigemina infection revealed levels of 2.58% (4/155) and 5.80% (9/155), respectively. The phylograms showed that B. bovis sbp-2 and B. bigemina rap-1a sequences displayed 5 and 3 clades with similarity ranging between 85.53 to 100% and 98.28 to 100%, respectively, when compared within Thailand strain. Diversity analysis of sbp-2 and rap-1a sequences showed 18 and 4 haplotypes, respectively. The entropy analysis illustrated 104 and 7 polymorphic sites of sbp-2 and rap-1a nucleic acid sequences, respectively, while those of sbp-2 and rap-1a amino acid sequences showed 46 and 4 high entropy peaks, respectively. Motifs analysis exhibited the distribution and conservation among sbp-2 and rap-1a sequences. The continuous and discontinuous B-cell epitopes have also been evaluated in this work. Therefore, our findings may be used to ameliorate the understanding inputs of molecular phylogeny, genetic diversity and antigenicity of B. bovis and B. bigemina Thailand stains. |
| first_indexed | 2024-04-11T15:06:28Z |
| format | Article |
| id | doaj.art-c0f533699ac641d29fda14f637a07b28 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-11T15:06:28Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-c0f533699ac641d29fda14f637a07b282022-12-22T04:16:47ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-11-011210.3389/fcimb.2022.10659631065963Molecular characterization and genetic diversity of Babesia bovis and Babesia bigemina of cattle in ThailandNitipon Srionrod0Pornpiroon Nooroong1Napassorn Poolsawat2Sutthida Minsakorn3Amaya Watthanadirek4Witchuta Junsiri5Siriphan Sangchuai6Siriphan Sangchuai7Runglawan Chawengkirttikul8Panat Anuracpreeda9Parasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, ThailandParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, ThailandParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, ThailandParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, ThailandParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, ThailandParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, ThailandParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, ThailandDepartment of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok, ThailandParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, ThailandBabesia bovis and B. bigemina are the most common tick-borne parasites that cause bovine babesiosis which effects livestock production, leading to economic losses in tropical and subtropical areas of the world. The aims of this study were to determine the molecular detection, genetic diversity and antigenicity prediction of B. bovis based on spherical body protein 2 (sbp-2) gene and B. bigemina based on rhoptry-associated protein 1a (rap-1a) gene in cattle in Thailand. By PCR assay, the molecular detection of B. bovis and B. bigemina infection revealed levels of 2.58% (4/155) and 5.80% (9/155), respectively. The phylograms showed that B. bovis sbp-2 and B. bigemina rap-1a sequences displayed 5 and 3 clades with similarity ranging between 85.53 to 100% and 98.28 to 100%, respectively, when compared within Thailand strain. Diversity analysis of sbp-2 and rap-1a sequences showed 18 and 4 haplotypes, respectively. The entropy analysis illustrated 104 and 7 polymorphic sites of sbp-2 and rap-1a nucleic acid sequences, respectively, while those of sbp-2 and rap-1a amino acid sequences showed 46 and 4 high entropy peaks, respectively. Motifs analysis exhibited the distribution and conservation among sbp-2 and rap-1a sequences. The continuous and discontinuous B-cell epitopes have also been evaluated in this work. Therefore, our findings may be used to ameliorate the understanding inputs of molecular phylogeny, genetic diversity and antigenicity of B. bovis and B. bigemina Thailand stains.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1065963/fullBabesia bovisBabesia bigeminaBabesiosisnested PCRgenetic diversitycattle |
| spellingShingle | Nitipon Srionrod Pornpiroon Nooroong Napassorn Poolsawat Sutthida Minsakorn Amaya Watthanadirek Witchuta Junsiri Siriphan Sangchuai Siriphan Sangchuai Runglawan Chawengkirttikul Panat Anuracpreeda Molecular characterization and genetic diversity of Babesia bovis and Babesia bigemina of cattle in Thailand Frontiers in Cellular and Infection Microbiology Babesia bovis Babesia bigemina Babesiosis nested PCR genetic diversity cattle |
| title | Molecular characterization and genetic diversity of Babesia bovis and Babesia bigemina of cattle in Thailand |
| title_full | Molecular characterization and genetic diversity of Babesia bovis and Babesia bigemina of cattle in Thailand |
| title_fullStr | Molecular characterization and genetic diversity of Babesia bovis and Babesia bigemina of cattle in Thailand |
| title_full_unstemmed | Molecular characterization and genetic diversity of Babesia bovis and Babesia bigemina of cattle in Thailand |
| title_short | Molecular characterization and genetic diversity of Babesia bovis and Babesia bigemina of cattle in Thailand |
| title_sort | molecular characterization and genetic diversity of babesia bovis and babesia bigemina of cattle in thailand |
| topic | Babesia bovis Babesia bigemina Babesiosis nested PCR genetic diversity cattle |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1065963/full |
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